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Gsk2334470

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GSK2334470 is a synthetic small molecule compound. It is a selective and potent inhibitor of the serine/threonine protein kinase SGK1 (serum and glucocorticoid-regulated kinase 1). SGK1 is involved in the regulation of various cellular processes including ion transport, cell survival, and transcriptional control.

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Lab products found in correlation

Mk 2206, by Selleck Chemicals (2 mentions) Ezview red anti flag m2 affinity gel, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Eif4e, by Cell Signaling Technology (1 mentions) P eif4e, by Cell Signaling Technology (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Anti β actin, by ABclonal (1 mentions) P pkcζ λ thr410 403, by Cell Signaling Technology (1 mentions) Anti pdk1, by Santa Cruz Biotechnology (1 mentions) Alpelisib byl 719, by MedChemExpress (1 mentions) Eft 508, by Selleck Chemicals (1 mentions) P gsk3β s9, by Cell Signaling Technology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) α tubulin antibody, by Santa Cruz Biotechnology (1 mentions) Jnj 38877605, by Selleck Chemicals (1 mentions)

4 protocols using gsk2334470

1

Hepatoprotective Effects of G. pentaphyllum

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G. pentaphyllum leaves (Cat: 20230615) were acquired from Beijing Bencao Fangyuan Co., Ltd. (Beijing, China). CCl4 was obtained from Boer’s (Shanghai, China). Olive oil was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Ginsenoside Rb3 (Cat: S9208) and Gypenoside XLIX (Cat: S9177) were purchased from Selleck (Shanghai, China). Gypenoside A (Cat: HY-N2440), Gypenoside XLVI (Cat: HY-N6252) and GSK2334470 (Cat: HY-14981) were purchased from MedChem Express (Shanghai, China). The aspartate transaminase (AST, Cat: BC1565), alanine transami-nase (ALT, Cat: BC1555) and hydroxyproline (HYP, Cat: BC0255) were purchased from Solarbio Life Sciences (Beijing, China).
Hu L., Zhao X., He X., Guo Y., Cheng H., Chen S., Zhou G., Wang J, & Lu Y. (2024). Gynostemma Pentaphyllum ameliorates CCl4-induced liver injury via PDK1/Bcl-2 pathway with comprehensive analysis of network pharmacology and transcriptomics. Chinese Medicine, 19, 70.
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2

Investigating AKT Signaling Pathway

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The antibodies against p-AKT T308 (Catalog 13038), p-AKT S473 (Catalog 4060), AKT (Catalog 9272), PDK1 (Catalog 3062S), eIF4E (catalog 2067), p-eIF4E (Catalog 9741), p-PKCζ/λ (Thr410/403) (Catalog 9378), GSK3β (Catalog 9315), p-GSK3β S9 (Catalog 9336), MCL-1 (Catalog 5453), and Cyclin D1 (Catalog 2978) were from Cell Signaling Technology. Other antibodies used include anti-MAPK4 (Abgent, Catalog AP7298b), anti-MAPK4 (Origene, Catalog TA505872), anti-PDK1 (Santa Cruz, Catalog sc-17765), anti-HA (Santa Cruz, Catalog sc-805), anti-FLAG (Millipore Sigma, catalog F3165), and anti-β-ACTIN (Abclonal, Catalog AC026). The kinase inhibitors used include PI3K inhibitor Alpelisib/BYL-719 (MedChemExpress, Catalog HY-15244), PDK1 inhibitor GSK2334470 (MedChemExpress, Catalog HY-14981), AKT inhibitor MK2206 (Selleckchem, Catalog S1078), and MNK1/2 inhibitors SLV-2436/SEL201 (Medchemexpres, Catalog HY-112113) and EFT-508 (Selleckchem, Catalog S8275). EZview Red anti-FLAG M2 Affinity Gel (catalog F2426) and MG132 (catalog M7449) were purchased from Millipore Sigma.
Han D., Wang W., Jeon J.H., Shen T., Huang X., Yi P., Dong B, & Yang F. (2023). Cooperative activation of PDK1 and AKT by MAPK4 enhances cancer growth and resistance to therapy. PLOS Biology, 21(8), e3002227.
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3

Kinase Inhibitor Evaluation Protocol

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Lapatinib, OSI-906, jnj38877605, MK2206, and U0126 were purchased from Selleck Chemicals. LY294002 and triciribine were purchased from Enzo Life Sciences. GSK2334470 was purchased from MedChem Express. The following antibodies were purchased from Cell Signaling Technology: phospho-EGFR (#3777), phospho-InsR/IGF1R (#3024), phospho-Met (#3077), phospho-Akt (#4060), phospho-Erk1/2 (#9101), phospho-CRAF (#9427), phospho-IRS1 (#3070) and IRS1 (#2382). phospho-IRS1 (pY632) antibody (ab109543) and vinculin antibody (ab73412) were purchased from Abcam. GAPDH antibody was purchased from Shanghai Kangchen. α-tubulin antibody was purchased from Santa Cruz. And β-actin antibody was purchased from Abmart.
Song Q., Sun X., Guo H, & Yu Q. (2016). Concomitant inhibition of receptor tyrosine kinases and downstream AKT synergistically inhibited growth of KRAS/BRAF mutant colorectal cancer cells. Oncotarget, 8(3), 5003-5015.
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4

Antibody and Inhibitor Protocols for Signaling Pathway Analysis

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Total antibodies against EphA2 (#6997) and phospho-specific antibodies against EphA2 (Ser-897; #6347), RSK (Ser-380; cross-reacting with RSK2 Ser-386; #11989), RSK (Thr-573; #9346), p38 (Thr-180/Tyr-182; #4511), ERK (Thr-202/Tyr-204; #9101), and MK2 (Thr-334; #3041 for Figs. 3 and 5, C–E or #3007 for Figs 5B and 6) were purchased from Cell Signaling Technology; antibodies against total ERK (C-9), p38α (A-12), RSK1 (C-21), RSK2 (C-19), MK2 (A-11), HSP27 (F-4), α-Tubulin (B-7), and β-Actin (C-4) were from Santa Cruz Biotechnology; an antibody against FLAG (F1804) was from Merck KGaA; a phospho-antibody against HSP27 (MAB23141) was from R&D Systems. Recombinant human EGF was obtained from R&D Systems; recombinant human active GST-MK2 protein was from Carna Biosciences; a phos-tag ligand, anisomycin, NaCl, and CDDP were from Wako Pure Chemical Industries; SB203580 and TMZ were from Merck KGaA; MK2 inhibitor III and GSK2334470 were from MedChemExpress; BI-D1870 was from BioVision; BIRB796, Skepinone-L, and BX-702 were from Selleck Chemicals. All chemical inhibitors were dissolved in dimethyl sulfoxide (DMSO).
Zhou Y., Oki R., Tanaka A., Song L., Takashima A., Hamada N., Yokoyama S., Yano S, & Sakurai H. (2023). Cellular stress induces non-canonical activation of the receptor tyrosine kinase EphA2 through the p38-MK2-RSK signaling pathway. The Journal of Biological Chemistry, 299(5), 104699.
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