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Masshunter b08

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The Masshunter B08 is a data acquisition and analysis software solution for mass spectrometry applications. It provides tools for instrument control, data acquisition, and data processing.

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Lab products found in correlation

Isotopic standard mixture, by Cambridge Isotopes (2 mentions) 1200 series liquidchromatography system, by Waters Corporation (2 mentions) Hypercarb trap column, by Waters Corporation (2 mentions) 6460 triple quadrupole mass spectrometer, by Agilent Technologies (2 mentions) Beh c18 analytical column, by Waters Corporation (2 mentions) Triple quadrupole 7000c, by Agilent Technologies (1 mentions) Msd chemstation, by Agilent Technologies (1 mentions) 6500 series lc q tof system, by Agilent Technologies (1 mentions) 630b accurate mass qtof ms, by Agilent Technologies (1 mentions) Kinetex c18 column, by Phenomenex (1 mentions) Agilent 1290 infinity 2 hplc system, by Agilent Technologies (1 mentions) Agilent 6460 qqq, by Agilent Technologies (1 mentions)

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MassHunter Qualitative Analysis B08 software (6 citations)

7 protocols using masshunter b08

1

Quantifying Amino Acids by LC-MS/MS

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Amino acids were analyzed as underivatized compounds by liquid
chromatography tandem mass spectrometry (LC-MS/MS). Samples (50μL) were
deproteinized with 50μL of 6% sulfosalicylic acid, and centrifuged for 15
min at 17,000 RCF. Supernatants were diluted with an isotopic standard mixture
(Cambridge Isotope Laboratories, Inc) in 2 μM tridecafluoroheptanoic acid
(TDFHA), and then injected into the LC-MS/MS system. Samples were analyzed using
a clinically validated amino acid analysis method48 (link). An Agilent 1200 series liquid
chromatography system equipped with a Thermo Hypercarb trap column (3 μm,
4.6 × 50 mm) and a Waters BEH C18 analytical column (2.5 μm, 2.1
× 100 mm) was used to separate compounds in a trap and reverse-elute
configuration. An Agilent 6460 triple quadrupole mass spectrometer was used in
positive polarity electrospray ionization for detection by dynamic multiple
reaction monitoring (MRM). Mobile phase A was 0.02% perfluoroheptanoic acid and
mobile phase B was acetonitrile. Standard curves were prepared by diluting amino
acid standards (Wako Chemicals USA, Inc); for 1-methylhistidine,
S-aminoethylcystine was used as the internal standard and for 3-methylhistidine,
histidine-13C615N3 was used as
the internal standard. Agilent Masshunter B08 was used for data analysis.
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
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2

Quantifying Amino Acids by LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Amino acids were analyzed as underivatized compounds by liquid
chromatography tandem mass spectrometry (LC-MS/MS). Samples (50μL) were
deproteinized with 50μL of 6% sulfosalicylic acid, and centrifuged for 15
min at 17,000 RCF. Supernatants were diluted with an isotopic standard mixture
(Cambridge Isotope Laboratories, Inc) in 2 μM tridecafluoroheptanoic acid
(TDFHA), and then injected into the LC-MS/MS system. Samples were analyzed using
a clinically validated amino acid analysis method48 (link). An Agilent 1200 series liquid
chromatography system equipped with a Thermo Hypercarb trap column (3 μm,
4.6 × 50 mm) and a Waters BEH C18 analytical column (2.5 μm, 2.1
× 100 mm) was used to separate compounds in a trap and reverse-elute
configuration. An Agilent 6460 triple quadrupole mass spectrometer was used in
positive polarity electrospray ionization for detection by dynamic multiple
reaction monitoring (MRM). Mobile phase A was 0.02% perfluoroheptanoic acid and
mobile phase B was acetonitrile. Standard curves were prepared by diluting amino
acid standards (Wako Chemicals USA, Inc); for 1-methylhistidine,
S-aminoethylcystine was used as the internal standard and for 3-methylhistidine,
histidine-13C615N3 was used as
the internal standard. Agilent Masshunter B08 was used for data analysis.
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
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3

GC-MS/MS Analysis of Haloacetic Acids

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Analytical determination of methyl esters of HAAs was performed using a 7890B GC connected in series to a 7000C triple quadrupole (Agilent Technologies). Ionization was carried out in the electron ionization mode. One µL of the derivatized extract was injected in splitless mode using a7638B automated injector (split flow=50 mL/min, splitless time=1.5 min). GC separation of the analytes was achieved using a capillary The analyzer was operated in selected reaction monitoring (SRM) mode, using nitrogen (1.5 mL/min) as the collision gas. A minimum of two SRM transitions was acquired per analyte (see Table 4). Figure 4 shows the total ion chromatogram obtained after the analysis of a standard calibration solution at a concentration of 10 µg/mL. Mass acquisition was performed using MSD ChemStation and data analysis was done with Mass Hunter B.08 (Agilent Technologies).
Postigo C., Andersson A., Harir M., Bastviken D., Gonsior M., Schmitt-Kopplin P., Gago-Ferrero P., Ahrens L., Ahrens L, & Wiberg K. (2021). Unraveling the chemodiversity of halogenated disinfection by-products formed during drinking water treatment using target and non-target screening tools. Journal of hazardous materials, 401.
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4

Chemical Profiling of Curcuma longa and Limestone

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The chemical constituent of C. longa and red limestone extracts was analyzed by liquid chromatography/time-of-flight mass spectrometer (Agilent 6500 Series LC Q-TOF System). Chromatographic separation was accomplished with a Zorbax eclip plus C-18 column (2.1 mm × 50 mm, 1.7 μm, Agilent Technologies, USA). A gradient elution of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) was performed at a flow rate of 200 μl/min. Total run time was 26 min. The gradient program was started at 5% B for a minute and then it was linearly increased to 17% B within 10 min. After 3 min, it was increased to 100% B within 20 min and the eluent composition was maintained for 2 min before it was decreased to 5% B over 2 min. The filtered sample solution through a 0.22 μm PTFE membrane was analyzed in a volume of 1 μl. The gas temperature was 350 °C and gas flow was of 13 l/min. Full scan mass spectra were acquired over the mass-to-charge ratio (m/z) from 100 to 1000 amu in positive and negative ion mode. The nebulizer was 45 psig. The data analysis was performed by using Agilent Mass Hunter B.08.00 software (qualitative navigator, qualitative workflows) and PCDL database. Peak identification was evaluated by comparing the retention time, fragmentation patterns and mass spectra with references compounds from mass spectra library.
Duangyod T., Rujanapan N., Champakam S, & Charoensup R. (2021). Anti-inflammatory activity and chemical constituents of red limestone. Journal of Advanced Pharmaceutical Technology & Research, 12(2), 185-189.
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5

HPLC-ESI-QTOF-MS Analysis of Compounds

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Analysis was performed using HPLC/ESI-QTOF-MS system in positive ion mode with a 630B accurate mass QTOF-MS (Agilent Technologies INc., Santa Clara, CA, USA) mass spectrometer and an ESI-Jet-Stream ion source. The separation was done using Gemini 100 × 2.1, 3.5 µm column, thermostated at + 40 °C. The mobile phase consisted of a mixture of H2O + 0.1% HCOOH (A) and ACN + 0.1% HCOOH (B). The gradient elution was carried out at a constant flow of 0.4 mL/min. The gradient applied was as follows 0 min., 5%B; 15 min., 98%B; 20 min., 98%B. The return to the initial gradient compositions (95% A/5% B) was held for 5 min. The total time of analysis was 20 min. ESI-QTOF-MS analysis was performed with the following parameters, gas (N2) temperature: 300 °C, flow rate 10 mL/min, 35 psig, sheath gas temperature: 325 °C, flow rate 10 L/min, fragmentor voltage 140 V, Vcap 4000 V. Two collision energies, 20 and 40 eV were chosen. Data acquisition was performed in Auto MS/MS mode at the range of 100–1000 mass units. Mass Hunter B.08.00 software (Agilent Technologies INc., Santa Clara, CA, USA) was used for data analysis. Mass calibration was done before analysis according to the manufacturer’s recommendations to ensure mass accuracies below 5 ppm. Data handling was performed by MassHunter Workstation Software B.08.00 and OriginPro 9.5.
Kurach Ł., Chłopaś-Konowałek A., Budzyńska B., Zawadzki M., Szpot P, & Boguszewska-Czubara A. (2021). Etazene induces developmental toxicity in vivo Danio rerio and in silico studies of new synthetic opioid derivative. Scientific Reports, 11, 24269.
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6

Crosslinked Protein Identification by LC-MS/MS

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The crosslinked sites in the crosslinked protein samples by BCETG were identified by liquid chromatography linked to the tandem mass spectrometry (LC-MS/MS) through analyzing the crosslinked peptides. After being boiled for 10 min to stop crosslinking, the protein samples that were crosslinked for 1 h were then denatured, reduced and digested with trypsin based on the previous method [8 (link)]. LC-MS/MS analysis was performed by a 1260 series HPLC coupled to an ESI-Q/TOF mass spectrometry (Agilent Technologies, Palo Alto, CA, USA) using the previously reported conditions [8 (link)]. The crosslinked peptides were identified and quantified using the software PLink 2 (The Institute of Computing Technology of the Chinese Academy of Sciences, Beijing, China) and the Masshunter B.08.00 of Agilent (Palo Alto, CA, USA), respectively, by processing the mgf data. The conditions used for the identification by the software PLink were as the following. Protein database: BSA for BSA protein, soybean proteins for SPI sample, and whey proteins for WP sample; protease: trypsin; variable modifications: methionine oxidation; fixed modification: cysteine carbamidomethylation; missed cleavages: no more than two; fragment ion tolerance: 50 ppm; precursor mass tolerance: 50 ppm.
Wang H., Zhang Y., Yuan Z., Zou X., Ji Y., Hou J., Zhang J., Lu F, & Liu Y. (2022). Crosslinking Mechanism on a Novel Bacillus cereus Transglutaminase-Mediated Conjugation of Food Proteins. Foods, 11(22), 3722.
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7

Quantification of Fusarium Mycotoxins in Rice

Cited 1 time
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Rice cultures [24 (link)] were inoculated with single-spore isolates (SNA, agar plugs of 0.5 cm diameter) of F. temperatum, F. subglutinans, F. verticillioides, and F. proliferatum obtained from naturally infected maize cobs, and references strain MUCL52463 (Table S4), kindly provided by Dr. Jonathan Scauflaire (Earth and Life Institute, Louvain-la-Neuve, Belgium). Controls were inoculated with blank culture medium. Tubes were incubated in the dark for 28 days, at 21 °C. Mycotoxins were extracted in 30 mL acetonitrile/water/acetic acid (84/15/1 (v/v/v)), following evaporation and sample preparation in methanol/water (20/80 (v/v)) for HPLC-MS/MS, as described elsewhere [77 (link)].
Toxin quantification was performed on an Agilent 1290 Infinity II HPLC system coupled to an Agilent 6460 QQQ (Agilent Technologies, Waldbronn, Germany). Samples were analyzed on a Phenomenex Kinetex C18 column with a particle size of 2.5 µm, 100 Å pore size and 50 × 2.1 mm (Phenomenex Ltd., Aschaffenburg, Germany). A 12-point calibration ranging from 3.9 to 2000 µg/L was used. Final analysis was performed with MassHunter B.0.8.00 (Agilent, Waldbronn, Germany). The MS/MS transitions, limits of detection (LODs) and limits of quantification (LOQs) are listed in Table S4.
Pfordt A., Schiwek S., Rathgeb A., Rodemann C., Bollmann N., Buchholz M., Karlovsky P, & von Tiedemann A. (2020). Occurrence, Pathogenicity, and Mycotoxin Production of Fusarium temperatum in Relation to Other Fusarium Species on Maize in Germany. Pathogens, 9(11), 864.
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