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Anti acetyl histone h3 antibody 06 599

Manufactured by Merck Group

The Anti-acetyl-histone H3 antibody (06-599) is a laboratory reagent produced by Merck Group. It is designed to detect acetylated histone H3 in various experimental applications.

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2 protocols using anti acetyl histone h3 antibody 06 599

1

Antibody Sources for Immunoblotting

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A monoclonal antibody against E-selectin (clone 7A9) was obtained from the American Type Culture Collection; anti-E-selectin (A-10: sc-137203) and anti-NF-kB p65 (C-20: sc-372) antibodies were obtained from Santa Cruz Biotechnology; anti-phospho-NF-κB p65 Ser 536 (#3031), anti-SAPK/JNK (#9252), anti-p38 MAPK (#9212), anti-phospho-SAPK/JNK (#9251), and anti-phospho-p38 MAPK (#4511) antibodies were obtained from Cell Signaling Technology; anti-lamin A/C (SAB4200236) and anti-actin (A5060) antibodies were obtained from Sigma-Aldrich; an anti-α-tubulin antibody (PM054-7) was obtained from Medical & Biological Laboratories; an anti-acetyl-histone H3 antibody (06-599) was obtained from Millipore; an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (A11017) was obtained from Life Technologies; horseradish peroxidase (HRP)-linked anti-mouse (NA931V) and anti-rabbit (NA9340V) secondary antibodies were obtained from GE Healthcare.
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2

Protein Expression Analysis by Western Blot

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Lysates prepared from either cultured cell lines or ex vivo sorted cells were Western-blotted with anti-Pax5 antibody 1H9, anti-GFP antibody A6455 (Invitrogen), and anti-acetyl-Histone H3 antibody 06-599 (Millipore).
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