Male spf wistar rats

Male SPF Wistar rats (8-10 weeks old, weighing 180-200 g) were purchased from the Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained in an air-conditioned room with 12 h light/12 h dark cycles. Food and water were unlimited. All experimental procedures were in accordance with the Institute for Laboratory Animal Research guidelines (Guide for the Care and Use of Laboratory Animals).
Lipopolysaccharide (V. cholerae, classical Biotype, serotype Ogawa) was provided by the Lanzhou Biological Product Research. Rabbit CSF-1 antibody was purchased from LifeSpan BioSciences, Inc., USA. Rabbit LRR-1 antibody was purchased from Wuhan Proteintech Group, Inc. Rabbit NF-κB p65 antibody was purchased from Abcam Co., Ltd., UK. HRP-marked goat anti-rabbit IgG antibody and CY3-marked goat anti-rabbit fluorescence IgG antibody were purchased from Wuhan Servicebio technology, Co., Ltd. RevertAid First Strand cDNA Synthesis Kit was purchased from ThermoFisher Scientific Co., Ltd., USA. FastStart Universal SYBR Green Master(Rox) was purchased from F. Hoffmann-La Roche Ltd., Switzerland. Rat INF-γ ELISA kit, Rat IL-6 ELISA kit, and Rat IL-17 ELISA kit were purchased from Cusabio.

Female, specific pathogen-free (SPF) BALB/c mice, weighing 18 g to 20 g each, were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd, Beijing, China. Male SPF Wistar rats, weighing about 200 g each, were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd, Beijing, China. Cynomolgus monkeys, half-male and half-female, each weighing 3 kg to 5 kg, were purchased from Nanning Fuzee Wild Animal Breeding Co. Ltd, Nanjing, China. All animals were raised under humanitarian conditions. All animal experiments used in this study were performed according to the protocols approved by the Institutional Animal Care and Use Committee of Beijing Institution of Biotechnology (Identification code for mice: IACUC of AMMS-08-2014-005; Date of approval: 18 November 2014. Identification code for rats: IACUC of AMMS-08-2014-006; Date of approval: 20 November 2014. Identification code for cynomolgus monkeys: ACU14-419; Date of approval: 26 June 2014).

Male SPF Wistar rats (N = 168, weight 140 ± 10 g) were provided by Beijing Vital River Laboratory Animal Technology Co., Ltd., production license No. SCXK (Beijing) 2012-0001. The research protocol was approved by the Animal Ethics Committee of Guang’anmen Hospital of China Academy of Chinese Medicine (No. 2015EC035-02). In accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (Bethesda, MD, USA), they were raised indoors by the Animal Feeding Center of Guang’anmen Hospital, China Academy of Chinese Medical Sciences, at a constant temperature and with good ventilation, and they were fed with standard feed, with free access to food and water.