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3 4 5 dimethylthiahiazo z y1 3 5 di phenytetrazoliumromide mtt

Manufactured by Solarbio
Sourced in United States, China

3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) is a colorimetric assay used to measure cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to a purple formazan product by the action of dehydrogenase enzymes in living cells.

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2 protocols using 3 4 5 dimethylthiahiazo z y1 3 5 di phenytetrazoliumromide mtt

1

Synthesis and Characterization of Functionalized Polymeric Nanoparticles

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L-Lactide (LLA) was purchased from Daigang Biotechnology Co., Ltd. (Jinan, China) and was recrystallized from dry ethyl acetate before use. Polyethylene glycol (PEG, Mw = 6000) and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Lycorine hydrochloride (LYC), Folic acid (FA), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI), and stannous octoate [Sn(Oct)2] were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The 4-dimethylamino pyridine (DMAP) was purchased from Bide Pharmatech Ltd. (Shanghai, China). Pyridine was purchased from Tianli Chemical Reagent Co., Ltd. (Tianjin, China). N-acetyl cysteine (NAC) was obtained from Sigma, MO, USA.
The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) was obtained from Solarbio, Beijing, China. Dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe was obtained from the Beyotime Institute of Biotechnology, Haimen, China. Annexin V-FITC/PI (Propidium Iodide) apoptosis detection kit was purchased from Yeasen Biotechnology, Shanghai, China. Antibodies against β-actin and IkBα were purchased from ABclonal Technology Inc., Wuhan, China.
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2

Transdermal Drug Delivery Formulation

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Minoxidil (MIN), piroxicam (PIR), baicalin (BAI), QUE, TA, OMT, and squalene were bought from Macklin (Shanghai, China). Adapalene (ADA) was purchased from Meiruier Chemical Technology Co., Ltd (Shanghai, China). LA and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) were purchased from Yuanye Biotechnology Co., Ltd (Shanghai, China). Ferulic acid (FA), castor oil, tea tree oil, and isopropyl myristate (IPM) were purchased from Aladdin (Shanghai, China). Acetonitrile and methanol were obtained from Sigma Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Gibico (NY, USA). 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) was sourced from Solarbio (Beijing, China). Other reagents were obtained from commercial sources and used without further purification.
Sprague-Dawley (SD) rats and BALB/c mice were purchased from the animal center of Sun Yat-sen University (Guangzhou, China).
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