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Anti vamp2 antibody

Manufactured by Synaptic Systems
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Sourced in Germany

The Anti-VAMP2 antibody is a laboratory reagent used for the detection and analysis of the VAMP2 protein, which is involved in the process of synaptic vesicle fusion. This antibody can be utilized in various biochemical and cell biology techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to study the expression, localization, and interactions of the VAMP2 protein.

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Lab products found in correlation

Mouse on mouse immunodetection kit, by Vector Laboratories (1 mentions) Anti cyclin d1 antibody, by Thermo Fisher Scientific (1 mentions) Az100, by Nikon (1 mentions) Hematoxylin solution, by Muto Pure Chemicals (1 mentions) Shandon cytospin 2 cytocentrifuge, by Thermo Fisher Scientific (1 mentions) Bx51 research microscope, by Olympus (1 mentions)

Spelling variants of this product

Anti-VAMP2 (11 citations) VAMP2 (11 citations)

2 protocols using anti vamp2 antibody

1

Histological Analysis of Cellular Markers

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Luxol fast blue (LFB) staining was performed according to the manufacturer’s protocol (LFB Solution; Muto Pure Chemicals, Tokyo, Japan). H&E staining was performed using hematoxylin solution (Muto Pure Chemicals) and 1% eosin in water (Wako Pure Chemicals). Immunofluorescence staining was performed using a Mouse-on-Mouse immunodetection kit (Vector Laboratories, Burlingame CA), anti-VAMP2 antibody (1:200, cl:69.1; Synaptic Systems, #104211, Goettingen, Germany), anti-cyclin D1 antibody (1:50, cl:SP4; Thermo Fisher Scientific KK, #RM-9104-SO, Yokohama, Japan), AlexaFluor488-conjugated donkey anti-rabbit IgG F(ab’)2 fragment (1:500; Jackson ImmunoResearch, West Grove, PA), and Rhodamine RedX-conjugated donkey anti-mouse IgG F(ab’)2 fragments (1:500, Jackson ImmunoResearch). Microscopic images were obtained using a Nikon AZ-100 (Tokyo, Japan) and a cooled CCD camera (SPOT RT3; Diagnostic Instruments, Sterling Heights, MI).
Tajika Y., Murakami T., Iijima K., Gotoh H., Takahashi-Ikezawa M., Ueno H., Yoshimoto Y, & Yorifuji H. (2017). A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging. Scientific Reports, 7, 3645.
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2

BoNT-induced SNAP-25 and VAMP2 cleavage

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Mast cells were treated with 0.1 pM BoNT A or B for 24 hours, then attached to a glass slide by using Shandon Cytospin 2 cytocentrifuge (Thermo Fisher). The cells were stained with 1 μg/ml anti-BoNT A-cleaved-SNAP-25 antibody and anti-VAMP2 antibody (Synaptic Systems) according to the manufacturer’s instructions. Slides were mounted in ProLong anti-fade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). We imaged the cells using the Bx51 research microscope (Olympus) and X-Cite 120 fluorescence illumination systems (EXFO Photonic Solutions). All isotype controls showed negative staining (data not shown). The fluorescence intensity of each cell was measured using Image J software (NIH) and the corrected total cell fluorescence (CTCF) was calculated.
Choi J.E., Werbel T., Wang Z., Wu C.C., Yaksh T.L, & Nardo A.D. (2018). Botulinum toxin blocks mast cells and prevents rosacea like inflammation. Journal of dermatological science, 93(1), 58-64.
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