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Eclipse lv100pol microscope

Manufactured by Nikon

The Eclipse LV100POL is a polarizing microscope designed for materials analysis and research. It features a sturdy and stable construction, providing reliable performance. The microscope is equipped with a range of objective lenses and observation methods, enabling comprehensive sample examination.

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13 protocols using eclipse lv100pol microscope

1

Optical Properties of Monodomain LCE 1

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Polarizing optical microscopy (POM) images were acquired on a Nikon ECLIPSE LV100POL microscope equipped with a Nikon Digital Sight DS-U3 camera, purchased from Beijing Ruich Allway Instrument Technology Co. Ltd. POM was used to observe the optical properties of monodomain LCE 1.
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2

Coccolithophore Mass and Length Estimation

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For determination of coccolith mass and length estimates of the sediment trap samples, the glass slide preparations of raw sediment trap material used for coccosphere identification were employed. A total of 2355 fields of view (FOV) were imaged using a Nikon Eclipse LV100 POL microscope equipped with circular polarisation and a Nikon DS-Fi1 8-bit colour digital camera. A detailed description of the circular polarization microscope set-up applied in this study can be found in40 (link) while further details of the methodology used here can be found in Supplement 4.
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3

Palynological Analysis Procedure

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Palynological analysis was carried out on the basis of procedures put forward by Moore et al. [34] , Lentfer et al. [35] and Horrocks [36] (link). 50 g dried powder samples were processed with HCl, KOH (10%, 100 ml), ZnCl2 (2.0 g/ml in density, 30 ml), acetic acid (100 ml), KI/IH (2.0 g/ml in density, 5 ml) and acetolysis mixture (1 ml concentrated H2SO4, 9 ml acetic anhydride). A tablet of Lycopodium marker (18583 grains) was added to each sample. The pollen samples were spread uniformly on glass slides and at least 500 pollen grains, excluding aquatic pollen and spores, identificated and counted using a Nikon Eclipse LV100POL microscope. Identification was aided by the use of reference materials collected by the Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences and published keys [37] .
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4

Neurite Length Measurement Protocol

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The samples were transferred onto a glass slide that had PBS as the mounting medium after 4 days of exposure in differentiation medium. A cover glass was mounted onto the slide. The fluorescence images were collected under the Nikon Eclipse LV100 POL microscope. For each group, 3 to 4 samples were prepared and for each sample, 10 to 12 images on different spots of the film were taken. All the images were loaded into FIJI software. The length of neurites was measured by drawing a line from the neurite end to the junction between neurite cell bodies using FIJI software.
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5

NMR, Thermal, and Structural Characterization

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NMR spectra were recorded on a Bruker Ascend 400 (400.13 MHz for 1H and 100.60 MHz for 13C) spectrometer. 1H chemical shifts were referenced to the proton resonance signal resulting from the protic residue in the deuterated solvent. 13C chemical shifts were referenced to the carbon resonance signal resulting from the deuterated solvent. Thermogravimetric analysis was carried out on a TGA Q5000 instrument (TA Instruments) under a nitrogen atmosphere. Elemental analysis was performed at the Organic Chemistry Research Center, Sogang University (Seoul, Korea) using an EA1112 instrument (CE Instruments, Italy). Powder XRD spectra were obtained with a Bruker D8 Focus diffractometer (40 kV, 30 mA, step size = 0.02°). Optical microscopic images were collected on a Nikon Eclipse LV100POL microscope equipped with a DS-Fi1 CCD camera.
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6

Characterization of Photochromic Compounds

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Differential scanning calorimetry (DSC) was performed on a NETZSCH DSC 3500 Sirius calorimeter. The sample was enclosed into the aluminum pan, and then DSC scans were conducted under dry nitrogen gas. The heating and cooling rates were 10 °C min−1. Powder X-ray diffraction (PXRD) was measured on a Rigaku RINT 2500 diffractometer.
Optical microscopic data were captured on a Nikon ECLIPSE LV100POL microscope with a heat stage (Linkam, THMS600). UV (365 nm) and visible light (525 nm) irradiation was conducted with an ASAHI SPECTRA CL-1501 controller and LED lamp. UV-vis absorption spectra were recorded with a JASCO V-750 spectrophotometer, with sample concentration of 1.0 × 10−4 mol L−1 (CHCl3 solution).
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7

Optical and SEM Microscopy Protocol

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Optical microscopy studies used a Nikon Eclipse LV100POL microscope with both transmitted and reflected-light capabilities, a maximum of 1,000× magnification and a SPOT Insight 4-MP CCD colour digital camera assembly. SEM analyses were also conducted at the University of Rochester using a Zeiss Auriga SEM. For subsurface inclusions, we estimated depth using Electron Flight Simulator Version 3.1E software.
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8

Microscopic Magnetic Grain Analysis

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Optical microscopy studies (University of Rochester) used a Nikon Eclipse LV100POL microscope with both transmitted and reflected light capabilities, a maximum 1,000× magnification, and a Spot Insight 4MP CCD color digital camera assembly. MOKE analyses were performed at the Naval Research Laboratories. Grains were mounted on epoxy stubs and polished (first with alumina to 0.5 μ m followed by 0.05 μ m colloidal silica) to expose the grain interiors. The instrument employed (Quantum Designs NanoMOKE3) is capable of detecting micrometer to submicrometer magnetic features at depths on the order of 1 to 10 μm beneath the polished surface. Samples were exposed to a magnetic field ( 500 mT) and imaged using both polar (field applied normal to grain surface) and longitudinal (field applied parallel to grain surface) modes. Reflected laser light and Kerr effect images were collected simultaneously, allowing for the discrimination between optical and magneto-optical features.
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9

Petrographic Analysis of Carbonate Concretions

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Bone fragments partially enclosed in the carbonate concretion were selected for petrographic microfacies analyses. Ten uncovered, polished petrographic thin sections (30 μm thick) were analysed via an optical light microscope in transmitted and reflected light, using a Nikon eclipse LV100POL microscope equipped with a Nikon DSFil digital camera with NIS-Elements BR imaging software. Selected portions of the thin sections were etched in an aqueous solution of 1% HCl between 10 and 30 s, air dried and C-coated for scanning electron microscope observations and element analysis (scanning electron microscopy-energy-dispersive X-ray spectroscopy). The analyses were carried out with a JEOL JSM-6610 LV scanning electron microscope equipped with a AzTEC EDS for element identification. The operating conditions of the scanning electron microscopes were 5–20 keV accelerating voltage for imaging, and 15–20 keV for elemental analyses.
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10

Phytolith Extraction and Identification

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Following Lentfer and Boyd [38] and Pearsall [39] , each 5 g dried powder sample was processed with NaHCO3 (5%, 30 ml), HCl (10%, 30 ml), H2O2 (30%, 30 ml) and KI/CdI2 (2.3 g/ml in density). In order to remove particles less than 20 µm, the sample was sedimented by Stokes’ Law. Based on a total of at least 500 phytoliths, identification and counting were carried out after the sample was dried at room temperature. Identification was made under a Nikon Eclipse LV100POL microscope using the standard keys [10] , [39] , [40] –[43] and the reference collection of the Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences. The phytoliths were designated in accordance with the International Code for Phytolith Nomenclature [44] (link).
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