Sequenom snp assay design software version 3

Peripheral blood of the three experimental groups including BD and VKH disease patients and the controls was subjected to genomic DNA extraction with the QIAmp DNA Blood Mini Kit (Qiagen Inc., Valencia, CA, USA) and the DNA was stored at −80°C. The Applied Biosystems 7500 Real-Time PCR system was utilized to genotype CCL17/rs223828 (TagMan assay ID: C_30530263_10) by the TaqMan SNP Genotyping Assay (Applied Biosystems, Foster City, CA, USA). Genotype identification of the other 25 SNPs was conducted with the iPLEX Gold genotyping assay and Sequenom MassARRAY (Sequenom, CA, USA). Sequenom SNP Assay Design software version 3.0 was used to design primers of iPLEX reactions. Primer sequences used were shown in Table 1. The protocol and experimental requirements were performed strictly based on the instructions.

Peripheral blood of AMD patients and the controls were subjected to genomic DNA extraction by using the QIAmp DNA Blood Mini Kit (Qiagen Inc., Valencia, CA, USA) and the DNAs were stored at − 80∘C. Genotype identifications of the 39 SNPs were conducted with the iPLEX Gold genotyping assay and Sequenom MassARRAY (Sequenom, CA, USA). Sequenom SNP Assay Design software (version 3.0) was used to design the primers of iPLEX reactions [27 (link)]. Primer sequences used were shown in Additional file 1: Table S1.