The primary antibodies used were: a) rabbit polyclonal – NMIIA (Sigma), phospho-NMIIA (Ser1943) (Cell Signaling Technology), giantin, Man-II, and Rab6a (Abcam); b) mouse monoclonal - Rab6a (Santa Cruz Biotechnology), β-actin (Sigma), Sar1a, giantin (Abcam); c) mouse polyclonal - ST3Gal1 (Abnova). The secondary antibodies (Jackson ImmunoResearch) were: a) HRP-conjugated donkey anti-rabbit and donkey anti-mouse for Western-blotting; b) donkey anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 for immunofluorescence. Blebbistatin (Sigma) and latrunculin A (Tocris Bioscience) were dissolved in dimethyl sulfoxide (DMSO) immediately before use. Cells treated with a corresponding concentration of DMSO served as controls. The regular working concentrations were: Blebbistatin −25 μM for up to 24 h; latrunculin A −0.2 μM for up to 24 h. Active human Rab6a full length protein was obtained from Abcam.
Sar1a
Manufactured by Abcam
Sar1a is a protein that plays a crucial role in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus. It is involved in the formation and budding of COPII-coated vesicles, which are responsible for the transport of cargo proteins from the endoplasmic reticulum to the Golgi.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Merck Group (2 mentions)
Giantin, by Abcam (2 mentions)
Blebbistatin, by Merck Group (1 mentions)
Latrunculin a, by Bio-Techne (1 mentions)
Nmiia, by Merck Group (1 mentions)
Blebbistatin, by Abcam (1 mentions)
Latrunculin a, by Abcam (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Pyrazole, by Merck Group (1 mentions)
Tgn46, by Abcam (1 mentions)
Pdia3, by Abcam (1 mentions)
2 protocols using sar1a
1
Immunofluorescence and Western Blot Analysis
2
Organelle Trafficking Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The primary antibodies were: a) rabbit polyclonal – giantin, PDIA3, ASGP-R1 (Abcam); b) rabbit monoclonal – GM130, SAR1B (Abcam); c) mouse monoclonal – SAR1A, giantin and TGN46 (Abcam), β-actin (Sigma), GRASP65, Sec24d (Santa Cruz Biotechnology), giantin (Abcam); goat polyclonal – COPII (Sec23a, Abcam). The secondary antibodies (Jackson ImmunoResearch) were: a) HRP-conjugated donkey anti-rabbit, donkey anti-mouse, and donkey anti-goat for Western-blotting; b) donkey anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594. Pyrazole (Sigma), MG-132 (EMD Chemicals) and Brefeldin A (Sigma) were dissolved in dimethyl sulfoxide (DMSO) immediately before use. Cells treated with a corresponding concentration of DMSO served as controls. The regular working concentrations were: Pyrazole – 5 mM for 72 h, MG-132 – 2.5 μM for 24 h and Brefeldin A – 36 μM for 1 h.
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