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Heparin sodium solution

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Heparin sodium solution is a laboratory reagent used as an anticoagulant. It is a sterile, nonpyrogenic solution of heparin sodium, a naturally occurring substance that is extracted from porcine intestinal mucosa. The solution is intended for in vitro use in medical laboratories and research settings.

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Lab products found in correlation

Hyclone fetal bovine serum, by GE Healthcare (1 mentions) S 2366, by Werfen (1 mentions) Egm 2, by Lonza (1 mentions) α thrombin, by Prolytix (1 mentions) Protein c, by Prolytix (1 mentions) Milli q system, by Merck Group (1 mentions) Ribonuclease a solution, by Merck Group (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Agarose solution, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by GoldBio (1 mentions)

Spelling variants of this product

Heparin (2033 citations) Heparin sodium (115 citations) Heparin solution (11 citations)

4 protocols using heparin sodium solution

1

Baboon Endothelial Cell Thrombomodulation Assay

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Vacuum-pressed SIS was generously provided by Cook Biotech. Thrombomodulin, protein C, α-thrombin, and APC were from Haematologic Technologies. Heparin sodium solution was from Sigma Aldrich. Baboon platelet poor plasma from at least 5 animals was pooled using previously collected aliquots that had been stored at −80°C. The prothrombin time (PT) reagent Innovin® was purchased from Dade®, and the activated partial thromboplastin time (APTT) reagent HemosIL® was from Instrumentation Laboratories. The chromogenic substrate S-2366 used for APC quantification was from Chromogenix. Baboon carotid endothelial cells (ECs) were isolated as described previously [24 ][25 (link)]. Baboon ECs were cultured in endothelial growth medium-2 (EGM-2) from Lonza, supplemented with the MV BulletKit and 10% HyClone™ fetal bovine serum (FBS, GE Healthcare Life Sciences). Phosphate buffered saline (PBS) with Ca2+ and Mg2+ was from Mediatech, Inc.
Glynn J.J, & Hinds M.T. (2016). Bioactive Anti-thrombotic Modification of Decellularized Matrix for Vascular Applications. Advanced healthcare materials, 5(12), 1439-1446.
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2

siRNA Protection Against RNAse Degradation

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To analyze the siRNA protection against RNAse degradation, TS-MSN (at an initial siRNA concentration of 2.5 µM) were incubated with an aqueous ribonuclease A solution (1.2 µg/mL Sigma–Aldrich Chemie GmbH, Schelldorf, Germany) at a ratio of 2:1 for 30 min, 2, 4, and 6 h at 37 °C. Afterward, ribonuclease A was inactivated by heating the suspensions at 70 °C for 30 min. An equivalent amount of free siRNA was incubated for 30 min and used as a positive control to check the ribonuclease A activity to analyze the amount of free siRNA. Samples were diluted in water (Milli-Q system, Millipore, Paris, France) or aqueous heparin sodium solution (10 mg/mL) Sigma–Aldrich Chemie GmbH, Schnelldorf, Germany) and mixed with the loading buffer (agarose gel loading dye 6X) to deposit 20 mol ofsiRNA per well on 1% agarose gel containing ethidium bromide. With its strong negative charge, heparin is used as a control to displace complexed siRNA from TS-MSN. A 150 V voltage was applied for about 15 min in a Tris/acetate/EDTA buffer (TAE 1X, 40 mM acetate, EDTA 1 mM, pH 7.6). Gels were visualized and analyzed with EvolutionCapt software on a FusionSolo.65.WL imager (Vilbert Lourmat, Marne-la-Vallée, France).
Eljack S., Allard-Vannier E., Misericordia Y., Hervé-Aubert K., Aubrey N., Chourpa I., Faggad A, & David S. (2022). Combination of Nanovectorized siRNA Directed against Survivin with Doxorubicin for Efficient Anti-Cancer Activity in HER2+ Breast Cancer Cells. Pharmaceutics, 14(11), 2537.
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3

Acute Copper Exposure: Blood and Liver Analysis

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The fish were anesthetized with MS-222 at a concentration of 60 mg/L before sampling. Two fish for each glass aquarium were sacrificed at 48 h after acute Cu exposure. Blood was collected from the caudal vein of fish using syringes previously infiltrated with heparin sodium solution (0.2%, Sigma, USA). Then, plasma was separated after a centrifugation at 3000 g for 15 min. Parts of livers were removed and fixed in 4% formaldehyde for liver histochemical and histological observations. The rest parts of liver were immediately dissected on ice and frozen in liquid nitrogen, storing in the refrigerator at -80°C until subsequent analysis.
Li H., Gong W., Wang G., Yu E., Tian J., Xia Y., Li Z., Zhang K, & Xie J. (2022). Role of nuclear pregnane X receptor in Cu-induced lipid metabolism and xenobiotic responses in largemouth bass (Micropterus salmoides). Frontiers in Endocrinology, 13, 950985.
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4

Subcutaneous Islet Transplantation in Diabetic Rats

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Diabetes was rendered in recipient rats by a single intravenous injection of streptozotocin (STZ, 60 mg/kg, Sigma-Aldrich). Diabetes was confirmed by measuring blood glucose levels >400 mg/dl in two consecutive measurements. A prevascularized SC graft bed was prepared by implanting an agarose-basic fibroblast growth factor (bFGF) disc, as described [22 , 54 (link)]. Briefly, a lyophilized agarose disc made from 4.5% agarose solution (Sigma-Aldrich) with bFGF solution [500 μg/ml bFGF (Gold Biotechnology, St. Louis, MO) in saline] and heparin sodium solution [250 μg/ml (Sigma-Aldrich)] was implanted in the dorsal SC site of recipient rats. A week after implantation, a prevascularized capsule had formed around the disc, and the disc was removed through a small opening made on the capsule. Freshly isolated islets were used for SC transplantation. Islets loaded either on an O2 transporter (n=4) or Neg-CTL device (n=4) were transplanted into the space inside of the prevascularized capsule. The capsule opening and skin wound were separately sutured to close, except for the cannula portion of the device penetrating through the opening. These surgical procedures were performed under general anesthesia.
Komatsu H., Cook C.A., Gonzalez N., Medrano L., Salgado M., Sui F., Li J., Kandeel F., Mullen Y, & Tai Y.C. (2018). Oxygen transporter for the hypoxic transplantation site. Biofabrication, 11(1), 015011.
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