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Mouse insulin elisa kit

Manufactured by Thermo Fisher Scientific
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The Mouse Insulin ELISA Kit is a quantitative immunoassay designed for the measurement of insulin levels in mouse samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify insulin concentrations.

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Lab products found in correlation

Ethanol assay kit, by BioAssay Systems (2 mentions) Cholesterol assay kit, by Abcam (2 mentions) Hydroxyproline assay kit, by Merck Group (2 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (2 mentions) Mouse rat leptin quantikine elisa kit, by R&D Systems (2 mentions) Triglyceride assay kit, by Abcam (1 mentions) Accu chek, by Roche (1 mentions) Infinity triglyceride kit, by Thermo Fisher Scientific (1 mentions) Superose 6 10 300, by GE Healthcare (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Kta pure fplc unit, by GE Healthcare (1 mentions) Cholesterol, by Fujifilm (1 mentions) Hr series nefa hr, by Fujifilm (1 mentions) Standard assay kits, by Abcam (1 mentions) Cholesterol quantitation kit, by Merck Group (1 mentions) Mouse adiponectin elisa kit, by Thermo Fisher Scientific (1 mentions) Mouse tnf α elisa kit, by Merck Group (1 mentions) Streptozotocin (stz), by Thermo Fisher Scientific (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Standard assay kit, by Merck Group (1 mentions)

37 protocols using mouse insulin elisa kit

1

Evaluating Plasma Biomarkers in Male Subjects

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We measured male plasma alcohol concentrations using an Ethanol Assay Kit (catalog no. ECET100; BioAssay Systems, Hayward, CA, USA) according to manufacturer's protocol. We determined plasma insulin levels post-mortem using the Mouse Insulin ELISA kit (catalog no. EMINS; Thermo-Fisher, Waltham, MA, USA), according to the recommended protocol. We measured levels of IL1B, IL6, and TNFα using commercial ELISA assays (catalog no. KMC0061 and KMC0011; Thermo-Fisher, Waltham, MA, USA and catalog mo. Ab100689 and Ab100747; Abcam, Cambridge, MA, USA). To determine total cholesterol levels, we used the Total Cholesterol Assay Kit (catalog no. STA- 384; Cell Biolab, Inc, San Diego, CA USA), according to the recommended protocol. To compare the levels of low-density and high-density lipoproteins, we used a colorimetric Cholesterol Assay Kit (catalog no. ab65390; Abcam, Cambridge, MA, USA), according to the recommended protocol. We used the Triglyceride Assay Kit (catalog no. ab65336; Abcam, Cambridge, MA, USA) to contrast the abundance of hepatic triglycerides, according to the suggested protocol. To compare the levels of hydroxyproline, we used the Hydroxyproline Assay Kit (catalog no. MAK008; Millipore-Sigma, St. Louis, MO, USA), and followed the recommended protocol.
Chang R.C., Thomas K.N., Bedi Y.S, & Golding M.C. (2019). Programmed increases in LXRα induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Molecular Metabolism, 30, 161-172.
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2

Metabolic and Inflammatory Biomarkers Profiling

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Triglyceride and glucose concentrations were measured in blood with MultiCare strips (Biochemical Systems International, Italy). Plasma insulin was quantified with a mouse insulin ELISA kit (Thermo Fisher Scientific, MA) according to the instructions of the manufacturer. Circulating levels of inflammatory cytokines (TNF‐α, MCP‐1, IL‐6, IL‐1β, leptin, and adiponectin) were monitored in plasma with commercially available LUMINEX kits (Affymetrix, eBioscience, Austria) according to the instructions of the manufacturer.
All samples were analyzed in duplicate.
Setayesh T., Mišík M., Langie S.A., Godschalk R., Waldherr M., Bauer T., Leitner S., Bichler C., Prager G., Krupitza G., Haslberger A, & Knasmüller S. (2019). Impact of Weight Loss Strategies on Obesity‐Induced DNA Damage. Molecular Nutrition & Food Research, 63(17), 1900045.
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3

Evaluating PF Sprout Extract Efficacy

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To study the effectiveness of PF sprout extract at different doses, a blood glucometer (ACCU-CHEK, Roche Diagnostics, Mannheim, Germany) measured the fasting (12 hours) blood glucose weekly during the treatment period, and insulin levels were determined using the enzyme-linked immunosorbent assay (ELISA) with a mouse insulin ELISA kit (ThermoFisher Scientific, Waltham, MA, USA).
Kim D.H., Kim S.J., Yu K.Y., Jeong S.I, & Kim S.Y. (2018). Anti-hyperglycemic effects and signaling mechanism of Perilla frutescens sprout extract. Nutrition Research and Practice, 12(1), 20-28.
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4

Postprandial Plasma Insulin and TAG

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Postprandial state mice were anaesthetized with isoflurane and blood was collected from the right atrium. For fasting state mice, blood was collected from the tail. Collected blood was centrifuged at 2000 g at 20 °C for 7 minutes. After centrifugation, the plasma fractions were recovered and frozen in aliquots at −80 °C. Plasma insulin was measured by Mercodia Mouse Insulin Elisa kit and plasma TAG level was determined using the Thermo Scientific Infinity Triglyceride kit according to manufacturer’s instructions.
Ning F.C., Jensen N., Mi J., Lindström W., Balan M., Muhl L., Eriksson U., Nilsson I, & Nyqvist D. (2020). VEGF-B ablation in pancreatic β-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake. Scientific Reports, 10, 923.
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5

Comprehensive Plasma Biomarker Analysis

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Male plasma alcohol concentrations were measured using an Ethanol Assay Kit (catalog# ECET100; BioAssay Systems, Hayward, CA, USA) according to manufacturer’s protocol. Animals were euthanized by cervical dislocation, blood collected postmortem and plasma insulin levels determined using the Mouse Insulin ELISA kit (catalog# EMINS; Thermo-Fisher, Waltham, MA, USA), according to the recommended protocol. Levels of IL1B, IL6, INFg and TNFa were determined using commercial ELISA assays (catalog# KMC0061 and KMC0011; Thermo-Fisher, Waltham, MA, USA and catalog# Ab100689 and Ab100747; Abcam, Cambridge, MA, USA). Total cholesterol levels were determined using the Total Cholesterol Assay Kit (catalog# STA-384; Cell Biolab, Inc, San Diego, CA USA), according to the recommended protocol. The comparative levels of low-density and high-density lipoproteins were determined using a Cholesterol Assay Kit (catalog# ab65390; Abcam, Cambridge, MA, USA), according to the recommended protocol. The levels of hydroxyproline were determined using the Hydroxyproline Assay Kit (catalog# MAK008; Millipore-Sigma, St. Louis, MO, USA), following to the recommended protocol.
Chang R.C., Wang H., Bedi Y, & Golding M.C. (2019). Preconception paternal alcohol exposure exerts sex-specific effects on offspring growth and long-term metabolic programming. Epigenetics & Chromatin, 12, 9.
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6

Liver Lipid Extraction and Lipoprotein Analysis

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Livers were disrupted in homogenization buffer (10 mM Tris-HCl [Bioland], pH 7.4, 150 mM NaCl [Sigma-Aldrich], 1 mM EDTA [Bioland], and protease inhibitors [Thermo Fisher Scientific]) and lipids were extracted from 0.5–1 mg liver protein using the Folch method (26 (link)). Liver and plasma cholesterol and triglyceride were measured by colorimetric assay (cholesterol, WAKO; TG, Sekisui; glycerol, Cayman [biomol]; NEFA, Fujifilm, HR Series NEFA-HR). Plasma insulin was measured by Mouse Insulin ELISA Kit (Thermo Fisher Scientific). To resolve lipoprotein classes, plasma samples were injected into a Superose 6 10/300 (GE Healthcare Life Sciences) column attached to ÄKTA Pure FPLC unit (GE Healthcare) and fractions were collected for measurement of cholesterol by colorimetric assay (WAKO).
Xiao X., Kennelly J.P., Feng A.C., Cheng L., Romartinez-Alonso B., Bedard A., Gao Y., Cui L., Young S.G., Schwabe J.W, & Tontonoz P. (2024). Aster-B–dependent estradiol synthesis protects female mice from diet-induced obesity. The Journal of Clinical Investigation, 134(4), e173002.
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7

Serum Biomarkers of Metabolism

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Serum insulin, Lcn2, and Leptin were measured by the Mouse Insulin ELISA kit (Thermo Scientific, Frederick, MD), Mouse Lcn2 ELISA kit (Biolegend, San Diego, CA), and a Quantikine ELISA kit for mouse leptin (R&D Systems, Minneapolis, MN), respectively. Serum triglycerides and β-hydroxybutyrate were measured using the Stanbio LiquiColor® Triglyceride and β-hydroxybutyrate kit (Stanbio, Boerne, Texas), respectively. All assays were performed following the manufacturer's instruction.
Deis J.A., Guo H., Wu Y., Liu C., Bernlohr D.A, & Chen X. (2019). Adipose Lipocalin 2 overexpression protects against age-related decline in thermogenic function of adipose tissue and metabolic deterioration. Molecular Metabolism, 24, 18-29.
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8

Fasting Blood Glucose and Insulin Resistance

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After overnight fasting, blood samples were obtained by tail bleeding and blood glucose was measured weekly with a glucometer and glucose test strips (Roche). At the end of the experiment, plasma was obtained from the left common carotid artery and fasting blood glucose was measured with an automatic biochemical analyzer (Cobas 400 plus, Roche, Switzerland). Fasting insulin levels were determined using a mouse insulin ELISA kit (Thermo) according to the manufacturer’s instruction. Absorbance was measured at 450 nm and insulin levels were calculated by plotting a four-parameter logistic curve fit after generation of a standard curve. Insulin resistance was evaluated at the end of experiment by a homeostatic model assessment for insulin resistance (HOMA-IR): HOMA-IR = fasting glucose level (mmol/L) × fasting insulin level (mIU/L) / 22.5.
Tian X., Yan C., Liu M., Zhang Q., Liu D., Liu Y., Li S, & Han Y. (2017). CREG1 heterozygous mice are susceptible to high fat diet-induced obesity and insulin resistance. PLoS ONE, 12(5), e0176873.
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9

Detecting Mouse Insulin and Cytokines

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Mouse insulin in the plasma from NOD/scid mice of the T1D model was detected by a Mouse INSULIN ELISA Kit (EMINS; Thermo Fisher Scientific). Mouse IL-12, IL-10, IFN-γ, IL-2, IL-6, and Th17A/F in the culture supernatants were measured by commercial ELISA kits (R&D Systems) according to the manufacturer's protocol.
Shigemoto-Kuroda T., Oh J.Y., Kim D.K., Jeong H.J., Park S.Y., Lee H.J., Park J.W., Kim T.W., An S.Y., Prockop D.J, & Lee R.H. (2017). MSC-derived Extracellular Vesicles Attenuate Immune Responses in Two Autoimmune Murine Models: Type 1 Diabetes and Uveoretinitis. Stem Cell Reports, 8(5), 1214-1225.
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10

Serum Metabolic Biomarkers in Mice

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Serum adiponectin, TNFα and insulin levels in mice were analyzed by a mouse adiponectin ELISA kit (ThermoFisher Scientific), a mouse TNFα ELISA kit (Sigma-Aldrich) and a mouse insulin ELISA kit (ThermoFisher Scientific), respectively, according to manufacturer’s protocols. Serum levels of free fatty acids, triglycerides and glucose were measured by standard assay kits from Biovision. Serum cholesterol level was determined by a cholesterol quantitation kit (Sigma-Aldrich).
Than A., Xu S., Li R., Leow M.K., Sun L, & Chen P. (2017). Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis. Signal Transduction and Targeted Therapy, 2, 17022-.
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