3α adiol

Manufactured by Steraloids

Sourced in United States

3α-adiol is a chemical compound used in laboratory settings. It serves as a precursor for the synthesis of various steroid hormones and is utilized in research and development applications.

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Lab products found in correlation

3β adiol, by Steraloids (2 mentions) Sulfatase from abalone entrails, by Merck Group (1 mentions) High performance liquid chromatography (hplc), by Thermo Fisher Scientific (1 mentions) Testosterone, by Steraloids (1 mentions) β glucuronidase from e coli, by Merck Group (1 mentions) Charcoal dextran stripped fetal bovine serum cd fbs, by Atlanta Biologicals (1 mentions) Optima lc ms, by Thermo Fisher Scientific (1 mentions) R1881, by Merck Group (1 mentions) Testosterone, by Merck Group (1 mentions)

2 protocols using 3α adiol

Androgen Effects on Tumor Cell Proliferation

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Tumour cell proliferation was evaluated by BrdU-ELISA. Cells were treated with 0.1, 1, or 10 nmol/L testosterone (article number: T6147, Sigma-Aldrich, Taufkirchen, Germany); 0.1, 1, 5, or 10 nmol/L DHT (article number: D5027, Sigma-Aldrich, Taufkirchen, Germany) and R1881 (article number: R0908, Sigma-Aldrich, Taufkirchen, Germany); and 0.01, 0.1, 1, 5, 10, 100, 250, or 1000 nmol/L 3α-adiol and 3β-adiol (article number: A1170-000 for 3α-adiol and A1220-000 for 3β-adiol, both from Steraloids, Newport, Rhode Island, USA) for 48 h.

Bremmer F., Jarry H., Unterkircher V., Kaulfuss S., Burfeind P., Radzun H.J., Ströbel P, & Thelen P. (2018). Testosterone metabolites inhibit proliferation of castration- and therapy-resistant prostate cancer. Oncotarget, 9(24), 16951-16961.

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Steroid hormone quantification protocol

Cited 1 time

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Reagents were of ACS grade or higher (e.g. HPLC and Optima LC/MS) and were purchased from Thermo Fisher Scientific and used without further purification. Testosterone, epiTestosterone (epi-T), DHEA, DHEA-S sodium salt, DHEA glucuronide (DHEA-G), AST, epi-AST, DHT, 5-adiol, 3α-adiol and 3β-adiol were purchased from Steraloids (Wilton, NH, USA). [2,3,4-¹³C₃]-T ([¹³C₃]-T) and [2,3,4-¹³C₃]-DHT ([¹³C₃]-DHT) were from C/D/N Isotopes (Point-Claire, Quebec, Canada) and Cambridge Isotopes (Andover, MA, USA), respectively. [2,3,4-¹³C₃]-3α-adiol ([¹³C₃]-3α-adiol) and [2,3,4-¹³C₃]-3β-adiol ([¹³C₃]-3β-adiol) were synthesized by an enzymatic method according to our published procedure (Zang et al. 2017 (link)). 4-Dimethylaminopyridine (DAP) 2-methyl-6-nitrobenzoic anhydride (MNBAn), picolinic acid (PA), triethylamine (TEA), anhydrous tetrahydrofuran (THF), β-glucuronidase from E. coli and sulfatase from Abalone entrails were from Sigma-Aldrich. Charcoal dextran stripped fetal bovine serum (CD-FBS) was from Atlanta Biologicals (Lawrenceville, GA, USA).

Zang T., Taplin M.E., Tamae D., Xie W., Mesaros C., Zhang Z., Bubley G., Montgomery B., Balk S.P., Mostaghel E.A., Blair I.A, & Penning T.M. (2017). Testicular vs adrenal sources of hydroxy-androgens in prostate cancer. Endocrine-related cancer, 24(8), 393-404.

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