Sodium hydrogen sulfide

Manufactured by Merck Group

Sourced in United States

Sodium hydrogen sulfide is a chemical compound with the formula NaHS. It is a white crystalline solid that is used as a reducing agent and a source of sulfide ions in various industrial and laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Sodium sulfide (63 citations) Sodium hydrogen sulfide (NaHS) (5 citations)

8 protocols using sodium hydrogen sulfide

Comparison of Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Student’s t- test (for comparison of two sets of data) and one-way analysis of variance (comparison of three or more sets of data) were used. Data were analyzed using PRISM software and represented as mean±standard deviation. Post hoc testing was based on unpaired t-test with Bonferroni correction. Significance level was set at p<0.05.
Caffeine, sodium cyanide (NaCN), carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), sodium hydrogen sulfide (NaHS), S-Nitroso-N-acetylpenicillamine (SNAP), bromo-cDP-ribose (Br-cADPR-ribose), cyclopiazonic acid (CPA) were purchased from Sigma Aldrich Co. BTP-2 (also named YM 58483) and all receptor agonists were purchased from Tocris (Ellisville, MO, USA). Fura-2 AM was purchased from TEF Labs.

Wang J., Hogan J.O, & Kim D. (2016). Voltage- and receptor-mediated activation of a non-selective cation channel in rat carotid body glomus cells. Respiratory physiology & neurobiology, 237, 13-21.

+ Open protocol

+ Expand

Mitochondrial Dynamics in Metabolic Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium hydrogen sulfide (NaHS), N-acetyl-cysteine (NAC, an inhibitor of reactive oxygen species, ROS), Mdivi-1 and palmitate were purchased from Sigma Chemical Co. (St. Lowis, MO, USA). Cyclin D1, p27, PCNA, p21 antibodies were obtained from Cell Signaling Technology (Danvers, USA). CSE, Drp1, Mfn2, MMP2, MMP9, Collagen I, and Collagen III were from ProteinTech Bioengineering Institute (Wuhan, China). Mitosox and Mitotracker green were purchased from Roche (Mannheim, Germany). All others chemicals were from Sigma or Santa Cruz.

Sun A., Wang Y., Liu J., Yu X., Sun Y., Yang F., Dong S., Wu J., Zhao Y., Xu C., Lu F, & Zhang W. (2016). Exogenous H2S modulates mitochondrial fusion–fission to inhibit vascular smooth muscle cell proliferation in a hyperglycemic state. Cell & Bioscience, 6, 36.

+ Open protocol

+ Expand

Signaling Pathways Modulation in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium hydrogen sulfide (NaHS), H₂O₂, PD98059 (an inhibitor of ERK1/2), LY294002 (an inhibitor of PI3K) and AG1478 (an inhibitor of EGFR) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The primary antibodies for anti-cleaved caspase-3 and -9, Bcl-2, cytochrome c (Cyt-c), c-myc, c-jun, c-fos and GAPDH were from Proteintech (Wuhan, China). The anti-ERK1/2, p-ERK1/2, PI3K, p-PI3K, GSK-3β, p-GSK-3β, Akt, p-Akt, HB-EGF, EGFR and p-EGFR antibodies were obtained from Cell Signaling Technology (Denver, CO, USA). Assay kits for lactate dehydrogenase (LDH) and creatine kinase (CK) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The Cell Counting Kit-8 (CCK-8) was obtained from Boster Bio-engineering Limited Company (Wuhan, China). The HB-EGF ELISA kit was purchased from Elabscience Biotechnology (Wuhan, China). All other chemicals were from Sigma or Santa Cruz.

Zhang Y., Gao J., Sun W., Wen X., Xi Y., Wang Y., Wei C., Xu C, & Li H. (2019). H2S restores the cardioprotective effects of ischemic post-conditioning by upregulating HB-EGF/EGFR signaling. Aging (Albany NY), 11(6), 1745-1758.

+ Open protocol

+ Expand

Oxidative Stress Biomarkers in Neurodegenerative Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

6-OHDA hydrobromide, tubastatin A, and sodium hydrogen sulfide were obtained from Sigma Aldrich. RNA isolation kits, cDNA synthesis, and SYBR Green/ROX master mix were from Qiagen. Primer sequences for PCR were obtained from Eurofins MWG (Operon). Protein carbonyl, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) assays kits were obtained from Cayman Chemicals, USA. The HDAC activity assay kit was obtained from Biomol, PA, USA. The HAT activity assay kit was acquired from Active Motif, Carlsbad, CA, USA. The Fluoro-Jade C assay kit was obtained from Millipore Sigma, Burlington, MA, USA. GFAP monoclonal antibody and the corresponding anti-mouse secondary antibody were obtained from Santa Cruz Biotechnology, USA. ELISA kits for IL-1β (ER1094), IL-2 (ER0039), IL-17 (ER0035), IL-8 (ER1623), IL-6 (ER0042), TH (ER0534), GFAP (ER0229), α-synuclein (ER0921), and TNF-α (ER1393) were obtained from Fine Biotech, China. All other chemical reagents used were analytical grade.

Sun Y., Li D., Su Y., Zhao H., Pang W., Zhao W, & Wu S. (2020). Protective effect of hydrogen sulfide is mediated by negative regulation of epigenetic histone acetylation in Parkinson’s disease. Archives of Medical Science : AMS, 19(4), 1124-1135.

+ Open protocol

+ Expand

Biochemicals for Enzymatic Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

DL-propargylglycine, aminooxyacetic acid, β-cyano-L-alanine, L-cysteine, and sodium hydrogen sulfide were purchased from Sigma-Aldrich Co.

Kim D., Kim I., Wang J., White C, & Carroll J.L. (2015). Hydrogen sulfide and hypoxia-induced changes in TASK (K2P3/9) activity and intracellular Ca2+ concentration in rat carotid body glomus cells. Respiratory physiology & neurobiology, 215, 30-38.

+ Open protocol

+ Expand

Endoplasmic Reticulum Stress Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium hydrogen sulfide (NaHS), 4-PBA (an inhibitor of ERS), GSK2656157 (an inhibitor of PERK), STF083010 (an inhibitor of IRE 1α) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The primary antibodies for anti-Cyclin D1, p21^Cip/WAF−1, cleaved caspase-3 and -9, Bcl-2, cytochrome c (Cyt c), GRP 78, CHOP, cleaved caspase-12, ATF 4, ATF 6, XBP-1 and GAPDH were from Proteintech (Wuhan, China). Hoechst 33342 was from Beyotime (Wuhan, China). The anti-PERK, eIF 2α and IRE 1α antibodies were obtained from Cell Signaling Technology (Denver, USA). H₂O₂ and PBS solution were from Sigma Chemical Co. (St. Louis, MO, USA). Western blotting relative solutions were from Santa Cruz Biotechnology (Dallas, USA).

Sun W., Yang J., Zhang Y., Xi Y., Wen X., Yuan D., Wang Y., Wei C., Wang R., Wu L., Li H, & Xu C. (2017). Exogenous H2S restores ischemic post-conditioning-induced cardioprotection through inhibiting endoplasmic reticulum stress in the aged cardiomyocytes. Cell & Bioscience, 7, 67.

+ Open protocol

+ Expand

Sourcing Chemicals for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemicals used in this study were purchased from Hi media®, India except Sodium hydrogen sulfide, bought from Sigma-Aldrich®.

Vaitheeswari S., Sriram R., Brindha P, & Kurian G.A. (2015). Studying inhibition of calcium oxalate stone formation: an in vitro approach for screening hydrogen sulfide and its metabolites. International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 41(3), 503-510.

+ Open protocol

+ Expand

Inhibition of Oxidative Stress Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium hydrogen sulfide (NaHS), N-acetyl-cysteine (NAC, an inhibitor of reactive oxygen species, ROS), Ammonium pyrrolidinedithiocarbamate (PDTC, a NF-κB inhibitor), AG 490 (a JNK2 inhibitor) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The anti-cleaved caspase-3 and -9, Bcl-2, cytochrome c (Cyt c), and GAPDH were from proteintech (Wuhan, China). Hoechst 33342 were from Santa Cruz (Bergheimer, Germany). The anti-IκBα-NF-κB and JNK2-STAT3 antibodies were obtained from Cell Signaling Technology (Danvers, USA). Assay kits for malondialdehyde (MDA), superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), catalase (CAT), lactate dehydrogenase (LDH), creatine kinase (CK) and ROS were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals were from Sigma or Santa Cruz.

Li L., Li M., Li Y., Sun W., Wang Y., Bai S., Li H., Wu B., Yang G., Wang R., Wu L., Li H, & Xu C. (2016). Exogenous H2S contributes to recovery of ischemic post-conditioning-induced cardioprotection by decrease of ROS level via down-regulation of NF-κB and JAK2-STAT3 pathways in the aging cardiomyocytes. Cell & Bioscience, 6, 26.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!