Cauda epididymal spermatozoa were dispersed in TYH (Toyoda, Yokoyama, Hoshi) drops for sperm motility. After an incubation period of 10 and 120 min, the sperm motility pattern was examined using the CEROS II sperm analysis system (software version 12.3; HamiltonThorne Biosciences) [22 (link)] and the default program (Mouse CytoD ×4 dark-field settings) of the CEROS II sperm analysis system (software version 1.5.2; Hamilton Thorne Biosciences).
Ceros 2 sperm analysis system
Manufactured by Hamilton Thorne
Sourced in United States
The CEROS II is a sperm analysis system developed by Hamilton Thorne. It is designed to objectively and accurately measure various parameters of sperm samples, including motility, concentration, and morphology. The system employs advanced technology to provide reliable and consistent results for use in research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
Bx53 microscope, by Olympus (6 mentions)
Mas coated glass slide, by Matsunami (4 mentions)
Bx53 differential interference contrast microscope, by Olympus (1 mentions)
Mayer s hematoxylin solution, by Fujifilm (1 mentions)
Periodic acid, by Nacalai Tesque (1 mentions)
Dp74 color camera, by Olympus (1 mentions)
Schiff s reagent, by Fujifilm (1 mentions)
Ceros sperm analysis system, by Hamilton Thorne (1 mentions)
Jem 1410, by JEOL (1 mentions)
Axiolab 5, by Zeiss (1 mentions)
17 protocols using ceros 2 sperm analysis system
1
Evaluating Epididymal Sperm Motility
2
Comprehensive Male Mouse Reproductive Analysis
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After the fertility test, three knockout male mice and three same-aged wild-type B6D2F1/J mice were euthanized by cervical dislocation following anesthesia to examine their testis weights, testicular and epididymal histology, sperm morphology, and sperm motility. Testes and epididymides were fixed in Bouin fluid (Polysciences, Inc., Warrington, PA), embedded in paraffin wax, sectioned at a thickness of 5 μm on a Microm HM325 microtome (Microm, Walldorf, Germany), and stained with 1% periodic acid (Nacalai Tesque, Kyoto, Japan) and Schiff's reagent (Wako, Osaka, Japan) followed by counterstaining with Mayer's hematoxylin solution (Wako, Osaka, Japan). Spermatozoa were extracted from cauda epididymis and dispersed in TYH medium for 10 min. Sperm morphology was observed under an Olympus BX53 differential interference contrast microscope equipped with an Olympus DP74 color camera (Olympus, Tokyo, Japan). Sperm motility was measured with the CEROS II sperm analysis system (Hamilton Thorne Biosciences, Beverly, MA) at 10 min and 2 h of incubation.
3
Sperm Velocity Analysis Protocol
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Sperm velocity was analyzed as described previously (Miyata et al., 2015 (link); Morohoshi et al., 2020 (link)). Cauda epididymal spermatozoa were dispersed in a drop of TYH medium. Sperm velocity was measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences) for Fam71f1 or CEROS II sperm analysis system (software version 1.4; Hamilton Thorne Biosciences) for Fam71f2 at 10 min and 2 h after incubation at 37°C under 5% CO2. More than 200 spermatozoa were analyzed for each male.
4
Sperm Motility Analysis in TYH Medium
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Sperm motility analysis was conducted as described previously (Miyata et al., 2021 (link)). Cauda epididymal spermatozoa were suspended and incubated in TYH medium, which can induce sperm capacitation (Toyoda and Yokoyama, 2016 (link)). Sperm motility was then measured using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences). The motility of epididymal spermatozoa was recorded after 10 min and 2 h of incubation in TYH medium.
5
Sperm Morphology and Motility Analysis
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Spermatozoa from the cauda epididymis were suspended in a 100 µL drop of TYH medium [13 (link)] and incubated at 37°C under 5%
CO2. For observing sperm morphology, spermatozoa were placed on MAS coated glass slides (Matsunami Glass, Osaka, Japan) and observed using an Olympus BX53 microscope (Tokyo,
Japan). For analyzing sperm motility, spermatozoa were collected from the top of the drop after 10 or 120 min incubation and were analyzed using the CEROS II sperm analysis system (software
version 1.5; Hamilton Thorne Biosciences, Beverly, MA, USA). For analyzing sperm flagellar waveforms, spermatozoa were observed with an Olympus BX-53 microscope equipped with a high-speed
camera (HAS-L1, Ditect, Tokyo, Japan) [5 (link), 14 (link)]. The motility was videotaped at 200 frames per second or 50 frames
per second. Waveforms were traced using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan) [15 (link)].
CO2. For observing sperm morphology, spermatozoa were placed on MAS coated glass slides (Matsunami Glass, Osaka, Japan) and observed using an Olympus BX53 microscope (Tokyo,
Japan). For analyzing sperm motility, spermatozoa were collected from the top of the drop after 10 or 120 min incubation and were analyzed using the CEROS II sperm analysis system (software
version 1.5; Hamilton Thorne Biosciences, Beverly, MA, USA). For analyzing sperm flagellar waveforms, spermatozoa were observed with an Olympus BX-53 microscope equipped with a high-speed
camera (HAS-L1, Ditect, Tokyo, Japan) [5 (link), 14 (link)]. The motility was videotaped at 200 frames per second or 50 frames
per second. Waveforms were traced using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan) [15 (link)].
6
Sperm Motion Analysis and Morphology
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Cauda epididymal spermatozoa were dispersed in TYH medium82 (link) for analyzing sperm motility. At 10 and 120 min after the sperm incubation, velocity parameters were examined by using the CEROS II sperm analysis system (software version 1.5.2; Hamilton Thorne Biosciences, Massachusetts, USA). For analyzing flagellar bending patterns, sperm motility was videotaped at 200 frames per second using an Olympus BX-53 microscope equipped with a high-speed camera (HAS-L1, Ditect, Tokyo, Japan) as described previously83 (link). Obtained frame images were analyzed using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan)84 (link). Single frames throughout one beating cycle were superimposed; in abnormal (bent or broken mid piece) spermatozoa with unclear beating cycle, 20 frames were superimposed.
7
Analyzing Sperm Morphology and Motility
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Spermatozoa from cauda epididymis were suspended in Toyoda, Yokoyama, Hoshi (TYH) medium19 and placed on MAS‐coated glass slides (Matsunami Glass) for morphology assessment using an Olympus BX53 microscope. For analyzing sperm motility, spermatozoa were incubated in TYH medium for 10 or 120 min and placed in glass chambers (Leja). Sperm motility was analyzed as previously described20 using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences).
8
Spermatozoa Motility and Morphology Assessment
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Spermatozoa from cauda epididymis were suspended in TYH medium,26 (link) incubated at 37°C for either 10 min or 120 min, diluted, and then placed on MAS-coated glass slides (Matsunami Glass, Osaka, Japan) for morphology assessment using an Olympus BX53 microscope or placed in glass chambers for sperm motility analysis using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences, Beverly, MA, USA).
9
Sperm Motility Measurement Technique
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Cauda epididymal spermatozoa were suspended in TYH medium [21 , 22 ]. Sperm velocity was measured using the CEROS II sperm analysis system (Hamilton Thorne Biosciences, Beverly, MA) at 10 min and 2 h after incubation. More than 200 spermatozoa were analyzed for each male.
10
Sperm Motility Analysis Protocol
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Sperm motility analysis was performed as previously described [28 (link)]. Cauda epididymal spermatozoa were suspended and incubated in TYH medium that can
induce sperm capacitation [25 (link)]. Spermatozoa were loaded onto prewarmed 100 µm deep Leja counting chamber slides (Leja, Nieuw-Vennep,
The Netherlands) for analysis. Sperm motility was then measured using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences, Beverly, MA, USA). The motility
of epididymal spermatozoa was recorded after 10 min and 2 h of incubation in TYH medium.
For waveform tracing, spermatozoa were observed with an Olympus BX53 microscope equipped with a high-speed camera (HAS-L1, Ditect, Tokyo, Japan). The motility was videotaped at 200 frames
per second and analyzed for waveforms using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan) [29 (link)].
induce sperm capacitation [25 (link)]. Spermatozoa were loaded onto prewarmed 100 µm deep Leja counting chamber slides (Leja, Nieuw-Vennep,
The Netherlands) for analysis. Sperm motility was then measured using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences, Beverly, MA, USA). The motility
of epididymal spermatozoa was recorded after 10 min and 2 h of incubation in TYH medium.
For waveform tracing, spermatozoa were observed with an Olympus BX53 microscope equipped with a high-speed camera (HAS-L1, Ditect, Tokyo, Japan). The motility was videotaped at 200 frames
per second and analyzed for waveforms using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan) [29 (link)].
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