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Heparin

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Heparin is a laboratory equipment product that functions as an anticoagulant. It is used to prevent the formation of blood clots during laboratory procedures.

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Lab products found in correlation

α minimum essential medium, by Thermo Fisher Scientific (3 mentions) Tryple, by Thermo Fisher Scientific (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Sodium chloride (nacl), by Pfizer (2 mentions) Sodium nitroprusside, by Pfizer (2 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (2 mentions) Protamine, by Fresenius (2 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (2 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Buffered formalin, by Thermo Fisher Scientific (1 mentions) Ethylene glycol bis 2 aminoethylether n n n n tetraacetic acid, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions) Transferrin, by Merck Group (1 mentions) Automatic cell counter, by Beckman Coulter (1 mentions) Human ab serum, by Atlanta Biologicals (1 mentions) Insulin, by Eli Lilly (1 mentions) 70 m cell strainer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Heparin sodium (12 citations) 30 USP heparin/saline (4 citations) Preservative-free sodium heparin (3 citations) PBS/heparin (2 citations) Heparin‐0.9% saline solution (2 citations) Intravenous heparin (2 citations) Unfractionated heparin (2 citations)

46 protocols using heparin

1

Tumor and Immune Cell Analysis in Mice

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Female mice were examined for palpable tumors starting at 8 weeks. At 15 weeks of age, matched sibling pairs of WT and COL mice with tumors were anesthetized with 4 % isofluorane. Blood was collected via retro-orbital procedure into an ethylenediaminetetraacetic acid (EDTA) (1.3 mg/ml of blood) collection tube (Sarstedt, Numbrecht, Germany). Mice were then perfused intracardially with 0.9 % sodium chloride (Abbott Laboratories, Chicago, IL, USA) supplemented with 1 unit of heparin (Hospira, Lake Forest, IL, USA). Mammary gland tumors and spleens were harvested and placed in high glucose DMEM (Gibco, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS, Gemini Bio Products, Baltimore, MD, USA) and 1× antibiotic antimycotic solution (Corning, Corning, NY, USA) for ELISA cytokine array and flow cytometry experiments. Tumors from the fourth or fifth (right or left) mammary glands and the lungs were harvested and fixed in 10 % buffered formalin (Thermo Fisher Scientific, Kalamazoo, MI, USA). Spleens were weighed and cut in half: one half was placed in DMEM with 10 % FBS and 1× antibiotic antimycotic for flow cytometry experiments, and the other half was fixed in 10 % buffered formalin. Tumors were weighed and approximately 150 mg of tumor was used per mice per flow cytometry experiment.
García-Mendoza M.G., Inman D.R., Ponik S.M., Jeffery J.J., Sheerar D.S., Van Doorn R.R, & Keely P.J. (2016). Neutrophils drive accelerated tumor progression in the collagen-dense mammary tumor microenvironment. Breast Cancer Research : BCR, 18, 49.
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2

Immune Cell Calcium Signaling

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Heparin and 0.9% sodium chloride were purchased from Hospira (Lake Forest, IL). Ethylene glycol-bis (2-aminoethylether)-N,N,N,N-tetraacetic acid (EGTA), protease inhibitor cocktail, and dimethyl sulfoxide were purchased from Sigma (St Louis, MO). Fura-2-AM, anti-human formyl peptide receptor-1 (FPR-1) and anti-FPR-2 antibodies were purchased from R&D (Minneapolis, MN). Other supplies were obtained as below.
Li H., Itagaki K., Sandler N., Gallo D., Galenkamp A., Kaczmarek E., Livingston D.H., Zeng Y., Lee Y.T., Tang T.I., Isal B., Otterbein L, & Hauser C.J. (2015). MITOCHONDRIAL DAMPS FROM FRACTURES SUPPRESS PULMONARY IMMUNE RESPONSES VIA FORMYL PEPTIDE RECEPTORS 1 AND 2. The journal of trauma and acute care surgery, 78(2), 272-281.
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3

Expansion and Characterization of Human Mesenchymal Stromal Cells

Cited 2 times
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Human MSCs (NIH Center for Bone Marrow Stromal Cell Transplantation) were culture-expanded in α-minimum essential medium (Life Technologies) with 20% fetal bovine serum (Gemini, Sacramento, CA), 0.29 mg/mL L-glutamine (Life Technologies), and 1% penicillin/streptomycin (Life Technologies) at 37 °C under 5% CO2 and 1% O2. Early passage MSCs (<5) were used and were characterized for cell-surface markers by conventional flow cytometry protocols using an Accuri C6 flow cytometer (Supplemental Figure 1). [22 (link)] For i.v. infusions, MSCs were treated with TrypLE (Life Technologies) and resuspended at 107 cells/mL in Roswell Park Memorial Institute-1640 medium (Life Technologies) containing 1 U/mL Heparin (sodium salt) (Hospira). 106 MSC were i.v. injected in the lateral tail vein 3–4h post-pFUS. ~5 min before MSC injections, all groups were administered i.v. sodium nitroprusside (Hospira, Lake Forest, IL) at 1 mg/kg. [12 (link), 21 (link)]
Burks S.R., Nguyen B.A., Tebebi P.A., Kim S.J., Bresler M.N., Ziadloo A., Street J.M., Yuen P.S., Star R.A, & Frank J.A. (2015). Pulsed focused ultrasound pretreatment improves mesenchymal stem cell efficacy in preventing and rescuing established acute kidney injury in mice. Stem cells (Dayton, Ohio), 33(4), 1241-1253.
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4

Erythroid Differentiation from CD34+ Cells

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CD34+ hematopoietic stem and progenitor cells
from mobilized peripheral blood were purchased from the Fred Hutchinson
Cancer Research Center. CD34+ cells were cultured via a
previously described culture system that allows complete maturation into
enucleated red blood cells (Giani et al.,
2016; Ludwig et al.,
2014). The base media for erythroid differentiation was composed of
2% human AB plasma (obtained from the blood bank at Boston
Children’s Hospital), 3% human AB serum (Atlanta
Biologicals), 1% of penicillin/streptomycin, 3 U/mL heparin
(Hospira), 10 μg/mL insulin (Lilly), 200 μg/mL transferrin
(Sigma) in Iscove’s Modified Dulbecco’s Medium (IMDM;
Gibco). In Phase I (day 0–6), cultures were supplemented with 10
ng/mL stem cell factor (SCF), 1 ng/mL interleukin-3 (IL-3), and either with
recombinant EPO from Amgen (3 U/mL), EPO WT (10−9 M) or
EPO R150Q (10−9 or 10−7 M) to initiate
erythroid lineage commitment. In Phase II (day 7–13) and Phase III
(day 14 onwards), cultures were supplemented with SCF and EPO (either with
WT or R150Q) or EPO alone, respectively, to promote further erythroid
differentiation. Cells were incubated at 37°C with 20%
O2 and 5% CO2. Cell concentration was
measured using an automatic cell counter (Beckman Coulter).
Kim A.R., Ulirsch J.C., Wilmes S., Unal E., Moraga I., Karakukcu M., Yuan D., Kazerounian S., Abdulhay N.J., King D.S., Gupta N., Gabriel S.B., Lander E.S., Patiroglu T., Ozcan A., Ozdemir M.A., Garcia K.C., Piehler J., Gazda H.T., Klein D.E, & Sankaran V.G. (2017). Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation. Cell, 168(6), 1053-1064.e15.
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5

Peripheral Blood Leukocyte Immunophenotyping

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Peripheral blood samples were obtained by decapitating the animals and collected with heparin (1000 U/ml, Hospira, ROVI Contract manufacturing, Madrid, Spain). Fifty μl of blood were incubated with the antibodies detailed in the NPCs section for 20 min at RT and protected from the light. Then, erythrocytes were removed by using the red blood cell lysis buffer mentioned above. After 10 min, 2.5 ml of FACS buffer (PBS-0.5 % BSA) were added and samples were centrifuged at 500 x g for 5 min at RT. Cell pellets were washed with FACS buffer and centrifuged again. Cells were resuspended in 200 μl of FACS buffer and then analyzed by flow cytometry after filtration. Flow cytometry data were acquired with a FACSCanto II and data analysis was performed using Cytomics FC500 with the CXP program.
Brea R., Valdecantos P., Rada P., Alen R., García-Monzón C., Boscá L., Fuertes-Agudo M., Casado M., Martín-Sanz P, & Valverde Á.M. (2021). Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress. Aging (Albany NY), 13(6), 7800-7827.
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6

Isolation of Intrahepatic Lymphocytes

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At the indicated time points, liver, spleen, bone marrow and lymph nodes (LNs) were excised. Livers were incubated with 400 MandL/ml of collagenase and 50 µg/ml of DNase (Roche) for 30 minutes at 37 °C. Then, all the specimens were mechanically disaggregated and filtered through a 70 µm cell strainer (Thermo Fisher Scientific). Intrahepatic lymphocytes were isolated after a Percoll (GE Healthcare) gradient centrifugation of liver suspensions. All samples were then stained for flow cytometry. For studies requiring peripheral blood, 100-150 µL of peripheral blood samples were collected on 50 µL of 5% Heparin (Hospira) at the indicated time points.
Otano I., Azpilikueta A., Glez-Vaz J., Alvarez M., Medina-Echeverz J., Cortés-Domínguez I., Ortiz-de-Solorzano C., Ellmark P., Fritzell S., Hernandez-Hoyos G., Nelson M.H., Ochoa M.C., Bolaños E., Cuculescu D., Jaúregui P., Sanchez-Gregorio S., Etxeberria I., Rodriguez-Ruiz M.E., Sanmamed M.F., Teijeira Á., Berraondo P, & Melero I. (2021). CD137 (4-1BB) costimulation of CD8+ T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulation. Nature Communications, 12, 7296.
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7

C5a Binding Assay with Heparin

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HSPG (5μg/ml; Sigma-Aldrich) was coated on 96 well assay plates (Coring) overnight at 25°C. After washing 3 times with PBS with Tween 20 (PBST), 1%FCS/PBS was added to each well for 60-min at 25°C to block and then washed 3 times. C5a (1nM; R&D) with or without heparin (80μg/ml; Hospira) was loaded onto the well and incubated for 20-min at 25°C. After washing 3 times with PBST, C5a detection antibody (200ng/ml per well; R&D) was incubated for 2-hr at 25°C and washed 3 times with PBST. Streptavidin-HRP (R&D) was incubated for 20-min at 25°C and washed 3 times with PBST. Substrate solution (Invitrogen) was loaded onto plate for 20-min and 2N H2SO4 was used as a stop solution. Absorbance at OD 450nm was evaluated with a SpectraMax Plus 384 (Molecular Device).
Miyabe Y., Miyabe C., Murooka T.T., Kim E.Y., Newton G.A., Kim N.D., Haribabu B., Luscinskas F.W., Mempel T.R, & Luster A.D. (2017). Complement C5a Receptor is the Key Initiator of Neutrophil Adhesion Igniting Immune Complex-induced Arthritis. Science immunology, 2(7), eaaj2195.
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8

Tumor Cell Isolation and Analysis

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Peripheral blood was collected from tail veins at various time points into tubes containing heparin (Hospira, Inc.). Tumors were removed 4 days post-RT and processed into single cell suspensions as previously described [1 (link)]. A total of 1 x 106 tumor cells and 15uL of whole blood were blocked with Fc Block (clone 2.4G2) followed by staining with a cocktail of directly conjugated primary antibodies (Supplementary Table S2) for 30 minutes. All samples were washed with 1 mL of PBS/1% BSA/0.1% azide, fixed with BD Cytofix/Cytoperm (BD Biosciences), and analyzed using a 12-color LSRII (BD Biosciences) and FlowJo software (Tree Star). Data is reported as percent of CD45+ events and normalized per milligram of tumor where indicated.
Connolly K.A., Belt B.A., Figueroa N.M., Murthy A., Patel A., Kim M., Lord E.M., Linehan D.C, & Gerber S.A. (2016). Increasing the efficacy of radiotherapy by modulating the CCR2/CCR5 chemokine axes. Oncotarget, 7(52), 86522-86535.
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9

MSC Homing After Pulsed Focused Ultrasound

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Human MSCs from 23 year old female [18 (link)] (provided by NIH Center for Bone Marrow Stromal Cell Transplantation under an approved Intramural Research Branch protocol http://sigs.nih.gov/bmsctc) were culture-expanded in α-minimum essential medium (Life Technologies, Carlsbad, CA) with 20% lot-selected fetal bovine serum (Gemeni Bio-products, Sacramento, CA) at 37 °C under 5% CO2 and 95% air. Early passage cells (3–5) were used for studies. For i.v. infusions, MSCs were detached with TrypLE (Life Technologies, Carlsbad, CA) and resuspended at 107 cells/mL in Hank's Balanced Salt Solution (Life Technologies) containing 10 U/mL Heparin (sodium salt) (Hospira, Lake Forest, IL). MSC (1×106 in 100 μL) were injected into the lateral tail vein either 45 min before pFUS, or 3, 8, or 16 hr post-pFUS. For experiments that assessed the impact of drugs on MSC homing, 1×106 cells were injected approximately 4 hr post-pFUS. For all MSC homing experiments, sodium nitroprusside (Hospira, Lake Forest, IL) was intravenously injected in the lateral tail vein at a dose of 1 mg/kg in PBS 5 minutes before MSC injection. pFUS-treated and untreated contralateral hamstrings for all experiments were harvested 24 hr post-MSC injection and processed for histological analyses.
Tebebi P.A., Burks S.R., Kim S.J., Williams R.A., Nguyen B.A., Venkatesh P., Frenkel V, & Frank J.A. (2015). Cyclooxygenase-2 or tumor necrosis factor-α inhibitors attenuate the mechanotransductive effects of pulsed focused ultrasound to suppress mesenchymal stromal cell homing to healthy and dystrophic muscle. Stem cells (Dayton, Ohio), 33(4), 1173-1186.
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10

Assay for Intrinsic Coagulation Factor

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The following materials were used: pooled normal plasma; George King (Overland Park, KS); human FXI; Haematologic Technologies (Burlington, VT); S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide); DiaPharma; heparinase I/III, hyaluronidase, and chondroitinase ABC, Sigma-Aldrich (St Louis, MO)’ heparin (1000 USP units/mL), Hospira, Inc. (Lake Forest, IL); and protamine (10 mg/mL); Fresenius Kabi (Lake Zurich, IL). Polyphosphate (poly-P, 60 to 100 phosphate units) was a gift from Dr. Thomas Renne (U. Hamburg-Eppendorf).
Mohammed B.M., Cheng Q., Matafonov A., Verhamme I.M., Emsley J., McCrae K.R., McCarty O.J., Gruber A, & Gailani D. (2019). A non-circulating pool of factor XI associated with glycosaminoglycans in mice. Journal of thrombosis and haemostasis : JTH, 17(9), 1449-1460.
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