A Leica M165FC dissecting microscope equipped with a DFC425 camera and Leica Application Suite software v3.8.0 was used to take bright-field and fluorescent images of the empty ECM scaffolds and Matristem® (Acell, Columbia, USA) and seeded ECM scaffolds on explanted mouse heart, tissue, respectively. Videos of cell-seeded, beating ECM scaffolds were taken with an Olympus IX51microscope equipped with fluorescence and DP72 CCD camera and CellSens software v1.6.
Dfc425 camera
Manufactured by Leica
Sourced in Germany, United States
The DFC425 is a digital camera designed for microscopy applications. It features a CMOS sensor with a resolution of 5 megapixels and supports live image capture and image acquisition. The camera is capable of capturing high-quality images and is suitable for use in a variety of laboratory settings.
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Lab products found in correlation
M165fc dissecting microscope, by Leica (1 mentions)
250d camera, by Canon (1 mentions)
Wild m10 microscope, by Leica (1 mentions)
Application suite las microscope software, by Leica (1 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)
Pm 10ad, by Olympus (1 mentions)
M205 light microscope, by Leica (1 mentions)
Szx10 microscope, by Olympus (1 mentions)
Dp80 digital camera, by Olympus (1 mentions)
Szx7 microscope, by Canon (1 mentions)
Bx53 fluorescence microscope, by Olympus (1 mentions)
Powershot a640 camera, by Canon (1 mentions)
Historesin, by Leica (1 mentions)
Las software, by Leica (1 mentions)
Dmirb inverted microscope, by Leica (1 mentions)
Leitz laborlux s, by Leica (1 mentions)
M205c microscope, by Leica (1 mentions)
Tricaine, by Merck Group (1 mentions)
Mz10f stereomicroscope, by Leica (1 mentions)
Methylcellulose, by Merck Group (1 mentions)
16 protocols using dfc425 camera
1
Imaging cell-seeded ECM scaffolds
2
In-situ Seed Fossil Analysis
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The material was studied using a Leica Wild M10 microscope equipped with a Leica DFC 425 camera. Macroscopic images were taken with Canon 250D camera. One specimen exhibits remaining permineralized tissues with cellular structures, especially of in-situ seeds, that were replaced by iron oxide. The complete fossil (MB. Pb. 1997/1562) was directly placed uncoated inside the chamber of a Zeiss EVO 50 Scanning Electron Microscope (SEM) (Carl Zeiss, Germany), and characteristics of the epidermis were observed under a low vacuum. SEM photographs were edited with Photoshop software.
3
Murine ESC Differentiation into EBs
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All murine ESC lines were differentiated as previously described62 (link). To initiate embryoid body (EB) formation, “hanging drops” composed of 2000 cells in 30 μL of differentiation medium were generated (day-0 of differentiation). The differentiation medium was based on Iscove’s modified Dulbecco’s medium (IMDM, Gibco) and supplemented with 20% heat inactivated FBS, 0.5 mM monothioglycerol, lacking supplemental LIF. On day-2 of differentiation, the EBs were transferred into gelatin coated 24-well plates with 1–2 EBs per well and cultivated for 2 additional days. From day 5 until day 12, differentiation medium was replaced every 2–3 days. Images of EBs were captured at×5 magnification with a Leica DMIRB inverted phase contrast fluorescence microscope with a DFC425 camera (Leica) and processed using the Leica Application Suite (LAS) microscope software. The microscopic images of the fluorescent EBs were further analyzed for the quantitative analysis of total fluorescence in each EB. Total fluorescence per EB (TF/EB) was calculated in an excel sheet by applying the measurements obtained from the EBs using Image J software. Total fluorescence per EB (TF/EB) = Integrated Density - (Area of selected EB × Mean fluorescence of background readings).
4
Callose and Lignin Staining in Plant Roots
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For callose determination, root tips (0.5 cm) were dipped into 85% (v/v) ethanol and incubated for over 12 h at room temperature (25 °C) with gentle shaking. Then roots were placed into 1% (w/v) aniline blue solution in 1 M glycine (pH 9.5) for 5 h at RT and thoroughly washed with double distilled water154 (link). The cross-sections of root segments, which were stained with aniline blue, were observed under a fluorescence microscope (NIKON ELIPSE Ni equipped with LEICA DFC 425 camera) with UV illumination.
Five mm root pieces were taken from the middle of the root zone. Root pieces were immersed in saline phosphate buffer (pH 7.4) containing formaldehyde (3.7%; w/w)155 (link). To detect lignin in the root, root cross sections were stained for several minutes with the aid of the Maule reaction156 , which gives different colors depending on the composition of lignin monomers157 (link). Lignified tissues were stained orange/brown staining under white light. The experiment was replicated four times for each treatment.
Five mm root pieces were taken from the middle of the root zone. Root pieces were immersed in saline phosphate buffer (pH 7.4) containing formaldehyde (3.7%; w/w)155 (link). To detect lignin in the root, root cross sections were stained for several minutes with the aid of the Maule reaction156 , which gives different colors depending on the composition of lignin monomers157 (link). Lignified tissues were stained orange/brown staining under white light. The experiment was replicated four times for each treatment.
5
Examining Insect Genitalia Morphology
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Figures of the specimens were made using a Leica M205 light microscope with a Leica DFC425 camera. Images were produced using the Leica Application Suite V3.7 and edited using Adobe Photoshop CS6.0 (Adobe Systems). Abdomens were removed from examined specimens and macerated in cold 10% NaOH solution overnight, subsequently rinsed for 30 s with pure water, and stored in glycerin. An Olympus SZX10 microscope was used for dissecting specimens and an Olympus PM-10AD was used for drawing the dissected male genitalia.
Morphological terminology in this work follows Zhang (1990) , but wing venation follows Dworakowska (1993) .
Type specimens of the new species are deposited in the collections of the Entomological Museum, Northwest A&F University, Yangling, China (NWAFU
Morphological terminology in this work follows Zhang (1990) , but wing venation follows Dworakowska (1993) .
Type specimens of the new species are deposited in the collections of the Entomological Museum, Northwest A&F University, Yangling, China (
6
Microscopic Imaging of Seed Phenotypes
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Images of seed phenotypes were captured using an Olympus SZX7 microscope equipped with a Canon Powershot A640 camera. For cleared whole-mount observation, immature seeds, that are 8 days after pollination, were cleared using chloral hydrate, glycerol, and water (8 g: 1 ml: 2 ml) and photographed using a Leica DM6000 B differential interference contrast microscope equipped with a Leica DFC 425 camera. Fluorescence was detected with an Olympus BX53 fluorescence microscope equipped with an Olympus DP80 digital camera.
7
Anatomical Analysis of Adventitious Roots
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Samples were taken from 0.5 cm above the tips of adventitious roots. The samples were fixed in FAA 50 (formaldehyde, acetic acid, 50% ethyl alcohol) [63 ] for 48 h and then stored in 70% alcohol. Afterward, they were dehydrated in ethanol series and embedded in methacrylate resin (Leica HistoResin, Leica, Wetzlar, Germany) [64 ]. The samples were sectioned on a semi-automatic rotary microtome and cross-sections (4-μm-thick) were stained with 0.05% Toluidine Blue pH 4.7 [65 (link)]. The slides were analyzed on a Leica DMR photomicroscope with DFC 425 camera (Leica, Wetzlar, Germany) attached. Quantitative analyses were performed using LAS software (V3.8 Leica, Wetzlar, Germany).
8
Measuring Cardiomyocyte Contractility Dynamics
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On day-12, beating clusters of cells were video recorded using a Leica DMIRB inverted microscope and Leica DFC425 camera with Micro-Manager 1.4 software at an acquisition rate of 50 frames per second (fps) for 10 seconds. After acquisition, videos were converted from TIFF stack to AVI using Image J. The AVI movies were analyzed by a cross-correlation algorithm to track the movement of pixels from frame to frame and to produce effective contractility metrics of the cardiomyocytes63 64 (link). Isoproterenol hydrochloride (ISO), a standard stimulator of the β-adrenergic signaling cascade, and carbachol, a synthetic acetylcholine analogue acting as a cholinergic agonist, were dissolved in serum-free medium and stored according to the manufacturer’s guidelines.
9
Nissl Staining of Substantia Nigra
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For the Nissl-staining brain, slices were fixed in 4% formaldehyde in phosphate buffer at pH 7.4. Samples were then washed twice with phosphate buffer, and submerged in 0.5% cresyl violet acid solution for 30 min. Afterwards, samples were washed in distilled water and dehydrated in graded ethanol solutions (70%, 95% and 100%). The slices were finally cleared in xylene (twice, 5 min each). Then, the slices were coverslipped with dibutylphthalate polystyrene xylene (DPX) organic mounting medium (Sigma-Aldrich, Spain). Once dried, histological images at the level of substantia nigra were obtained with a digital camera (DFC425 camera, Leica) coupled to a light microscope (Leitz Laborlux S, Leica).
10
Comparative Study on Erctella and Cornu Mollusks
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The specimens for this study were collected by the authors of the study, particularly by the senior author. Missing sequences for Erctella Cornu
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