Cobas 6000

Manufactured by Roche

Sourced in Switzerland, Germany, United States, Japan, Denmark, Italy, France, United Kingdom, Belgium, Norway, China, Israel, Poland, Netherlands, Sweden

The Cobas 6000 is an automated clinical chemistry and immunoassay analyzer system designed for high-volume laboratory testing. It combines the features of two separate instruments, the Cobas c 501 module for clinical chemistry and the Cobas e 601 module for immunoassays, into a single integrated platform. The Cobas 6000 system is capable of performing a wide range of diagnostic tests, including biochemical, immunological, and specialty assays.

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Lab products found in correlation

Modular p, by Roche (23 mentions) Cobas 8000, by Roche (21 mentions) Cobas e411, by Roche (10 mentions) Cobas integra 400 plus, by Roche (9 mentions) Au5800, by Beckman Coulter (9 mentions) Dxh 800, by Beckman Coulter (6 mentions) Cobas c 501, by Roche/Hitachi (6 mentions) E601 module, by Roche (5 mentions) Advia centaur, by Siemens (5 mentions) Cardiophase hscrp, by Siemens (5 mentions) Beckman synchron lx20, by Roche (4 mentions) Modular p chemistry analyzer, by Roche (4 mentions) Cobas e601 module, by Roche (4 mentions) Sedivue dx, by IDEXX (4 mentions) Advia 120, by Siemens (4 mentions) Cobas integra 700 800, by Roche (4 mentions) Cell dyn 3700, by Abbott (4 mentions) Bn 2 system, by Siemens (4 mentions) Xn 9000, by Sysmex (4 mentions) Xt 2000i, by Sysmex (4 mentions)

Close spelling variants of this product

Cobas 6000 analyzer (196 citations) Cobas 6000/c501 (75 citations) Cobas® 6000 analyzer series (33 citations) Cobas 6000 system (30 citations) COBAS 6000 autoanalyzer (12 citations) Cobas 6000/8000 (9 citations) Cobas 6000 c501 module (6 citations) Cobas Integra 6000 (3 citations) Cobas 6000 and 8000 (3 citations) Cobas 6000/e601 analyzer (3 citations)

699 protocols using cobas 6000

Biomarker Panel for Metabolic Health

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Blood (15 mL) and urine were obtained. A total of 5 ml of whole blood were split into two purple top tubes, one frozen for future studies, and the other refrigerated and sent on the same day for HbA1c measurement. The remainder of the blood (10 ml) was centrifuged and the serum was split into several 1 mL Eppendorf tubes. One tube was refrigerated and sent on the same day for glucose measurement. The remaining tubes were kept frozen at − 80 °C for future assays. A fingerstick glucose was obtained at the same time as the laboratory tests.
Laboratory tests were subsequently done using the following methodology: HbA1c by HPLC (Bio-Rad); FPG by Enzymatic method (Cobas 6000, Roche); Serum and Urine Creatinine by the Jaffe rate method (Cobas 6000, Roche); Urine microalbumin by Immunoturbidimetry (Cobas 6000, Roche); TSH, 25(OH) and vitamin D by electrochemiluminescence Immunoassay (ECLIA) (Cobas e 411, Roche), and insulin by immunoassay (Cobas e 411, Roche). Levels of triglycerides, HDL-C, and total cholesterol were measured using enzymatic colorimetric method (Cobas 6000, Roche), and of LDL-C using Coupled Classic precipitation (Cobas 6000, Roche). CRP was measured by Immunoturbidimetric assay (Cobas 6000, Roche).

Nasrallah M.P., Elbejjani M., Nasreddine L., Chami H., Ismaeel H., Fleifel M., Al Zahraa Chokor F, & Tamim H. (2022). Incidence of diabetes and its predictors in the Greater Beirut Area: a five-year longitudinal study. Diabetology & Metabolic Syndrome, 14, 67.

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Comprehensive Metabolic Profiling Protocol

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Glucose was measured spectrophotometrically, using an enzymatic method with hexokinase (Cobas 6000, Roche, Mannheim, Germany). Total calcium was measured spectrophotometrically, using an NM-BAPTA method
(5-nitro-5'-methyl-(1,2-bis(o-aminophenoxy) ethan-N,N,N',N'-tetraacetic acid) (Cobas 6000, Roche, Mannheim, Germany).
Thyreotropin (TSH), free thyroxine fraction (fT4), free triiodothyronine (fT3), C peptide (Cp), insulin, parathyroid hormone (PTH) and vitamin D (25(OH)D total -25OHD 2 +25OHD 3 ) were measured by immunoanalytic ECLIA methods (Cobas 6000, Roche, Mannheim, Germany).
C reactive protein (CRP) was measured spectrophotometrically, using an immunoturbidimetric assay (Cobas 6000, Roche, Mannheim, Germany). Sex hormone binding globuline (SHBG) was measured by immunoradiometric assay (SHBG IRMA KIT) (Beckman Coulter Inc., Chaska, Minnesota, USA). Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) were measured by radioimunoassay (RIA DHEA) (Beckman Coulter, Prague, Czech Republic).
Thyroid auto-antibodies (T-Ab) -antibodies to thyroid peroxidase (anti TPO) and antibodies to thyreoglobulin (anti Tg) were measured using an ELISA method (Aesku.Diagnostics, Wendelsheim, Germany) on an Immunomat BASE (Serion Immunologics, Germany).

Vondra K., Bílek R., Matucha P., Salátová M., Vosátková M., Stárka L, & Hampl R. (2017). Vitamin D supplementation changed relationships, not levels of metabolic-hormonal parameters in autoimmune thyroiditis. Physiological research, 66(Suppl 3).

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Fasting Biomarker Measurement Protocol

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Blood samples were collected after an overnight fast and processed for further biochemical analysis. Plasma glucose was measured by enzymatic hexokinase method (Cobas 6000, Roche Diagnostics, Germany) and glycated haemoglobin (HbA1c) by turbidimetric inhibition immunoassay (Cobas 6000, Roche Diagnostics, Germany) this being a National Glycohaemoglobin Standardisation Programme (NGSP) certified method. Creatinine levels were measured using the standardized creatinine assay (Cobas 6000, Roche Diagnostics, Germany). Total cholesterol (TC), high density lipoprotein cholesterol (HDL-c), triglycerides (TG) and γ-glutamyltransferase (GGT) were estimated by enzymatic colorimetric methods (Cobas 6000, Roche Diagnostics). Low density lipoprotein cholesterol (LDL-c) was calculated using Friedewald’s formula [40 (link)]. Insulin was determined by a microparticle enzyme immunoassay (Axsym, Abbot). C-reactive protein (CRP) was measured by a high-sensitivity CRP assay, based on the highly sensitive Near Infrared Particle Immunoassay rate methodology (Immage® Immunochemistry System; Beckman Coulter), with a lower limit of detection of 0.2 mg/L.

Vergotine Z., Yako Y.Y., Kengne A.P., Erasmus R.T, & Matsha T.E. (2014). Proliferator-activated receptor gamma Pro12Ala interacts with the insulin receptor substrate 1 Gly972Arg and increase the risk of insulin resistance and diabetes in the mixed ancestry population from South Africa. BMC Genetics, 15, 10.

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Estimating Glomerular Filtration Rate and Urinary Albumin Excretion

Cited 1 time

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Glomerular filtration rate (GFR) was estimated with the CKD-EPI equation based on serum creatinine. Serum creatinine was measured with a Jaffé method traceable to the isotope-dilution mass spectrometry (due to a change of supplier, 2 instruments were used in the study, the Beckman Synchron LX20 and the Roche Cobas 6000).
We assessed urinary albumin excretion (UAE) in two 24-h urine samples, as previously described [13 (link), 15 (link)]. Briefly, urinary albumin concentration was measured with a standard immunoturbidimetric assay by an automatic analyzer (due to a change of supplier, by the Beckman Synchron LX20 and the Roche Cobas 6000) and multiplied by collection volume to obtain 24-h UAE. A urinary albumin concentration below the detection limit of the assay was set at 1.5 mg/L (2 mg/L for the Beckman Synchron LX20 and 3 mg/L for the Roche Cobas 6000) before multiplying by collection volume. Only urine collections with a collection time between 20 and 28 h were considered valid. If needed, UAE was extrapolated to 24-h excretion. For this study, UAE was preferably based on the average of two 24-h urine collections.

van Dinther M., Schram M.T., Jansen J.F., Backes W.H., Houben A.J., Berendschot T.T., Schalkwijk C.G., Stehouwer C.D., van Oostenbrugge R.J, & Staals J. (2021). Extracerebral microvascular dysfunction is related to brain MRI markers of cerebral small vessel disease: The Maastricht Study. GeroScience, 44(1), 147-157.

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Measuring Glomerular Filtration Rate

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We measured iohexol in serum by a validated liquid chromatography tandem mass spectroscopy (LC-MS/MS) method from four serial blood samples collected at 10, 30, 120, and 300 minutes post-iohexol injection with the clearance calculated using the concentration of iohexol as a function of time in two curves (fast and slow plasma disappearance).^{9 (link)} Serum creatinine was measured using an IDMS-traceable enzymatic method on the Roche Cobas 6000, following the FDA cleared procedure for Roche/Hitachi Cobas C systems. Blood urea nitrogen (BUN) and cystatin C were analyzed in serum on the Roche Cobas 6000, following the FDA cleared procedures for Roche/Hitachi Cobas C systems. The cystatin C method on the Roche Cobas 6000 employs an automated particle-enhanced immunoturbidimetric assay (PETIA).

Deng F., Finer G., Haymond S., Brooks E, & Langman C.B. (2014). Applicability of Estimating GFR Equations in Pediatric Patients: Comparison to a Measured GFR by Iohexol Clearance. Translational research : the journal of laboratory and clinical medicine, 165(3), 437-445.

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Serum Biomarker Measurements in CCTA

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Serum samples were collected just before CCTA, processed within 2 h and directly stored at − 80 °C until analysis. Total cholesterol (CV 2.0%), triglycerides (CV 2.5%), high-density (CV 3.0%) and low-density lipoprotein concentrations were measured as previously described (Cobas 6000, Roche Diagnostics) [28 (link)]. Serum creatinine (CV 2.5%) and cystatin C concentrations were measured in a fresh aliquot (Cobas 6000; Roche Diagnostics). Creatinine concentrations were assessed using the enzymatic method (Cobas 6000, Roche Diagnostics). Cystatin C was measured using a new particle-enhanced turbidimetric assay (Gentian AS), which was standardized against the certified ERM-DA471/IFCC cystatin C reference material [30 (link)]. Glomerular filtration rate was estimated by the Chronic Kidney Disease Epidemiology Collaboration equations using serum creatinine and cystatin C concentrations [31 (link)].

de Wit-Verheggen V.H., Altintas S., Spee R.J., Mihl C., van Kuijk S.M., Wildberger J.E., Schrauwen-Hinderling V.B., Kietselaer B.L, & van de Weijer T. (2020). Pericardial fat and its influence on cardiac diastolic function. Cardiovascular Diabetology, 19, 129.

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Serum Biomarker Quantification Protocol

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Fasting whole blood and serum samples were collected and processed for further analysis. Separated serum was stored at −70 °C. Plasma glucose was measured by enzymatic hexokinase method (Cobas 6000, Roche Diagnostics). Glycated hemoglobin (HbA1c) was assessed by turbidimetric inhibition immunoassay (Cobas 6000, Roche Diagnostics). This method is National Glycohemoglobin Standardization Programme (NGSP) certified according to Roche Diagnostics. Total cholesterol, HDL-c and triglycerides (TG) were estimated by enzymatic colorimetric methods (Cobas 6000, Roche Diagnostics). Insulin was determined by a microparticle enzyme immunoassay (Axsym, Abbott). C-reactive protein (CRP) was measured by a high-sensitivity CRP assay, based on the highly sensitive Near Infrared Particle Immunoassay rate methodology (Immageâ Immunochemistry System; Beckman Coulter).
Levels of HMW-adiponectin were measured in duplicate on serum by a sandwich ELISA (DRG® Adiponectin Human (HMW) ELISA). The assay is specific for HMW-adiponectin and has no interference from medium or low molecular weight adiponectin. The limit of sensitivity of the assay is 0.5 μg/mL and the appropriate range is 1.56–200 μg/mL. Serum samples were stored and are stable at −70 °C.

Zemlin A.E., Matsha T.E., Kengne A.P., Hon G, & Erasmus R.T. (2016). High Molecular Weight Adiponectin Levels are Neither Influenced by Adiponectin Polymorphisms Nor Associated with Insulin Resistance in Mixed-ancestry Hyperglycemic Subjects from South Africa. Journal of Medical Biochemistry, 35(4), 416-427.

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Lipid Profile and Insulin Sensitivity Assessment

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For lipid profile assessment, total cholesterol levels were quantified by an enzymatic colorimetric test, high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) levels by a homogeneous enzymatic colorimetric test, and triacylglycerol concentrations by an enzymatic colorimetric test (Cobas 6000, Roche Diagnostics, Mannheim, Germany).
To assess peripheral insulin sensitivity, the homeostasis model of insulin resistance HOMA-R was calculated, and to assess insulin secretion, the homeostasis model of beta cell function HOMA-F was calculated (Matthews et al. 1985). For these calculations, blood glucose levels were measured using an enzymatic reference method with hexokinase, insulin and C peptide by ECLIA (Cobas 6000, Roche Diagnostics, Mannheim, Germany). Glycated haemoglobin (HbA1c) was assayed by a turbidimetric inhibition method (Cobas 6000, Roche Diagnostics, Mannheim, Germany).

Vacínová G., Vejražkova D., Rusina R., Holmerová I., Vaňková H., Jarolímová E., Včelák J., Bendlová B, & Vaňková M. (2020). Regulated upon activation, normal T cell expressed and secreted (RANTES) levels in the peripheral blood of patients with Alzheimer’s disease. Neural Regeneration Research, 16(4), 796-800.

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Comprehensive Metabolic Profiling of Fasting Samples

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After 12 hrs of fasting, blood samples were collected by venipuncture, and urine samples were collected at the same time. All samples were shipped to the testing center of the Third Affiliated Hospital of Southern Medical University and analyzed within 3 hrs.
Insulin was determined by electrochemiluminescence (Roche cobas e601, Roche). Fasting blood glucose was measured using standard enzymatic methods. CRP (Roche cobas 6000, Orion) was measured by the immunoturbidimetric assay. The blood lipid test method was the colorimetric method (Roche cobas 6000). Serum creatinine was assessed using the modified Jaffe’s method (Roche cobas 6000, Roche). SUA levels were measured by colorimetry (Roche Diagnostics, Mannheim, Germany). The ACR value was calculated by measuring the albumin and creatinine concentrations of random urine samples.

Wang X., Chen H., Shao X., Xiong C., Hong G., Chen J., Li X., You X., Gao P., Chen Y., Zou Z., Ning J., Xiao H., Zou H, & Wei L. (2020). Association of Lipid Parameters with the Risk of Chronic Kidney Disease: A Longitudinal Study Based on Populations in Southern China. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 663-670.

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Urinary Albumin Excretion Assessment

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Two 24-h urine collections were used to assess urinary albumin excretion. Urinary albumin concentration was measured with a standard immunoturbidimetric assay by an automatic analyzer (owing to a change of supplier by the Beckman Synchron LX20, Beckman Coulter Inc.; and the Roche Cobas 6000, F. Hoffmann-La Roche) and multiplied by collection volume to obtain the 24-h urinary albumin excretion. A urinary albumin concentration below the detection limit of the assay (2 mg/L for the Beckman Synchron LX20 and 3 mg/L for the Roche Cobas 6000) was set at 1.5 mg/L before multiplying by collection volume. Only urine collections with a collection time between 20 h and 28 h were considered valid. If needed, urinary albumin excretion was extrapolated to a 24-h excretion. Albuminuria is seen as a risk marker for generalized endothelial dysfunction (29 (link)).

Linkens A.M., Houben A.J., Kroon A.A., Schram M.T., Berendschot T.T., Webers C.A., van Greevenbroek M., Henry R.M., de Galan B., Stehouwer C.D., Eussen S.J, & Schalkwijk C.G. (2021). Habitual intake of dietary advanced glycation end products is not associated with generalized microvascular function—the Maastricht Study. The American Journal of Clinical Nutrition, 115(2), 444-455.

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