Log In Sign up
The largest database of trusted experimental protocols

Ab19925

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

Ab19925 is a primary antibody designed for use in immunoassay applications. It is a rabbit polyclonal antibody that recognizes a specific target protein. The antibody is provided in a liquid format.

Automatically generated - may contain errors

Lab products found in correlation

Sc 10768, by Santa Cruz Biotechnology (2 mentions) β actin, by Cell Signaling Technology (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions) E cadherin, by Proteintech (1 mentions) N cadherin, by Proteintech (1 mentions) Vimentin, by Proteintech (1 mentions) Ecl substrate, by Beyotime (1 mentions) Fibronectin, by Proteintech (1 mentions) Wnt3a, by Abcam (1 mentions) β catenin, by Proteintech (1 mentions) Sc 137179, by Santa Cruz Biotechnology (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Ecl chemiluminescence, by Merck Group (1 mentions) Bca protein assay kit, by Beyotime (1 mentions)

5 protocols using ab19925

1

Western Blot Analysis of WNT3A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin samples (120 mg) were homogenized in RIPA lysis buffer (1 ml) with protease inhibitor. The total protein was quantified using the BCA method (Pierce TM BCA protein assay kit, Thermo
Fisher) and 60 µg proteins were fractionated on 10% SDS-PAGE gels and transferred to a polyvinylidene difluoride membrane. The membrane was incubated in anti-WNT3A (ab19925,
Abcam; 1:200 dilution) at 4°C overnight after blocking with 5% milk (Morinaga & Co., Ltd.). HRP-conjugated antibody (Goat anti-Rabbit IgG (H+L) (Code No. 65-6120, ThermoFisher; 1:1,000
dilution) was used as the secondary antibody to incubate the membrane at room temperature for 1h. The membranes were analyzed using β-actin (Cell signaling, Danvers, MA, USA; 1:1,000
dilution) as the reference primary antibody.
Tang Y., Wang C., Desamero M.J., Kok M.K., Chambers J.K., Uchida K., Kominami Y., Ushio H., Cervancia C., Estacio M.A., Kyuwa S, & Kakuta S. (2022). The Philippines stingless bee propolis promotes hair growth through activation of Wnt/β-catenin signaling pathway. Experimental Animals, 72(1), 132-139.
+ Open protocol
+ Expand
2

Immunofluorescent Detection of WNT3A

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protein expression of WNT3A was accomplished through Immuno-fluorescence. FFPE tissue sections were deparaffinized, rehydrated, treated in citrate buffer, pH 6.0 for antigen retrieval,
and blocked in milk (Morinaga & Co., Ltd., Tokyo, Japan). The sections were incubated overnight at 4°C with the primary antibody, anti-Wnt3a (ab19925; polyclonal, Abcam, Boston, MA, USA;
1:300 dilution). Alexa FluorTM 594-conjugated goat anti-Rabbit IgG (H+L) (Code No. A-11012, Thermo Fisher; 1:1,000 dilution, Waltham, MA, USA) was used as secondary antibody.
Finally, sections were mounted using Vectashield® Antifade mounting medium with DAPI (Vector Laboratories, Inc., Newark, CA, USA).
Tang Y., Wang C., Desamero M.J., Kok M.K., Chambers J.K., Uchida K., Kominami Y., Ushio H., Cervancia C., Estacio M.A., Kyuwa S, & Kakuta S. (2022). The Philippines stingless bee propolis promotes hair growth through activation of Wnt/β-catenin signaling pathway. Experimental Animals, 72(1), 132-139.
+ Open protocol
+ Expand
3

Western Blot Analysis of EMT Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The Western blot was processed as previously described.12 (link) The primary antibodies used in the present study were listed below: wnt3a (1:1000, ab19925, Abcam), β-catenin (1:1000, 51067-2-AP, Proteintech), Vimentin (1:1000, 10366-1-AP, Proteintech), E-cadherin (1:500, 20874-1-AP, Proteintech), N-cadherin (1:500, 22018-1-AP, Proteintech), Fibronectin (1:500, 15613-1-AP, Proteintech), Snail (1:500, 13099-1-AP, Proteintech) and GAPDH (1:2000, 60004-1-Ig, Proteintech). The blots were developed using the ECL substrate (Beyotime, China) and photographed using the imaging system (Bio-Rad, CA, USA).
Li H., Cui X., Hu Q., Chen X, & Zhou P. (2019). CLK3 Is A Direct Target Of miR-144 And Contributes To Aggressive Progression In Hepatocellular Carcinoma. OncoTargets and therapy, 12, 9201-9213.
+ Open protocol
+ Expand
4

Immunohistochemical Staining for BRG1 and WNT3A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was carried out using a rabbit anti-BRG1 polyclonal antibody (sc-10768, Santa Cruz, USA, diluted 1:250) and a rabbit anti-WNT3A polyclonal antibody (ab19925, Abcam, Cambridge, United Kingdom, diluted 1:100). Positive staining was scored by two independent investigators who had no knowledge of the patient outcomes. When discrepancy in an assessment was encountered, the slides were re-examined by both pathologists under a multi-head microscope to obtain agreements. The staining intensity was scored as being 0 (negative), 1 (weak), 2 (moderate), or 3 (strong). The extent of staining was scored according to the percentage of positively stained cells: 0 (0–10%), 1 (11–25%), 2 (26–75%), and 3 (75–100%). The sum of the intensity and extent of the scores were defined as the final staining score. The specimens were divided into 2 groups according to their overall scores, which were as follows: 0–3 = negative and weak, 4–6 = moderate and strong positive.
Lin S., Jiang T., Ye L., Han Z., Liu Y., Liu C., Yuan C., Zhao S., Chen J., Wang J., Tang H., Lu S., Yang L., Wang X., Yan D., Peng Z, & Fan J. (2016). The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A. Oncotarget, 7(52), 86051-86063.
+ Open protocol
+ Expand
5

Western Blot Analysis of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted from cultured cells and tissues using RIPA lysis buffer (Beyotime Biotechnology, Jiangsu, China). Protein concentrations were determined using BCA protein assay kit (Beyotime Biotechnology, Jiangsu, China). Equal amounts of protein were separated by electrophoresis on SDS polyacrylamide gel and transferred onto polyvinylidene difluoride membranes. The membranes were blocked with 5% non-fat milk solution for 1 h, and then incubated with a primary antibody overnight at 4°C and a secondary antibody at room temperature, successively. The bands were detected by ECL chemiluminescence (Millipore, USA) according to the manufacturer’s instructions. Expression of GAPDH or actin was applied as internal control to confirm equal loading of whole protein.
Antibodies used in the Western blot assay were as follows: anti-BRG1 polyclonal antibody (sc-10768, Santa Cruz, USA, diluted 1:250), anti-WNT3A polyclonal antibody (ab19925, Abcam, Cambridge, United Kingdom, diluted 1:100), anti-GAPDH antibody (sc-137179, Santa Cruz; USA, diluted 1:2000), anti-actin antibody (60008-1-Ig, Proteintech; USA, diluted 1:2000).
Lin S., Jiang T., Ye L., Han Z., Liu Y., Liu C., Yuan C., Zhao S., Chen J., Wang J., Tang H., Lu S., Yang L., Wang X., Yan D., Peng Z, & Fan J. (2016). The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A. Oncotarget, 7(52), 86051-86063.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!