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Anti cleaved caspase 3 9662

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-cleaved caspase 3 (9662) is a primary antibody that detects the cleaved form of caspase-3 protein. Caspase-3 is a key effector of apoptosis, or programmed cell death. This antibody recognizes the p17 fragment of activated caspase-3 and can be used to monitor apoptosis in cells and tissues.

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2 protocols using anti cleaved caspase 3 9662

1

Immunoblotting Antibodies for Focal Adhesion Kinase

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Monoclonal mouse anti-FAK (4.47, 05-0537) and anti-phospho Src (Tyr 416, 05-677) antibodies were obtained from Millipore (EMD Millipore, Billerica, MA) and rabbit polyclonal anti-phospho-FAK (Y397, 71-7900) and anti-phospho Erk 1/2 (05-797R) antibodies were from Invitrogen (Invitrogen Corp., Carlsbad, CA). Rabbit polyclonal anti-PARP (9542S), anti-Akt (9272), anti-phospho Akt (Ser 473, 9271), anti-Erk 1/2 (9102) and anti-cleaved caspase 3 (9662) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Monoclonal mouse anti-Jnk (F-3, sc-1648), anti-phospho Jnk (G-7, sc-6254) and rabbit polyclonal anti-c-Src (sc-18) were from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal mouse anti-GAPDH was from Millipore (MAB374) and anti-β-actin was from Sigma (A1978, Sigma-Aldrich Corp., St. Louis, MO). The small molecule PF-573,228 (C22H20F3N5O3S) was obtained from Pfizer (New York, NY), and the small molecule 1,2,4,5-benzenetetraamine tetrahydrochloride (Y15) (C6H10N4·4ClH) from Sigma.
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2

Characterizing Apoptotic Pathway Proteins

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The anti‐TRAIL antibody (sc‐7877) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA), and the anti‐DR5 antibody (PX064A) was obtained from Cell Sciences (Canton, MA, USA). The anti‐β‐actin (#4970), anticleaved caspase 8 (#8592), and anticleaved caspase 3 (#9662) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Recombinant murine TRAIL/TNFSF10 (1121‐TL‐010) and OPG (459‐MO‐100) were from R & D systems (Minneapolis, MN, USA), and MG132 was from Sigma‐Aldrich (C2211, St. Louis, MO, USA).
Riboprobe combination system‐T3/T7 was from Promega (Madison, WI, USA). In situ hybridization solutions were from Roche (Basel, Switzerland), and 1‐step NBT/BCIP Plus suppressor solution was from Thermo Scientific (Cambridge, MA, USA).
The VOT‐33 cell line, a conditionally immortal cell line derived from an embryonic mouse cochlear neuroblast, was a gift provided by Dr. Matthew Holley.
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