Anti nephrin

Manufactured by Merck Group

Anti-Nephrin is a laboratory reagent used in research applications. It is a monoclonal antibody that specifically binds to the Nephrin protein, which is a key component of the glomerular filtration barrier in the kidneys. Anti-Nephrin can be used to detect and quantify Nephrin expression in various experimental models and biological samples.

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Lab products found in correlation

Streptozocin stz, by Merck Group (1 mentions) Ss 31, by ChinaPeptides (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti caspase 3, by Merck Group (1 mentions) Anti opa1, by Santa Cruz Biotechnology (1 mentions) Anti drp1, by Merck Group (1 mentions) Anti cytochrome c, by Merck Group (1 mentions) Anti podocin, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions)

2 protocols using anti nephrin

Molecular Mechanisms of Diabetic Nephropathy

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SS31 was obtained from ChinaPeptides (Shanghai, China). Streptozocin (STZ) was purchased from Sigma (Shanghai, China). Antibodies used were as follows: anti-SYNPO (NBP2-39100, Noves), anti-Nephrin (PR52265, Sigma), anti- Caspase-3 (9664s, CST), anti-OPA1 (ab42364, Abcam), anti-OMA1 (sc-515788, Santa) and anti-Tubulin (Sigma, T6074), HRP-conjugated anti-Mouse and anti-rabbit (Sigma) secondary antibody. 1,640 (11879-020) and fetal bovine serum (FBS) (A3160902) were purchased from Gibco.

Yang Q., Xie W., Wang X., Luo J., Zhou Y., Cao H., Sun Q., Jiang L, & Yang J. (2022). SS31 Ameliorates Podocyte Injury via Inhibiting OMA1-Mediated Hydrolysis of OPA1 in Diabetic Kidney Disease. Frontiers in Pharmacology, 12, 707006.

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Mitochondrial Dynamics and Apoptosis Assay

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Anti‐nephrin, anti‐podocin, anti‐cytochrome c, anti‐Caspase3, Anti‐p‐Drp1, anti‐Drp1, and anti‐β‐actin antibodies were obtained from Sigma (Sigma–Aldrich, St. Louis, MO). Anti‐opa1 were purchased from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz, CA). MitoTracker Red ROS was purchased from Sigma (Sigma–Aldrich). PD used for the cell experiments was obtained from Solaibao Company (Beijing, China); PD used for animal treatments was purchased from Mansite Bio‐Technology Co., Ltd (purity > 98.0%, HPLC; Nanjing, China). H₂O₂ solution (30%), N‐acetyl‐L‐cysteine (NAC), Drp1 small‐interfering RNA (siRNA), and scramble control were purchased from Santa Cruz Biotechnology.

Ni Z., Tao L., Xiaohui X., Zelin Z., Jiangang L., Zhao S., Weikang H., Hongchao X., Qiujing W, & Xin L. (2017). Polydatin impairs mitochondria fitness and ameliorates podocyte injury by suppressing Drp1 expression. Journal of Cellular Physiology, 232(10), 2776-2787.

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