N boc ethylenediamine

PLGA (or poly (lactic-co-glycolic) acid at 40–75 g/mol, used 50:50), chitosan (Chi) (100–300 g/mol), polyvinyl alcohol (PVA) (31–50 g/mol) 87–89% hydrolyzed, fluorescein isothiocyanate (FITC), N-Boc-ethylenediamine, N,N-diisopropylethylamine (DIPEA),trifluoroacetic acid (TFA), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo [4,5-b]pyridinium 3-oxid hexafluorophosphate) (HATU), 4′,6′-diamidino-2-phenylindole (DAPI), boric acid, and potassium iodine were acquired from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade ethyl acetate, acetone, DCM, ethanol, and acetonitrile were purchased from Mallinckrodt Baker (Pittsburgh, NJ, USA).

Ligand 1 (LacNAc; Galβ4GlcNAc) was obtained from Carbosynth (Compton, UK). The preparation of β-galactosidase from Bacillus circulans Biolacta^® was from Daiwa Kasei (Aichi, Japan). β-Alanine, 2,2′-azobisisobutyronitrile (AIBN), 2-cyanopropan-2-yl dithiobenzoate, 2-thiazoline-2-thiol (CTA), CuBr, methyl 3,5-dihydroxybenzoate, methyl-3,4,5-trihydroxybenzoate, N-Boc-ethylenediamine, and N,N-diisopropylethylamine (DIPEA) were obtained from Sigma-Aldrich (Prague, Czech Republic). Propargyl bromide was purchased from Acros Organics (Pardubice, Czech Republic), and trifluoroacetic acid (TFA) was acquired from Iris Biotech (Marktredwitz, Germany).

Sodium hyaluronate (100–150 kDa) was purchased from Lifecore Biomedical. Dialysis membranes Spectra/Por (MW cut off 3.5 kDa) were purchased from SpectrumLabs. Tetrahydrofuran dry, hydrochloric acid (HCl) 37%, Dulbelco’s phosphate buffer saline (DPBS) and sodium chloride were purchased from Fischer Scientific. 3-(Trimethoxysilyl)propyl methacrylate, N-Boc-ethylenediamine, triethylamine anhydrous, acryloyl chloride, dioxane, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, 1-hydroxybenzotriazole hydrate, acetonitrile anhydrous, sodium hydrogen carbonate and photo-initiator Irgacure 2959 were purchased from Sigma Aldrich. Calcein AM, propidium iodide, minimum essential medium (MEM) without phenol red, Dulbecco’s modified Eagle medium (DMEM), high glucose Glutamax and fetal bovine serum (FBS) were purchased from Fisher Scientific. RGDSC, 5FAM-RGDSC, GCGYRGDSPG and 5FAM-GCGYRGDSPG peptides were purchased from Innovagen AB. Glass coverslips and absolute ethanol were purchased from VWR. Plus One Repel-Silane ES was purchased from GE Healthcare Life Sciences.