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Bin1 99d

Manufactured by Merck Group
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Sourced in United States

BIN1 (99D) is a laboratory equipment product designed for use in scientific research and analysis. It serves as a storage and organization device for various samples or materials. The core function of BIN1 (99D) is to provide a secure and controlled environment for the storage and handling of such items.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Enhanced chemiluminesence solutions, by Thermo Fisher Scientific (2 mentions) Psd 95, by Merck Group (2 mentions) 0.45 μm nitrocellulose membrane, by Merck Group (2 mentions) Chemidoc imager, by Bio-Rad (2 mentions) Tau 1, by Merck Group (2 mentions) Nupage 4 12, by Thermo Fisher Scientific (2 mentions) Bis tris gel, by Thermo Fisher Scientific (2 mentions) Synaptophysin, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions) β actin, by Abcam (1 mentions) β actin ac 15, by Abcam (1 mentions)

2 protocols using bin1 99d

1

Western Blot Analysis of Synaptic Proteins

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Protein concentrations of samples were determined using a BCA protein assay kit (Thermo Fisher Scientific) and Ponceau Red staining of membranes. Equal protein amounts were electophoresed on 10% tris-glycine SDS-polyacrylamide gels, Nu-Page 4–12% or 10% bis–tris gels (Invitrogen), transferred to 0.45-μm nitrocellulose membrane (Millipore, MA, USA) and immunoblotted using standard methods. Primary antibodies were BIN1 (99D; Millipore), GST (GE Healthcare, IL, USA), total tau (total human tau; Agilent), Tau-1 (Millipore), PHF1 (Peter Davies, Donald and Barbara Zucker School of Medicine at Hofstra, Northwell), N-methyl-d-aspartate subunit 2B (06-600; Millipore), β-actin (ac15; Abcam), synaptophysin (sc17750; Santa Cruz), PSD95 (MAB 1596; Millipore) and Fyn (HPA023887; Sigma). The bound horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) were detected using enhanced chemiluminesence solutions (Thermo Fisher Scientific) and visualized using a ChemiDoc imager (Bio-Rad, CA, USA). Densitometric analysis was performed using FIJI.
Glennon E.B., Lau D.H., Gabriele R.M., Taylor M.F., Troakes C., Opie-Martin S., Elliott C., Killick R., Hanger D.P., Perez-Nievas B.G, & Noble W. (2020). Bridging integrator 1 protein loss in Alzheimer’s disease promotes synaptic tau accumulation and disrupts tau release. Brain Communications, 2(1), fcaa011.
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2

Quantitative Protein Analysis via Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentrations of samples were determined using a BCA protein
assay kit (Thermo Fisher Scientific) and Ponceau Red staining of membranes.
Equal protein amounts were electophoresed on 10 %
tris-glycine-SDS-polyacrylamide gels, Nu-Page 4-12 % or 10 % bistris gels
(Invitrogen), transferred to 0.45 μm nitrocellulose membrane (Millipore,
MA, USA), and immunoblotted using standard methods. Primary antibodies were BIN1
(99D, Millipore), GST (GE Healthcare, IL, USA), total tau (total human tau,
Agilent), Tau-1 (Millipore), PHF1 (Peter Davies, Donald and Barbara Zucker
School of Medicine at Hofstra, Northwell), N-methyl-D-aspartate subunit 2B
(NR2B) (06-600, Millipore), β-actin (ac15, Abcam), synaptophysin
(sc17750, Santa Cruz), PSD95 (MAB 1596, Millipore), fyn (HPA023887, Sigma).
Bound horseradish peroxidise (HRP)-conjugated secondary antibodies (GE
Healthcare) were detected using enhanced chemiluminesence solutions (Thermo
Fisher Scientific) and visualized using a chemi-doc imager (Bio-rad, CA, USA).
Densitometric analysis was performed using FIJI.
Glennon E.B., Lau D.H., Gabriele R.M., Taylor M.F., Troakes C., Opie-Martin S., Elliott C., Killick R., Hanger D.P., Perez-Nievas B.G, & Noble W. (2020). Bridging integrator 1 protein loss in Alzheimer’s disease promotes synaptic tau accumulation and disrupts tau release. Brain Communications, 2(1), fcaa011.
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