Gscu300cc 50

Copper TEM grids with a formvar-carbon support film (GSCU300CC-50, ProSciTech, Qld, Australia) were glow discharged for 60 s in an Emitech k950x with k350 attachment. GS-1 was diluted in water to 0.01 mM, 0.1 mM, 0.8 mM, and 1 mM. Four microlitre drops of GS-1 suspension was pipetted onto each grid, allowed to adsorb for at least 30 s and blotted with filter paper. Two drops of 2% uranyl acetate were used to negatively stain the particles blotting after 10 s each. Grids were then allowed to dry before imaging. Grids were imaged using a Joel JEM-2100 (JEOL (Australasia) Pty Ltd., Brookvale, Australia) transmission electron microscope equipped with a Gatan Orius SC 200 CCD camera (Scitek Australia).

Copper TEM grids with a formvar-carbon support film (GSCU300CC-50, ProSciTech, Qld, Australia) were glow discharged for 60 seconds in an Emitech k950x with k350 attachment. Two 5 µl drops of EV suspension were pipetted onto each grid, allowed to adsorb for at least 30 seconds and then blotted with filter paper. Two drops of 2% uranyl acetate were used to negatively stain the particle blottings after 10 seconds each time. Grids were then allowed to dry before imaging. Grids were imaged using a Joel JEM-2100 (JEOL Australasia Pty Ltd) transmission electron microscope equipped with a Gatan Orius SC 200 CCD camera (Scitek Australia).

Copper TEM grids featuring a formvar–carbon support film (GSCU300CC-50, ProSciTech, Kirwan, QLD, Australia) underwent a 60 s glow discharge in an Emitech k950x with a k350 attachment. Dilutions of 0.003 μM, 0.03 μM, 0.30 μM, and 3.00 μM of GS-9 were made up in water. Each grid received four-microliter drops of each GS-9 concentration, then allowed to adsorb for at least 30 s before subsequently being blotted with filter paper. Negative staining of the particles was performed using two drops of 2% uranyl acetate, with blotting after 10 s for each drop. The grids were then left to dry before the imaging process. Imaging was conducted using a Joel JEM-2100 transmission electron microscope equipped with an AMD NanoSprint II (Newspec, Myrtle Bank, SA, Australia), provided by JEOL Australasia (Brookvale, NSW, Australia).