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Facs diva 6

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FACS-DIVA 6.1.1 is a software package designed for the analysis and management of flow cytometry data. It provides a user-friendly interface for the acquisition, analysis, and reporting of flow cytometry experiments.

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Lab products found in correlation

Lymphocyte separation media, by ICN Biomedicals (2 mentions) Live dead fixable violet dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Facscanto 2, by BD (2 mentions)

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FACS Diva

2 protocols using facs diva 6

1

PBMC Isolation and Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMC) were prepared by density-gradient centrifugation (Lymphocyte Separation Media; ICN Biomedicals, Irvine, CA). Lymph node derived single cell suspensions were obtained from core biopsies. Cell suspensions were stained as previously described (BD Biosciences, Franklin Lakes, NJ).30 (link) For Ki67 staining, cells were fixed in 4% paraformaldehyde and permeabilized in 70% EtOH. To assess cell death fresh whole blood samples were collected, red cells were removed by ACK lysis, and CLL cell viability was assessed with the LIVE/DEAD® Fixable Violet Dead Cell Stain Kit (Invitrogen, Grand Island, NY). Cells were analyzed on a FACS Canto II flow cytometer (BD Biosciences) using FACS-DIVA 6.1.1 and FlowJo software (Version 8.8.6; TreeStar, Ashland, OR).
Herman S.E., Niemann C.U., Farooqui M., Jones J., Mustafa R.Z., Lipsky A., Saba N., Martyr S., Soto S., Valdez J., Gyamfi J.A., Maric I., Calvo K.R., Pedersen L.B., Geisler C.H., Liu D., Marti G.E., Aue G, & Wiestner A. (2014). Ibrutinib-induced lymphocytosis in patients with chronic lymphocytic leukemia: correlative analyses from a phase II study. Leukemia, 28(11), 2188-2196.
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2

PBMC Isolation and Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMC) were prepared by density-gradient centrifugation (Lymphocyte Separation Media; ICN Biomedicals, Irvine, CA). Lymph node derived single cell suspensions were obtained from core biopsies. Cell suspensions were stained as previously described (BD Biosciences, Franklin Lakes, NJ).30 (link) For Ki67 staining, cells were fixed in 4% paraformaldehyde and permeabilized in 70% EtOH. To assess cell death fresh whole blood samples were collected, red cells were removed by ACK lysis, and CLL cell viability was assessed with the LIVE/DEAD® Fixable Violet Dead Cell Stain Kit (Invitrogen, Grand Island, NY). Cells were analyzed on a FACS Canto II flow cytometer (BD Biosciences) using FACS-DIVA 6.1.1 and FlowJo software (Version 8.8.6; TreeStar, Ashland, OR).
Herman S.E., Niemann C.U., Farooqui M., Jones J., Mustafa R.Z., Lipsky A., Saba N., Martyr S., Soto S., Valdez J., Gyamfi J.A., Maric I., Calvo K.R., Pedersen L.B., Geisler C.H., Liu D., Marti G.E., Aue G, & Wiestner A. (2014). Ibrutinib-induced lymphocytosis in patients with chronic lymphocytic leukemia: correlative analyses from a phase II study. Leukemia, 28(11), 2188-2196.
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