The largest database of trusted experimental protocols

5 protocols using o coumaric acid

1

Organic Black Quinoa Phytochemical Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dehulled seeds of organic black quinoa (Chenopodium quinoa Willd.), labelled as originating from Bolivia (South America) and packed in a protective atmosphere, were purchased in a retailer specialized in organic and biodynamic products in Italy. Proximate composition per 100 g was, as reported in the nutrition label: 380 kcal energy; 6.2 g fat, of which 0.7 g saturates; 65 g carbohydrates, of which 4.0 g sugars; 9.0 g fibre; 12 g protein; and 0.01 g salt.
Folin–Ciocalteu’s Reagent, methanol, ethanol, acetone, acetic acid and acetonitrile were purchased from Carlo Erba Reagents (Milan, Italy).
Caffeic acid, (+)-catechin, o-coumaric acid, p-coumaric acid, (−)-epicatechin, 3,4-dihydroxycinnamic acid, t-ferulic acid, gallic acid, gentisic acid, 4-hydroxybenzoic acid, protocatechuic acid, rutin, sinapic acid, syringic acid, vanillic acid, and vanillin were purchased from Extrasynthèse (Geney, France) and Sigma-Aldrich (St. Louis, MO, USA).
HPLC grade solvents and water purified by a Milli-Q system (Millipore Corp., Billerica, MA, USA) were used in HPLC analysis.
+ Open protocol
+ Expand
2

Identification and Quantification of Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetonitrile (hypergrade for LC–MS), formic acid (for LC-MS) and methanol (analytical grade) were purchased from Merck (Merck, Bulgaria). The authentic standards used for compound identification were obtained from Extrasynthese (Genay, France) for protocatechuic, syringic, vanillic gentisic, p-coumaric, m-coumaric, o-coumaric acid, quercetin, apigenin, luteolin, apigenin 7-O-glucoside, kaempferol 3-O-glucoside, isorhamnetin 3-O-glucoside, luteolin 7-O-glucoside and rutin, and kaempferol 3-O-rutinoside, homoorientin, isoquercitrin, hyperoside, nepetin. Caffeic acid, neoChlorogenic acid, 3,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, jaceosidin and hispidulin were supplied from Phytolab (Vestenbergsgreuth, Germany). Chlorogenic acid, isorhamnetin and cirsimaritin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
+ Open protocol
+ Expand
3

Analytical Protocols for Phytochemicals

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetonitrile (hypergrade for LC-MS), formic acid (for LC-MS) and methanol (analytical grade) were purchased from Merck, Sofia, Bulgaria. The authentic standards used for compound identification were obtained from Extrasynthese (Genay, France) for protocatechuic, p-hydroxyphenylacetic, vanillic, gentisic, ferulic, p-coumaric, o-coumaric acid, rutin, hyperoside, luteolin 7-O-rutinoside, luteolin 7-O-glucoside, isoquercitrin, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, kaempferol 3-O-glucoside, isorhamnetin 3-O-glucoside, apigenin 7-O-glucoside, quercetin, apigenin, luteolin, chrysoeriolq eupatilin and genkwanin. Caffeic, 3,4-dicaffeoylquinic, 3,5-dicaffeoylquinic 1,5-dicaffeoylquinic, 4,5-dicaffeoylquinic and 3,4,5- tricaffeoylquinic acids were supplied by Phytolab (Vestenbergsgreuth, Germany). Chlorogenic acid and isorhamnetin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Diclofenac, silymarin, collagenase, 1-chloro-2,4-dinitrobenzene, beta-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH), ethylenediaminetetraacetic acid (EDTA), bovine serum albumin (fraction V) and 2,2′-dinitro-5,5′dithiodibenzoic acid (DTNB) were obtained from Merck (Darmstadt, Germany). Reduced glutathione (GSH), oxidized glutathione (GSSG), glutathionereductase (GR) and cumene hydroperoxide were purchased from Sigma Chemical Co. (Taufkirchen, Germany).
+ Open protocol
+ Expand
4

HPLC Analysis of Fermented Olive Mill Wastewater

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HPLC analyses of fermented OMWW samples were performed by directly injecting the filtered samples (0.45 µm PTFE filters, Merck, Darmstadt, Germany) into the HPLC chromatographic system, i.e., Waters Alliance 2695 HPLC liquid chromatography equipped with a Waters 996 photodiode array (PDA) detector set at 280 nm and managed through the Waters Empower software (Waters Corporation, Milford, MA, USA). The column used was a Luna C18 (250 mm × 4.6 mm i.d., 5 m, 100 Å; Phenomenex, Torrance, CA, USA) maintained in an oven at 40 °C. A flow rate of 1 mL/min was used. Chromatographic separation was performed according to Romeo et al. [11 (link)]. The internal standard (I.S.), 50 mM pure gallic acid (Fluka, Buchs Switzerland), was used to quantify the phenolic compounds. The identification of phenolic compounds was obtained by comparing the peak retention time with those of pure standards of tyrosol (TYR), oleuropein (OLE), hydroxytyrosol (HT) chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, verbascoside, luteolin-7-o-glucoside, o-coumaric acid, rutin, oleuropein, apigenin-7-o-glucoside, luteolin-4-glucoside, quercetin, luteolin, apigenin (Extrasynthese, Genay, France). All analyses were performed in triplicate for each sample.
+ Open protocol
+ Expand
5

HPLC-MS Analysis of Polyphenolic Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical standards of 3,4-dihydroxybenzoic acid (>98.2%), acacetin (>98.7%), apigenin (>99%), caffeic acid (>98.5%), catechin (>99%), chlorogenic acid (>99%), chrysin (>99%), daidzein (>99%), galangin (>98.5%), gallic acid (>95.5%), genistein (>99%), (-)-epicatechin (>90.3%), folic acid (>91.2%), formononetin (>99%), isovitexin (>99%), luteolin (>99.9%), myricetin (>98%), o-coumaric acid (>99.7%), p-coumaric acid (>99.6%), p-hydroxybenzoic acid (>99.9%), pantothenic acid (>98.6%), phenylacetic acid (>99.7%), rhamnetin (>99%), rutin trihydrate (>94%), quercetin (>98%), sinapic acid (>96%), syringic acid (>98.5%), trans-ferulic acid (>99.8%), vanillic acid (>98.2%) were purchased from Extrasynthese (Lyon, France) or Sigma-Aldrich (St. Louis, MO, USA). The standard of (-)-cis, trans-abscisic acid (>99.9%) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and kaempferol (>97%) was purchased from ChromaDex (Santa Ana, CA, USA). HPLC-MS grade acetonitrile (MeCN) and dimethyl sulfoxide (DMSO) were purchased from Merck (Darmstadt, Germany), while formic acid (FA), hydrochloric acid (HCl), and sodium chloride (NaCl) were purchased from Sigma-Aldrich.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!