Blood ammonia assay kit

The serum ammonia content was determined using a blood ammonia assay kit according to the manufacturer's instruction (Nanjing Jiancheng Bioengineering Institute, China).

After ALF induction, all monkeys were monitored every 12 h for the first 48 h and every 24 h for the remainder of the study. During the 6 h treatment, animals were monitored every hour (Figure 1B). Vital signs were recorded by cardiogram monitoring. Blood samples were collected for subsequent analyses. Serum biochemistry was assayed using a chemistry analyzer AU5800 series (Beckman Coulter). Ammonia concentrations were quantified by a blood ammonia assay kit (Nanjing Jiancheng, Nanjing, China). S-100 β proteins have emerged as a biomarker of blood-brain barrier (BBB) permeability and neuropathological conditions including encephaledema and increased ICP 13 (link), 14 (link). Thus, elevated S-100 β levels were measured using an ELISA kit (Ruikesi, Chengdu, China). Rhesus IgG and IgM levels were detected using ELISA kits (Ruikesi). All the kits were analyzed with a MQX 200 microplate reader (BioTek Instruments Inc., VT, US). Cytokines were assessed using a Luminex 100 instrument with xPONENT 3.1 software using Monkey Cytokine Magnetic 29-Plex Panel (Invitrogen, CA, US).