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Griess ilosvay s nitrite reagent

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Griess–Ilosvay's nitrite reagent is a chemical reagent used for the detection and determination of nitrite ions in various samples. It is composed of two solutions, which when combined, form a characteristic pink-reddish color in the presence of nitrite ions. The reagent is commonly used in analytical chemistry and various scientific applications that require the identification and quantification of nitrite compounds.

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Lab products found in correlation

Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Interleukin 6 (il 6), by BD (1 mentions) Cellrox deep red flow cytometry assay kit, by Thermo Fisher Scientific (1 mentions)

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Griess reagent (831 citations) Griess's reagent (5 citations)

3 protocols using griess ilosvay s nitrite reagent

1

Plasma Cytokine and Nitrite Quantification

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Detection and concentrations of TNF (R&D, Minneapolis, MN, USA) and IL-6 (BD Biosciences) in plasma was determined by ELISA following the manufacturer’s protocol. The concentration of nitrite, a stable oxidation product of NO, was determined with the Griess–Ilosvay’s nitrite reagent (Merck, Darmstadt, Germany) as previously described [41 (link)].
Brigo N., Pfeifhofer-Obermair C., Tymoszuk P., Demetz E., Engl S., Barros-Pinkelnig M., Dichtl S., Fischer C., Valente De Souza L., Petzer V., von Raffay L., Hilbe R., Berger S., Seifert M., Schleicher U., Bogdan C, & Weiss G. (2021). Cytokine-Mediated Regulation of ARG1 in Macrophages and Its Impact on the Control of Salmonella enterica Serovar Typhimurium Infection. Cells, 10(7), 1823.
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2

Cytokine and Oxidative Stress Analysis

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Determination of cytokines in culture supernatants was performed with ELISA kits for TNF-α and Lcn2 (from R&D; Wiesbaden, Germany) and IL-6, IL-10 (from BD), following manufacturer’s instructions. Determination of nitrite, the stable oxidation product of NO, was carried out with the Griess-Ilosvay’s nitrite reagent (Merck) as described by Nairz et al. (2009) (link). Detection of ROS was carried out after a standard infection experiment using CellROX®DeepRed Flow Cytometry Assay Kit (Life Technologies) according to the manufacturer’s instructions. In brief, CellROX®DeepRed was added at the concentration of 500 nM to the infected or induced cells and incubated for 30 min at 37°C. SYTOX Blue Dead Cell stain (1 mM) was then added and the cells analyzed by FACS sorting within the next 120 min. The following controls were included: Cells treated with N-acetyl-L-cysteine (NAC) or tert-butyl hydroperoxide (TBHP), and an unstained cell sample.
Mitterstiller A.M., Haschka D., Dichtl S., Nairz M., Demetz E., Talasz H., Soares M.P., Einwallner E., Esterbauer H., Fang F.C., Geley S, & Weiss G. (2016). Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection. Cellular microbiology, 18(10), 1374-1389.
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3

Cytokine and Nitric Oxide Quantification

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Determination of cytokines in culture supernatants was performed with ELISA kits for TNF-α, IL-6 and IL-10 (BD Pharmingen). Determination of nitrite, the stable oxidation product of nitric oxide (NO), was carried out with the Griess-Ilosvay's nitrite reagent (Merck) as described [52 (link)].
Nairz M., Schroll A., Haschka D., Dichtl S., Sonnweber T., Theurl I., Theurl M., Lindner E., Demetz E., Aβhoff M., Bellmann-Weiler R., Müller R., Gerner R.R., Moschen A.R., Baumgartner N., Moser P.L., Talasz H., Tilg H., Fang F.C, & Weiss G. (2015). Lipocalin-2 ensures host defense against Salmonella Typhimurium by controlling macrophage iron homeostasis and immune response. European journal of immunology, 45(11), 3073-3086.
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