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5 protocols using pelco conductive silver paint

1

Skin Specimen Inoculation and Visualization

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Using the prevention strategy as outlined in Section 2.4, skin specimens were first treated with ABIL and Eudragit, followed by inoculation with microconidia. These samples were then incubated for 24 h at 30 °C and visualized with scanning electron cryo-microscopy. For this, the skin specimens were directly mounted on SEM pin stubs with PELCO conductive silver paint (Ted Pella, Redding, CA, USA) and frozen immediately in liquid nitrogen. The specimens were then coated in a sputter coater and imaged by a Quanta 200 FEG SEM (FEI, Hillsboro, OR, USA).
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2

Custom-Made Microdrive Construction for Neurophysiology

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Microdrives were constructed as described previously [39 (link), 96 (link)]. Briefly, custom-made reusable 16-channel or 32-channel microdrives (Axona, UK) were outfitted with 4 to 8 tetrodes consisting of twisted, 25-μm-thick platinum-iridium wires (California Wires, USA) funneled through a 23-gauge stainless steel inner cannula. A 19-gauge protective, stainless steel outer cannula was slipped over the inner cannula and secured to the microdrive body via modeling clay. Individual electrodes were wrapped tightly around the exposed wires of the microdrive and coated with a layer of Pelco conductive silver paint (Ted Pella, Inc., USA) prior to sealing of the microdrive body with liquid electrical tape (Gardner Bender, USA). Several hours prior to surgery, the tetrodes were cut to an appropriate length and electroplated with a platinum/gold solution until the impedances dropped within a range of 150–200 Kohms.
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3

Textile-integrated Thermoelectric Generator

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The raw CNT material used for the device was produced by Meijo Nano Carbon Co., and the average diameter was determined to be 1.8 nm with an average of 1.5 walls. The aspect ratio was 4100. CNT fibers were produced using the same method but using a 2 wt% solution of CNTs in CSA. The fibers were washed in water, dried in oven overnight to stabilize electrical properties for textile applications. Twenty-one CNT filaments were plied together to create a sewing thread, and the average diameter of the thread was 190 μm.
The CNT thread and stainless steel thread (NGW-1pc Conductive Stainless Steel Sewing Thread), were sewn onto the fabric in 4 cm lengths using a commercial sewing machine (Singer 2277 Tradition). Silver paste (PELCOⓇ Conductive Silver Paint, Ted Pella, Inc.) was applied at junctions for better electrical connections. Fifteen CNT threads were connected thermally in parallel and electrically in series to create one TEG unit, and the four units were connected in series as shown in Supplementary Figs. 19 and 20. The resistance of the entire TEG was ~300 Ω. The cold side was at room temperature, and the hot side was placed on the hotplates.
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4

Fabrication of Conductive Electrochemical Sensors

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Potassium nitrate (99%), agar (Fisher BioReagents™), potassium ferrocyanide trihydrate (98.5%, certified ACS), sulfuric acid (93–98%, trace metal grade) and potassium ferricyanide (99%, certified ACS) were purchased from Fisher Scientific (Ottawa, Ontario). Isopropyl alcohol (99%), sodium perchlorate monohydrate (98%), chloroauric acid (99.995%) and copper(ii) sulfate (99.999%, trace metal basis) were purchased from Sigma-Aldrich (Oakville, Ontario). Potassium chloride (99%) was sourced from Millipore Sigma (Burlington, Massachusetts). Protopasta PLA Conductive filament was obtained from HatchBox (Pomona, California). Multi3D Electrifi Conductive filament was purchased from Multi3D (Middlesex, North Carolina). Gorilla Epoxy™ was purchased from Gorilla Glue Company (Cincinnati, Ohio). Mill-Q (MQ) water (>18.2 MΩ) was used to prepare aqueous solutions. PELCO® conductive silver paint was obtained from TED Pella Inc. (Redding, California).
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5

Fabrication and Characterization of LIG Sensor

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The standard stock solution of Al (III) 1000 mg L−1 was purchased from MOL LABS. The other reagents, including Pb (II), Cd (II) and Cu (II) (1000 mg L−1) standards, were purchased from SIGMA (ref: 41,318; 36,379). A stock Bi (III) solution 0.2 mol L−1 was prepared by adding Bi(NO3)3·5H20 into acetate solution (sodium acetate + acetic acid 0.1 mol L−1) at pH 3.70. Working solutions were prepared in a concentration range from 1 to 300 ppm by diluting the stocks in acetate solution at 0.1 mol L−1 (pH 3.70) containing the Bi (III) solution at 0.1 mol L−1. The Ferri solution used for electrochemical characterization consisted of a solution of potassium hexacyanoferrate (III) [K3Fe (CN)6] 2 mmol L−1 (Alfa Aesar, ref: 33,357) with H2SO4 0.18 mol L−1. All solutions were prepared with ultrapure water obtained from Thermo Fisher Scientific Barnstead SMART2PURE water system, with conductivity 18.2 Ω. For LIG fabrication, we used Polyester Diagnostic Tape (PT) (3 M™, ref: 9964) as a substrate, 50 mm thickness Polyimide (PI) film (Shijiazhuang Dadao Packaging Materials Co., Ltd), PELCO® Conductive Silver Paint (Ted Pella Inc, ref: 16,062) for all electrode contact points, and Silver/Chloride-silver (Ag/AgCl) ink (DuPont™, ref: 5874) for the pseudo-reference electrode.
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