Somatostatin sst

Manufactured by Merck Group

Sourced in United States

Somatostatin (SST) is a naturally occurring peptide hormone found in the human body. It plays a role in regulating the secretion of various other hormones, including growth hormone, thyroid-stimulating hormone, and insulin. Somatostatin is utilized in laboratory settings for research and experimentation purposes.

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Somatostatin (31 citations)

3 protocols using somatostatin sst

Caco-2 Cells: IFN-γ and Somatostatin Effects

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The human colon cancer cell line Caco-2 was purchased from BeNa Culture Collection (BNCC102170; BNCC; China). Cells were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM; Gibco; USA) containing 10% fetal bovine serum (FBS; Gibco; USA), 100 U/mL penicillin and 0.1 mg/mL streptomycin in a humidified incubator (37°C and 5% CO2).
For IFN-γ treatment, Caco-2 cells were incubated in DMEM and 100 U/mL IFN-γ (Sigma–Aldrich; USA) for 48 h. In addition, somatostatin (SST; Sigma–Aldrich; USA) was dissolved in DMEM and added to the culture medium of Caco-2 cells at an experimental concentration of 1 μM 2 h before IFN-γ treatment.
For cell transfection, Tollip siRNAs (siTollip) were constructed by Guangzhou RiboBio Biotechnology Co., Ltd. (Guangzhou; China) and transfected with Lipofectamine® 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.; USA) following the instructions. The transfection efficiency was verified by Western blotting 24 h after transfection.

Tian Y., Shu R., Lei Y., Xu Y., Zhang X, & Luo H. (2022). Somatostatin attenuates intestinal epithelial barrier injury during acute intestinal ischemia–reperfusion through Tollip/Myeloiddifferentiationfactor 88/Nuclear factor kappa-B signaling. Bioengineered, 13(3), 5005-5020.

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Neurochemical Solutions for Behavioral Research

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Clozapine-n-oxide (CNO; 1 mg/kg in experiments 1 & 2, 2mg/kg in experiment 3; RTI International, Research Triangle Park, NC) was dissolved with 1% Dimethylsulfoxide (DMSO; Sigma Life Sciences, St. Louis, MO) in 0.9% NaCl (Baxter Laboratories, Deerfield, IL). Corticotropin releasing factor (CRF; Tocris Bioscience, Minneapolis, MN), somatostatin (SST; Sigma Life Sciences, St. Louis, MO), and neuropeptide Y (NPY; AnaSpec, Fremont, CA) were each suspended in artificial cerebrospinal fluid (aCSF). Antalarmin hydrochloride (Tocris Bioscience), a selective CRFR1 antagonist, was dissolved in 30% DMSO diluted in Dulbecco’s phosphate buffered saline (Thermo Fisher Scientific, Waltham, MA) to a final concentration of 1.0 ug/uL. Solutions were made fresh daily. Ethanol (Decon Labs, King of Prussia, PA) was diluted to 20% (v/v) in tap water for use in DID. Saccharin (Sigma Life Sciences) was dissolved in tap water to a concentration of 9.2 mM.

Borrego M.B., Grigsby K.B., Townsley K.G., Chan A., Firsick E.F., Tran A, & Ozburn A.R. (2021). Central nucleus of the amygdala projections onto the nucleus accumbens core regulate binge-like alcohol drinking in a CRF-dependent manner. Neuropharmacology, 203, 108874.

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Functionalized Gold Nanoparticle Synthesis

Cited 3 times

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Tetrachloroauric (III) trihydrate (HAuCl₄·3H₂O: 99.9%), sodium citrate tribasic dehydrate (≥99%) and hydroquinone (≥99%) were purchased from Sigma-Aldrich. Heterobifunctional polyethylene glycol OPSS-PEG-SVA (3.4 kDa, OPSS-PEG-SVA-3400) were purchased from Laysan Bio Inc. Poly(ethylene glycol) 2-mercaptoethyl ether acetic acid (Thiol-PEG-Carboxyl, 600 Da, PG2-CATH-600) were purchased from Nanocs Inc. Fluorescein isothiocyanate–dextran (FITC-dextran) and WST-1 cell proliferation reagent (05015944001) was purchased from Sigma-Aldrich. Propidium iodide (PI) is purchased from Cayman chemical (14289). Mouse IgG isotype control (Ctrl Ab, 31901) was purchased from Invitrogen. Anti-PAR2 antibody (MAB3949) was purchased from R&D systems. Anti-JAM-A antibody BV16 was kindly provided by Dr. Elisabetta Dejana’s lab.^{32 (link)} Human cerebral microvessel endothelial cells (hCMEC/D3) were purchased from EMD Millipore. HEK293 cell with Ca²⁺ indicator TN-XXL was kindly provided by Dr. Paul Slesinger’s lab.^{44 (link)} PAR2 agonist 2-aminothiazol-4-yl-LIGRL-NH₂ (2AT) was provided by Dr. Theodore Price’s lab.^{40 (link)} Somatostatin (SST) was purchased from Sigma-Aldrich (S9129). Pierce modified lowery protein assay kit was obtained from ThermoFisher Scientific (23240).

Kang P., Li X., Liu Y., Shiers S.I., Xiong H., Giannotta M., Dejana E., Price T.J., Randrianalisoa J., Nielsen S.O, & Qin Z. (2019). Transient Photo-Inactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia. ACS nano, 13(11), 12487-12499.

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