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Pe cy 7 cd4

Manufactured by Thermo Fisher Scientific
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PE-Cy™7-CD4 is a fluorochrome-conjugated antibody for use in flow cytometry applications. It is designed to specifically bind to the CD4 cell surface receptor, which is expressed on a subset of T lymphocytes. The PE-Cy™7 fluorescent label allows for the detection and enumeration of CD4-positive cells.

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Lab products found in correlation

Apc cy 7 cd8 557834, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Facsdiva, by BD (2 mentions) Anti ly6g apc, by BioLegend (2 mentions) Ly6c fitc, by BioLegend (2 mentions) Apcef780 cd8, by Thermo Fisher Scientific (2 mentions) Bv421 cxcr4, by BioLegend (2 mentions) Cd11b ef450, by Thermo Fisher Scientific (2 mentions) Pe cxcr2, by BioLegend (2 mentions) Cd19 pecy5, by Thermo Fisher Scientific (2 mentions) Peef610 cd11c, by Thermo Fisher Scientific (2 mentions) Pevio770 cd64, by Miltenyi Biotec (2 mentions) Percpef710 mhcii, by Thermo Fisher Scientific (2 mentions) Siglec f, by BD (2 mentions)

4 protocols using pe cy 7 cd4

1

Lymphocyte Population Analysis by FACS

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The proportions of different lymphocyte populations were determined through FACS analysis (BD FACSCanto II), and the results were analyzed with BD FACS Diva, version 6.1.3 (BD Biosciences, Franklin Lakes, NJ, USA). The antibodies employed in this analysis were FITC-CD14 (cat. no. 11-0149), PerCP-Cy5.5-CD3 (45-0037), APC-CD28 (17-0289) (eBioscience, San Diego, CA, USA), PE-Cy™7-CD4 (cat. no. 557852) and APC-Cy™7-CD8 (557834) (BD Biosciences).
Marttila S., Kananen L., Jylhävä J., Nevalainen T., Hervonen A., Jylhä M, & Hurme M. (2015). Length of paternal lifespan is manifested in the DNA methylome of their nonagenarian progeny. Oncotarget, 6(31), 30557-30567.
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2

Multiparameter Flow Cytometry Panel

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APC: anti-Ly6G (clone: 1A8, Biolegend); APCeF780: CD8 (clone: 53–6.7, eBioscience), CD45 (clone: 30-F11, eBioscience), CD24 (clone: M1/69, eBioscience); BV421: CXCR4 (clone: L276F12, Biolegend); eF450: CD11b (clone: M1/70, Invitrogen); FITC: Ly6C (clone: HK1.4, Biolegend); PE: CXCR2 (clone: SA044G4, Biolegend), SiglecF (clone: E50–2440, BD bioscience); PECy5: CD19 (clone: 1D3, eBioscience); PECy7: CD4 (clone: GK1.5, eBioscience); PEeF610: CD11c (clone N418, eBioscience); PEVio770: CD64 (clone: REA286, Miltenyi Biotec); PerCPeF710: MHCII (clone: M5/114.15.2, eBioscience).
Kulkarni U., Zemans R.L., Smith C.A., Wood S., Deng J.C, & Goldstein D.R. (2019). Excessive neutrophil levels in the lung underlie the age-associated increase in influenza mortality. Mucosal immunology, 12(2), 545-554.
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3

Lymphocyte Profiling by FACS Analysis

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The proportions of different lymphocyte populations were determined through FACS analysis (BD FACSCanto II), and the results were analysed with BD FACS Diva, version 6.1.3 (BD Biosciences, Franklin Lakes, NJ, USA). The antibodies employed in this analysis were FITC-CD14 (cat. no. 11-0149), PerCP-Cy5.5-CD3 (45-0037), APC-CD28 (17-0289) (eBioscience, San Diego, CA, USA), PE-Cy™7-CD4 (cat. no. 557852) and APC-Cy™7-CD8 (557834) (BD Biosciences).
Marttila S., Kananen L., Häyrynen S., Jylhävä J., Nevalainen T., Hervonen A., Jylhä M., Nykter M, & Hurme M. (2015). Ageing-associated changes in the human DNA methylome: genomic locations and effects on gene expression. BMC Genomics, 16(1), 179.
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4

Multiparameter Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
APC: anti-Ly6G (clone: 1A8, Biolegend); APCeF780: CD8 (clone: 53–6.7, eBioscience), CD45 (clone: 30-F11, eBioscience), CD24 (clone: M1/69, eBioscience); BV421: CXCR4 (clone: L276F12, Biolegend); eF450: CD11b (clone: M1/70, Invitrogen); FITC: Ly6C (clone: HK1.4, Biolegend); PE: CXCR2 (clone: SA044G4, Biolegend), SiglecF (clone: E50–2440, BD bioscience); PECy5: CD19 (clone: 1D3, eBioscience); PECy7: CD4 (clone: GK1.5, eBioscience); PEeF610: CD11c (clone N418, eBioscience); PEVio770: CD64 (clone: REA286, Miltenyi Biotec); PerCPeF710: MHCII (clone: M5/114.15.2, eBioscience).
Kulkarni U., Zemans R.L., Smith C.A., Wood S., Deng J.C, & Goldstein D.R. (2019). Excessive neutrophil levels in the lung underlie the age-associated increase in influenza mortality. Mucosal immunology, 12(2), 545-554.
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