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Molecular biology reagents

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Molecular biology reagents are a collection of chemicals, buffers, and solutions used in various molecular biology techniques. These reagents are designed to facilitate the manipulation and analysis of DNA, RNA, and proteins. They provide the necessary components for processes such as DNA extraction, amplification, purification, and modification. The core function of these reagents is to support fundamental molecular biology research and applications.

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Lab products found in correlation

Coelenterazine 400a, by Biotrend (2 mentions) Mouse anti tubulin antibody, by Merck Group (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Oligonucleotide, by Integrated DNA Technologies (2 mentions) Rabbit anti pfg377, by Merck Group (2 mentions) Mouse anti pfp230p, by Merck Group (2 mentions) Isoproterenol, by Merck Group (1 mentions) 125i iodopindolol, by PerkinElmer (1 mentions) Pindolol, by Merck Group (1 mentions) Dntps, by Thermo Fisher Scientific (1 mentions) Taqman genotyping assay, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Thermo Fisher Scientific (1 mentions) Taq polymerase, by Thermo Fisher Scientific (1 mentions) Rabbit anti ha, by Santa Cruz Biotechnology (1 mentions) Rabbit anti gapdh, by Santa Cruz Biotechnology (1 mentions) Oligonucleotide, by Merck Group (1 mentions) Restriction enzyme, by New England Biolabs (1 mentions) Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions) Trypsin, by Merck Group (1 mentions) Bapta am, by Merck Group (1 mentions)

Close spelling variants of this product

Molecular biology grade reagents and chemicals (4 citations) Chemical reagents for molecular biology (2 citations) Molecular biology-grade reagents (2 citations) Molecular biology reagent grade (2 citations) Molecular biology reagent water (2 citations)

6 protocols using molecular biology reagents

1

Molecular Biology Reagents and Assays

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Molecular biology reagents, as well as the tissue culture media and reagents, were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Gibco Invitrogen Corporation (Breda, the Netherlands), unless otherwise specified. [125I]-Iodopindolol was obtained from PerkinElmer (Boston, MA, USA). The ligands pindolol and isoproterenol were purchased from Sigma-Aldrich, and recombinant human insulin S100 was from Novo Nordisk A/S (Bagsværd, Denmark). Coelenterazine 400a was purchased from Biotrend Chemikalien GmbH (Köln, Germany). Anti-hemagglutinin (HA) high-affinity rat monoclonal antibodies were from Roche (Basel, Switzerland). Anti-rat horseradish peroxidase (HRP)-conjugated antibodies and anti-rat TRITC-conjugated antibodies were from Sigma-Aldrich.
Mandić M., Drinovec L., Glisic S., Veljkovic N., Nøhr J, & Vrecl M. (2014). Demonstration of a Direct Interaction between β2-Adrenergic Receptor and Insulin Receptor by BRET and Bioinformatics. PLoS ONE, 9(11), e112664.
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2

SNP Genotyping Using TaqMan Assay

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Genotyping of SNPs was carried out using the TaqMan genotyping assay (Applied Biosystems, Foster City, CA). The GTxpress Master Mix was used to detect the SNP present. The Master Mix included 10× mango buffer, nucleotides dNTPs, MgCl2, Taq polymerase, Primer-F, Primer-R wt 400bbt (+), Primer-R ins 194b (−), and H2O (Applied Biosystems)[31 (link)–35 (link)]. The assay part number for the TaqMan probes was 4331349 for PNPLA3 (rs738409, rs6006460), for APOC3 (rs2854116, rs2854117), for GCKR (rs780094, rs741038), for FABP2 (rs1799883), for PEMT (rs7946, rs12325817), and for LYPLAL1 (rs12137855).
The total experimental volume for each sample was 10.05 μl per reaction, which was composed of 4.8 μl of the GTxpress Master Mix, 0.25 μl of the TaqMan genotyping assay, 3 μl molecular biology reagents (Sigma Life Science), and 2 μl DNA. Amplification was carried out by the 7500 Fast Real-Time PCR Machine under the following conditions: 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for1 min until completion.
Garner C.J., Conn C.A., Cohen D., Luo L., Castillo J.J., Shah V.O, & Garver W.S. (2015). NAFLD Susceptibility Genes and their Association with Type 2 Diabetes and Obesity in a New Mexico Population. Journal of diabetes and obesity, 2(2), 10.15436/2376-0494.15.024.
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3

Detailed Protocol for Malaria Invasion Assay

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All the molecular biology reagents were purchased from Sigma-Aldrich. Oligonucleotides were procured form Sigma-Aldrich, restriction enzymes were obtained from New England Biolabs, USA. Commercially available antibodies were obtained from Santa Cruz Biotechnology (mouse anti- β-Actin (1:500, C4, sc-47778 HRP), rabbit anti-HA (1:500, Y-11, sc-805) and rabbit anti-GAPDH (1:500, FL 335, sc-25778)). Protein A/G plus agarose beads (sc-2003) were obtained from Santa Cruz Biotechnology. Anti-EBA-175 antibody was obtained from MR4 (1 : 1000, MRA-2). Other antibodies were kindly gifted by Dr. Chetan Chitnis, Institute Pasteur, Paris (1 : 1000, rabbit anti-AMA1), Prof. Alan Cowman, Walter & Eliza Hall Institute (1 : 2500, rabbit anti-RH5), Dr. Anthony A. Holder (1 : 2000 rabbit anti-GAP45). Shld-1 was purchased from Cheminpharma, LLC, USA. PKA Inhibitor PKI was obtained from Enzo life sciences (PKI14-22 amide, myristoylated), 8Br-cAMP, BAPTA-AM, Neuraminidase, trypsin, chymotrypsin were obtained from Sigma-Aldrich.
Kumar S., Kumar M., Ekka R., Dvorin J.D., Paul A.S., Madugundu A.K., Gilberger T., Gowda H., Duraisingh M.T., Keshava Prasad T.S, & Sharma P. (2017). PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum. Nature Communications, 8, 63.
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4

Radioligand Binding and Immunostaining Assays

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Molecular biology reagents, tissue culture reagents, and media were from Sigma-Aldrich (St. Louis, MO, USA) and Gibco Invitrogen Corporation (Breda, The Netherlands). [125I]-iodosulpride was purchased from PerkinElmer (Boston, MA, USA). Dopamine and (S)-(−)-sulpiride were obtained from Sigma-Aldrich and coelenterazine 400a from Biotrend Chemikalien GmbH (Köln, Germany). Anti-hemagglutinin (HA) rat monoclonal and anti-calnexin rabbit polyclonal antibodies were from Roche (Basel, Switzerland) and Sigma-Aldrich, respectively. Anti-rat horseradish peroxidase (HRP)-conjugated, anti-rat fluorescein isothiocyanate (FITC)-conjugated and anti-rabbit tetramethylrhodamine (TRITC)-conjugated antibodies were purchased from Sigma-Aldrich.
Kubale V., Blagotinšek K., Nøhr J., Eidne K.A, & Vrecl M. (2016). The Conserved Arginine Cluster in the Insert of the Third Cytoplasmic Loop of the Long Form of the D2 Dopamine Receptor (D2L-R) Acts as an Intracellular Retention Signal. International Journal of Molecular Sciences, 17(7), 1152.
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5

Reagents and Antibodies for Malaria Research

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All the molecular biology reagents were from MilliporeSigma (USA) until otherwise stated. All the oligonucleotides were purchased from Integrated DNA Technologies. The following primary antibodies and antisera and dilutions were used: mouse anti-tubulin antibody (1:250; Sigma-Aldrich; catalog no. T5168), rabbit anti-Pfg377 (1:250; kindly gifted by Pietro Alano at Istituto Superiore di Sanità, Italy), and mouse anti-PfP230p (1:200, kindly gifted by Kim C. Williamson, Uniformed Services University of the Health Sciences, USA) (94 (link)). The rabbit anti-PfUIS4 (1:100) is described elsewhere (95 (link)). The generation of polyclonal mouse antisera against PfSRPK1 (1:50) is described below. Alexa Fluor-conjugated secondary antibodies were purchased from Thermo Scientific.
Kumar S., Baranwal V.K., Leeb A.S., Haile M.T., Oualim K.M., Hertoghs N., Vaughan A.M, & Kappe S.H. (2022). PfSRPK1 Regulates Asexual Blood Stage Schizogony and Is Essential for Male Gamete Formation. Microbiology Spectrum, 10(5), e02141-22.
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6

Reagents and Antibodies for Malaria Research

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All the molecular biology reagents were from MilliporeSigma (USA) until otherwise stated. All the oligonucleotides were purchased from Integrated DNA Technologies. The following primary antibodies and antisera and dilutions were used: mouse anti-tubulin antibody (1:250; Sigma-Aldrich; catalog no. T5168), rabbit anti-Pfg377 (1:250; kindly gifted by Pietro Alano at Istituto Superiore di Sanità, Italy), and mouse anti-PfP230p (1:200, kindly gifted by Kim C. Williamson, Uniformed Services University of the Health Sciences, USA) (94 (link)). The rabbit anti-PfUIS4 (1:100) is described elsewhere (95 (link)). The generation of polyclonal mouse antisera against PfSRPK1 (1:50) is described below. Alexa Fluor-conjugated secondary antibodies were purchased from Thermo Scientific.
Kumar S., Baranwal V.K., Leeb A.S., Haile M.T., Oualim K.M., Hertoghs N., Vaughan A.M, & Kappe S.H. (2022). PfSRPK1 Regulates Asexual Blood Stage Schizogony and Is Essential for Male Gamete Formation. Microbiology Spectrum, 10(5), e02141-22.
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