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Alexafluor 488 goatanti rabbit or goat anti mouse antibody

Manufactured by Thermo Fisher Scientific
Sourced in United States

AlexaFluor 488 goat anti-rabbit or goat anti-mouse antibody is a fluorescently labeled secondary antibody used for detection and visualization in various immunoassays and imaging applications.

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2 protocols using alexafluor 488 goatanti rabbit or goat anti mouse antibody

1

Immunofluorescence Imaging of Mitochondrial Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Coverslips were fixed in 4% paraformaldehyde overnight at 4°C and
blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody
(1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500
dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat
anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular
Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus
Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60×
UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room
temperature.
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2

Mitochondrial Dynamics Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Coverslips were fixed in 4% paraformaldehyde overnight at 4° C and blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody (1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500 dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60× UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room temperature.
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