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N sim super resolution microscope system

Manufactured by National Instruments

The N-SIM super-resolution microscope system is a high-performance imaging solution designed for advanced biological research. It utilizes structured illumination microscopy (SIM) technology to achieve super-resolution imaging, allowing for the visualization of cellular structures and dynamics with enhanced detail and clarity beyond the diffraction limit of conventional light microscopy.

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3 protocols using n sim super resolution microscope system

1

High-Resolution Microscopy Imaging Protocols

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Color histological images were captured on a Leica DM5500B microscope with a DFC450 digital color camera (Leica Microsystems). Immunolabeled chromosome spreads and testis TUNEL assay images were captured with an ORCA Flash4.0 digital monochrome camera (Hamamatsu Photonics) on a Leica DM5500B microscope (Leica Microsystems) and processed using Photoshop (Adobe) software packages. Super-resolution imaging microscopy analysis was performed using a Nikon NSIM super-resolution microscope system and NIS-Elements 2 image processing software.
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2

Imaging and Analysis of Meiotic Chromosome Spreads

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Immunolabeled chromosome spreads and DAPI-stained diakinesis/metaphase I nuclei were imaged using a Zeiss AxioPlan II microscope with 63× Plan Apochromat 1.4 objective and EXFO X-Cite metal halide light source. Images were captured by a Hamamatsu ORCA-ER CCD camera and processed using Volocity (Perkin Elmer) and Photoshop (Adobe) software packages. SIM analysis was performed using a Nikon N-SIM super-resolution microscope system and NIS-Elements 2 image processing software. MSH4-RNF212 colocalization was determined using NIS-Elements and co-foci were confirmed by visual inspection. Testes sections were imaged using an Axiovert 200 microscope and AxioCamMRc camera using AxioVision 4.4 software. Apoptotic cells were imaged and counted in representative fields of view.
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3

Imaging and Analysis of Meiotic Chromosome Spreads

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunolabeled chromosome spreads and DAPI-stained diakinesis/metaphase I nuclei were imaged using a Zeiss AxioPlan II microscope with 63× Plan Apochromat 1.4 objective and EXFO X-Cite metal halide light source. Images were captured by a Hamamatsu ORCA-ER CCD camera and processed using Volocity (Perkin Elmer) and Photoshop (Adobe) software packages. SIM analysis was performed using a Nikon N-SIM super-resolution microscope system and NIS-Elements 2 image processing software. MSH4-RNF212 colocalization was determined using NIS-Elements and co-foci were confirmed by visual inspection. Testes sections were imaged using an Axiovert 200 microscope and AxioCamMRc camera using AxioVision 4.4 software. Apoptotic cells were imaged and counted in representative fields of view.
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