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Phospho akt clone d9e

Manufactured by Cell Signaling Technology
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Phospho-AKT (clone D9E) is a primary antibody that specifically recognizes AKT protein phosphorylated at Serine 473. It can be used for the detection and quantification of phosphorylated AKT in various experimental applications.

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Lab products found in correlation

Discovery xt, by Roche (1 mentions) Ez prep, by Roche (1 mentions) Optiview dab detection kit, by Roche (1 mentions) Phospho erk clone d13.14.4e, by Cell Signaling Technology (1 mentions) Phospho met clone d26, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Phospho-Akt (892 citations) Phospho-Akt (Ser473) (clone D9E; (5 citations) Clone D9E (3 citations) Anti-phospho-AKT (clone D9E), (2 citations) Phospho AKT (S473) clone D9E (2 citations)

2 protocols using phospho akt clone d9e

1

Automated Phosphorylated Antibody Detection

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Immunohistochemistry was performed on an automated VENTANA Discovery XT staining instrument according to the manufacturer’s recommendations. Five different phosphorylated antibodies were utilized in this study from Cell Signaling Technologies: Phospho-AKT (clone D9E, dilution 1:50, Ser473, #4060), Phospho-ERK (clone 20G11, dilution 1:400, Thr202/Tyr204, # 4376), Phospho-SRC (clone D49G4, dilution 1:200, Tyr416. #6943), Phospho-STAT3 (clone D3A7, dilution 1:30, Tyr705, #9145), and Phospho-SMAD2 (clone 138D4, dilution 1:80, Ser465/467, #3108). Slides were deparaffinized using EZPrep (Ventana Medical Systems) at 90 °C, antigen retrieval, and antibodies conditions followed package inserts. Slides were developed using OptiView DAB detection kit (Ventana Medical Systems) and counterstained with hematoxylin. Whole slide images were obtained using an Aperio slide scanning system.
Lerch M.L., Kenerson H.L., Chafin D., Westerhoff M., Theiss A., Otter M., Yeung R.S, & Baird G.S. (2020). Effect of immediate cold formalin fixation on phosphoprotein IHC tumor biomarker signal in liver tumors using a cold transport device. Scientific Reports, 10, 2147.
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2

Western Blot Analysis of Signaling Proteins

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Western blotting was performed as previously described (25 (link)). Primary antibodies for phospho-ERK (clone D13.14.4E; catalog #4370S), total ERK (clone 137F5; catalog #4695S), phospho-AKT (clone D9E; catalog #4060S), total AKT (clone 40D4; catalog #2920S), phospho-Met (clone D26; catalog #3077S), and total Met (clone D1C2; catalog #8198S) from Cell Signaling Technology.
Phadke M.S., Chen Z., Li J., Mohamed E., Davies M.A., Smalley I., Duckett D.R., Palve V., Czerniecki B.J., Forsyth P.A., Noyes D., Adeegbe D.O., Eroglu Z., Nguyen K.T., Tsai K.Y., Rix U., Burd C.E., Chen Y.A., Rodriguez P.C, & Smalley K.S. (2021). Targeted therapy given after anti-PD-1 leads to prolonged responses in mouse melanoma models through sustained antitumor immunity. Cancer immunology research, 9(5), 554-567.
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