Decitabine hy a0004

Mouse bone marrow cells derived from wild-type or Trp53(−/−) mice were transduced with MLL-AF9, and were transplanted intravenously into sublethally irradiated (525 cGy) recipient mice. MLL-AF9-expressing leukemia cells were harvested from spleens of moribund mice, and were serially transplanted into recipient mice. The serial transplantation was subsequently repeated several times to generate MLL-AF9 cells with strong leukemogenicity. The MLL-AF9 cells were then transduced with Cas9 and sgRNAs, or transduced with vector or p53DD, and were injected intravenously into non-irradiated recipient mice (5 × 10⁵/body). For experiments in Fig. 4, wild-type and Trp53(−/−) MLL-AF9 cells (1–5 × 10⁵ cells/body) were injected intravenously into non-irradiated recipient mice. For competitive transplantation, we transduced mCherry into wild-type MLL-AF9 cells as a marker, and mixed wild-type and Trp53(−/−) MLL-AF9 cells before transplantation. For treatment with decitabine, mice were subcutaneously administered with vehicle control or decitabine (0.6 mg/kg/day) every other day from day 1. Decitabine (HY-A0004) was purchased from MedChemexpress.

Bulk black phosphorous were purchased from xfnano materials tech co., ltd. 1-Methyl-2-pyrrolidinone (NMP, M103246) was purchased from Aladdin. Decitabine (HY-A0004) were purchased from MedChemExpress (MCE). EasySepTM Mouse MDSC (CD11b⁺Gr-1⁺) isolation kit (19,867), EasySepTM Mouse CD45 Positive Selection kit (18,945) and EasyEightsTM EasySepTM Magnet (18,103) were purchased from STEMCELL Technologies. Ultrasonic cell disruptor was purchased from Xiaomei ultrasonic instrument (Kunshan) Co., Ltd. Live/Dead staining kit (Calcein acetoxymethyl ester and propidium iodide probes), 2′,7′-dichlorofluorescin diacetate (DCFH-DA) probes and DAPI (Beyotime, C1006) were purchased from solarbio. HE staining kit was purchased from AiBIxin Biotechnology Co., Ltd. (abs9217, China). Cell cycle staining kit (CCS012) were purchased from MultiScience Biotech Co., Ltd. 808 nm laser and 660 nm laser were purchased from BEIJING LASERWAVE OPTOELECTRONICS TECHNOLOGY.