blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody
(1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500
dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat
anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular
Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus
Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60×
UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room
temperature.