Anti-phospho-p38 MAPK (Thr180/Tyr182) (9211S, Cell Signaling Technology), anti-p38α (sc-535, Santa Cruz), anti-Lamin B1(66095-1-Ig, Proteintech), anti-phospho-ERK(sc-81492, Santa Cruz), anti-phospho-JNK(sc-6254, Santa Cruz), anti-GAPDH (M20006; Abmart), anti-gag (ab63917, Abcam), anti-p24 (mouse ascites antibody), Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (Jackson Immunoresearch), Rhodamine(TRITC) AffiniPure Goat Anti-Rabbit IgG (Jackson Immunoresearch). α7 nicotinic acetylcholine receptor agonist: GTS-21 (ab120560, Abcam), DUSP1 and DUSP6 inhibitor: BCI (T10486, Topscience), ROS scavenger: N-Acetyl-L-methionine (T8059, Topscience).
Anti lamin b1 66095 1 ig
Manufactured by Proteintech
Sourced in United States
Anti-lamin B1 (66095-1-Ig) is a primary antibody that recognizes the lamin B1 protein. Lamin B1 is a structural protein that is a component of the nuclear lamina, a network of proteins that provide mechanical support and organization to the cell nucleus. This antibody can be used in various laboratory techniques, such as Western blotting and immunohistochemistry, to detect and study the lamin B1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Ab120560, by Abcam (1 mentions)
Anti phospho p38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions)
Anti gapdh m20006, by Abmart (1 mentions)
Alexa fluor 488 affinipure goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Rhodamine tritc affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Gts 21, by Abcam (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
Anti collageni ab34710, by Abcam (1 mentions)
Anti nrf2 gtx103322, by GeneTex (1 mentions)
Anti gapdh 60004 1g, by Proteintech (1 mentions)
Anti keap1 10503 2 ap, by Proteintech (1 mentions)
Protein assay, by Bio-Rad (1 mentions)
Anti n cadherin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Hrp conjugated goat anti rabbit igg sa00001 2, by Proteintech (1 mentions)
Hrp conjugated goat anti mouse igg sa00001 1, by Proteintech (1 mentions)
Ab179467, by Abcam (1 mentions)
Ab30930, by Abcam (1 mentions)
Anti gapdh 60004 1 ig, by Proteintech (1 mentions)
Ab124689, by Abcam (1 mentions)
4 protocols using anti lamin b1 66095 1 ig
1
Investigating MAPK Signaling in Virus Infection
2
Antibody Panel for Protein Analysis
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The following primary antibodies were used in this study: the anti-LC3B (sc-271625) was purchased from Santa Cruz Biotechnology; the anti-Collagen IV (ab6586), anti-Fibronectin (ab2413), anti-LC3β (ab192890), and anti-Collagen I (ab34710) were purchased from Abcam; the anti-Interleukin-1β (16806-1-AP), anti-CKAP4 (16686-1-AP), anti-Calnexin (10427-2-AP), anti-Interleukin-18 (60070-1-IG), anti-β-actin (60008-1-IG), anti-PACS-2 (19508-1-AP), anti-TFEB (13372-1-AP), anti-GAPDH (60004-1-IG) and anti-Lamin B1 (66095-1-IG) were purchased from Proteintech; the anti-FAM134B (83414 S) was purchased from Cell Signaling Technology; and anti-Flag (F1804) was purchased from Sigma-Aldrich.
3
Cell Lysis and Protein Extraction
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Cells were washed with PBS and harvested with RIPA lysis buffer (50 mM Tris (pH 7.5), 150 mM NaCl, 1mM EDTA, 1% NP-40, 0.1% SDS) supplemented with protease and phosphatase inhibitors (1 mM PMSF, 10 μg/mL Leupeptin, 50 μg/mL TLCK, 50 μg/mL TPCK, 1 μg/mL Aprotinin, 1 mM NaF, 5 mM NaPPi, and 10 mM Na3VO4). The cell lysates were cleared via 15 min spins at 13,000 rpm, 4 °C. Protein concentrations were determined by Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA). The following antibodies were used: anti-NRF2 (GTX103322, GeneTex, Irvine, CA, USA), anti-GAPDH (60004-1g, Proteintech, Rosemont, IL, USA), anti-KEAP1 (10503-2-AP, Proteintech, Rosemont, IL, USA), anti-lamin B1 (66095-1-Ig, Proteintech, Rosemont, IL, USA).
4
Western Blotting and ChIP Antibody Panel
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The following antibodies were used for western blotting or chromatin-immunoprecipitation (ChIP). Anti-NDRG1 (ab124689, Abcam), anti-ACTA1 (ab179467, Abcam), anti-c-Jun (ab32137, Abcam), phalloidin-iFluor 488 (ab176753, Abcam), and anti-hnRNPR (ab30930, Abcam) were purchased from Abcam. The anti-hnRNPR antibody (GTX106526, GeneTex) was purchased from GeneTex. Anti-N-cadherin (13116, Cell Signaling Technology), anti-E-cadherin (3195, Cell Signaling Technology), anti-vimentin (5741, Cell Signaling Technology), and anti-Snail (3879, Cell Signaling Technology) antibodies were purchased from Cell Signaling Technology. Anti-GAPDH (60004-1-Ig, Proteintech) and anti-lamin B1 (66095-1-Ig, Proteintech) antibodies, HRP-conjugated goat anti-mouse IgG (SA00001-1, Proteintech), and HRP-conjugated goat anti-rabbit IgG (SA00001-2, Proteintech) were purchased from Proteintech.
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