Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM-F12), penicillin/streptomycin and fetal bovine serum (FBS) were obtained from Gibco. Collagenase P were obtained from Roche. Anti-human CD34-PE, CD45-FITC, HLA-DR-FITC, CD44-FITC, CD73-PE, CD90-FITC, and were purchased from BD Biosciences. Adipogenic and osteogenic inducing medium from Cyagen. Matrigel were obtained from Corning. Trizol Reagent were purchased by Invitrogen. All-In-One RT MasterMix Kit, EvaGreen qPCR MasterMix Kit were obtained from abmGood. Cell-tracker dye CM-DiI were purchased from Invitrogen.
Cell tracker dye cm dii
Manufactured by Thermo Fisher Scientific
The Cell-tracker dye CM-DiI is a fluorescent dye used for labeling and tracking cells. It can be used to stain cell membranes and is suitable for long-term cell lineage tracing and in vivo cell tracking applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cd45 fitc, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
Hla dr fitc, by BD (1 mentions)
Cd44 fitc, by BD (1 mentions)
Cd90 fitc, by BD (1 mentions)
Anti human cd34 pe, by BD (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Matrigel, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Collagenase p, by Roche (1 mentions)
All in one rt mastermix kit, by Abmgood (1 mentions)
Evagreen qpcr mastermix kit, by Abmgood (1 mentions)
2 protocols using cell tracker dye cm dii
1
Isolation and Characterization of Mesenchymal Stem Cells
2
EV Uptake Measurement in Cells
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EV suspensions were incubated with 2 μM CellTracker dye CM-DiI (Invitrogen) in Hank’s balanced salt solution for 5 min, at 37°C, and subsequently placed on ice for an additional 15 min. Excess dye was removed using an exosome spin column (MW 100,000), as per the manufacturer’s instructions. For uptake analysis, stained EVs from 0.5 ml of medium (approximately 0.5 × 109 EVs) were cultured with HUVECs and PBMCs for 2 hours. Cells were then rinsed twice with PBS, and then fixed with 4% paraformaldehyde. Immunofluorescence images were captured with a confocal fluorescence microscope (Nikon A1R).
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