Bombyx ovary-derived cultured germ cells (BmN4 cells) were cultured at 26 °C in EX-CELL 420 Serum-Free Medium for Insect cells (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) (Equitech-Bio) and Penicillin-Streptomycin-Glutamine (Gibco).
Ex cell 420 serum free medium for insect cells
Manufactured by Merck Group
Sourced in United States
EX-CELL 420 Serum-Free Medium is a liquid cell culture medium specifically designed for the growth and maintenance of insect cells. It is a serum-free formulation, providing the necessary nutrients and growth factors required for insect cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Equitech-Bio (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
5 protocols using ex cell 420 serum free medium for insect cells
1
Culturing Bombyx ovary-derived germ cells
2
Cultivation of BmN4 Insect Cells
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BmN4 cells were cultured at 26 °C in EX-CELL® 420 Serum-Free Medium for insect cells (Sigma) supplemented with 10% fetal bovine serum (Equitech-Bio) and penicillin-streptomycin-glutamine (Thermo Fisher Scientific).
3
Culturing BCIRL-TcA-CLG1 Beetle Cells
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We used BCIRL-TcA-CLG1 (TcA) cell line derived from T. castaneum adult and pupae. Cells were reared in T25 flasks (25 cm2 tissue culture flasks version with the VENT screw cap, TPP Cat # 90025) with 3–5 mL of Ex-CELL 420 Serum-Free Medium for Insect Cells (Sigma Aldrich, Cat # 14420C, Burlington, Massachusetts) containing L-glutamine supplemented with 5% fetal bovine serum (FBS) and kept at 28 °C. fetal bovine serum (FBS) was obtained from Atlas biologicals (Fort Collins, CO, USA). The cells were passaged on a weekly basis with half of the medium being replaced by fresh medium every 7–10 days [3 (link),26 (link)]. The contamination of the cells by TcIV was confirmed by reverse transcription quantitative PCR (RT-qPCR).
4
Culturing BmN4 Insect Cells
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BmN4 cells were cultured at 26 °C in EX-CELL 420 serum-free medium for insect cells (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Gibco) and 1x penicillin-streptomycin-glutamine (Thermo Fisher Scientific).
5
Cultivation of Viral Cell Lines
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The Spodoptera frugiperda (Sf9) cell line (Cat. No. B82501; Thermo Fisher Scientific, Carlsbad, CA, USA) was cultivated as a suspension culture at 27°C in Ex-cell 420 serum-free medium for insect cells (Sigma Aldrich Co., St Louis, Missouri, USA). The chicken hepatocellular carcinoma (LMH) cell line (Cat. No. 601411; CLS cell lines service GmbH, Eppelheim, Germany) was cultivated as an adherent culture at 37°C with 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) F12 (HyClone, Logan, UT, USA) supplemented with 5% heat-inactivated fetal bovine serum (FBS).
The Peruvian ILTV strain VFAR-043 was isolated from an outbreak occurred on a farm in Chincha Alta, Peru [14 ] and cultivated in LMH cells following a previously reported method [13 (link)]. The Newcastle disease virus (LaSota strain) and the avian metapneumovirus (SHS-FAR strain) were cultivated following published procedures [15 (link)], and the infectious bronchitis virus (Q1-type strain) was propagated onto embryonated SPF chicken eggs according to a previous study [16 ].
The Peruvian ILTV strain VFAR-043 was isolated from an outbreak occurred on a farm in Chincha Alta, Peru [14 ] and cultivated in LMH cells following a previously reported method [13 (link)]. The Newcastle disease virus (LaSota strain) and the avian metapneumovirus (SHS-FAR strain) were cultivated following published procedures [15 (link)], and the infectious bronchitis virus (Q1-type strain) was propagated onto embryonated SPF chicken eggs according to a previous study [16 ].
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