Ultracruz dapi containing mounting medium

Manufactured by Santa Cruz Biotechnology

UltraCruz DAPI containing mounting medium is a laboratory product designed for use in fluorescence microscopy applications. It serves as a mounting medium that helps preserve and protect fluorescently labeled samples while incorporating the DNA-binding dye DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride).

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Lab products found in correlation

Glass bottom culture dishes, by MatTek (1 mentions) Fluoview 1200 confocal microscope, by Olympus (1 mentions)

Spelling variants of this product

UltraCruz mounting medium (66 citations) UltraCruz (14 citations) Mounting medium (9 citations) DAPI-containing mounting medium (3 citations) UltraCruz™ Mounting Medium containing 4′,6-diamidino-2-phenylindole (DAPI) (3 citations)

2 protocols using ultracruz dapi containing mounting medium

Visualizing PRMT5 Localization in KLF4-Expressing Cells

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Cells grown on glass bottom culture dishes (MatTek) were transfected with pEGFP-KLF4. Twenty-four hours after transfection, cells were fixed with 4% paraformaldehyde in PBS for 10 min and rendered permeable by further treatment with 0.2% Triton X-100 for 5 min. The PRMT5 antibody (Millipore, 07–405, 1:200) was diluted in PBSB (1% BSA in PBS) and incubated with cells for 1 h. After three washes with PBSB, cells were incubated with Alexa 594-labelled anti-rabbit IgG (Jackson Lab, 111-585-144, 1:500) diluted in PBSB for 1 h. Cells were washed, mounted with UltraCruz DAPI containing mounting medium (Santa Cruz), viewed, and photographed under a FluoView 1200 confocal microscope (Olympus).

Hu D., Gur M., Zhou Z., Gamper A., Hung M.C., Fujita N., Lan L., Bahar I, & Wan Y. (2015). Interplay between arginine methylation and ubiquitylation regulates KLF4-mediated genome stability and carcinogenesis. Nature Communications, 6, 8419.

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Immunofluorescence Staining of Cellular Proteins

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Cells grown on coverslip were fixed with 4% paraformaldehyde in PBS for 10 min. The membrane staining of the indicated proteins was performed without membrane permeabilization by 0.1% Triton X-100 otherwise stated. The indicated antibodies were incubated with cells 4°C overnight. After three washes with PBSB, cells were incubated with Alexa 488 anti-rabbit /594-anti-mouse IgG diluted in PBSB for 1 h. Cells were washed, mounted with UltraCruz DAPI containing mounting medium (Santa Cruz), viewed, and photographed under Nikon A1 (Nikon) with the software of NIS-Elements. The fluorescence of Dox alone was also checked.

Song X., Zhou Z., Li H., Xue Y., Lu X., Bahar I., Kepp O., Hung M.C., Kroemer G, & Wan Y. (2020). Pharmacological suppression of B7-H4 glycosylation restores antitumor immunity in immune-cold breast cancers. Cancer discovery, 10(12), 1872-1893.

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