The synthesized ZnO NPs were characterized using various spectroscopic and microscopic techniques. UV–visible spectrum was evaluated using UV–Visible spectrophotometer (Shimadzu UV-2450) and the spectrum was recorded between 300 and 800 nm. Hydrodynamic (Z-average) size and polydispersity index (PDI) of the synthesized ZnO NPs were evaluated by Zeta sizer instrument (Malvern Zetasizer Nano ZS90), and the results were acquired by the Malvern ZS nano software. Fourier transform infrared (FTIR) analysis of the NPs was carried out with Fourier transform spectrometer (Shimadzu FT-IR Prestige-21 Model) at a frequency range of 4,000–500 cm−1. Crystalline structure was analyzed using X-ray diffractometer (Rigaku ZSX Primus II). Morphological analysis of the synthesized ZnO NPs coated with platinum was carried out using scanning electron microscope (SEM) (JOEL JSM 6335-F) equipped with 150 kV acceleration voltage, and energy-dispersive X-ray spectroscopy (EDS) (Oxford Instruments AZTEC EDS) attached to the same instrument was used to ascertain the elemental composition and purity of the synthesized ZnO NPs.
Aztec eds
Manufactured by Oxford Instruments
Sourced in United Kingdom, Japan
AZTEC EDS is an energy-dispersive X-ray spectroscopy (EDS) system developed by Oxford Instruments. It is designed to provide elemental analysis and chemical composition information for specimens in a scanning electron microscope (SEM) or other similar analytical instruments.
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3 protocols using aztec eds
1
Characterization of Synthesized ZnO Nanoparticles
2
Characterization of SD-MBG and BMP-2 Particles
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The morphology of SD-MBG and BMP-2 loaded SD-MBG (SD-MBG + BMP-2, 20 µg BMP-2 per mg SD-MBGs) particles was analyzed by field-emission scanning electron microscopy (FE-SEM) using a ZEISS MERLIN (Carl Zeiss, Oberkochen, Germany) instrument. For FE-SEM observations, 5 mg of both SD-MBG and SD-MBG + BMP-2 powders were dispersed on a conductive carbon tape and coated with a chromium layer. Compositional analysis of the powders was performed by energy dispersive spectroscopy (EDS) using an AZtec EDS (Oxford instruments, Abingdon, UK). The spectra were collected on powders dispersed on carbon tape by analyzing an area of 75 × 50 µm.
3
Biosynthesized ZnO Nanoparticle Characterization
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The biosynthesized ZnO NPsAL ZnO NPs were characterized using various spectroscopic and microscopic techniques. A UV-visible spectrophotometer (Shimadzu UV-2450, Duisberg, Germany) was used to evaluate the UV-visible spectrum and recorded between 300 and 800nm (Figure 6 ). The crystalline structure was analyzed using an X-ray diffractometer (Rigaku ZSX Primus II, Wilmington, MA, USA) (Figure 7 ). Morphological analysis of the synthesized ZnO NPs coated with platinum was analyzed using a scanning electron microscope (SEM) (JOEL JSM 6335-F, Tokyo, Japan), X-ray spectroscopy (EDS) (Oxford Instruments AZTEC EDS, Osaka, Japan) affixed to the same instrument was used to ascertain the elemental composition (Figure 8 ). Size of the synthesized nanoparticles was evaluated using the Malvern Zetasizer Nano ZS90 instrument. The average size of the nanoparticles was found to be 66.25 nm with a 0.262 polydispersity index (Figure 9 ). Uv-Vis, XRD, and SEM-EDS, and Zeta sizer results of biosynthesized ZnO NPsAL were previously reported in our recent studies [10 (link)].
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