The following cells were obtained from RIKEN (Wako, Japan): HL-60, Jurkat, K562, and THP-1. Cells were grown in RPMI1640 medium (Wako Pure Chemical) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Corning, NY, USA), 100 µg/mL streptomycin, and 100 units/mL penicillin. Cells were cultured under standard conditions at 37 °C in a humidified incubator containing 5% CO2. To allow for differentiation into neutrophils, HL-60 cells were collected by brief centrifugation, resuspended in RPMI medium containing 1.3% DMSO at 1 × 105 cells/mL, and cultured for 3 days. Cell numbers were determined using a Z1 Coulter particle counter (Beckman Coulter, CA, USA).
Hl 60
Manufactured by Fujifilm
Sourced in Japan
The HL-60 is a multipurpose laboratory centrifuge designed for a variety of applications. It features a compact and durable construction, accommodating a range of rotor and sample types. The HL-60 is capable of processing samples at high speeds, enabling efficient separation and extraction procedures.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Fujifilm (2 mentions)
Rpmi 1640, by Fujifilm (2 mentions)
Z1 coulter particle counter, by Beckman Coulter (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Hl 60, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Thp 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Katoiii, by Fujifilm (1 mentions)
Takara pcr mycoplasma detection set, by Takara Bio (1 mentions)
Nugc3, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
L glutamine, by Fujifilm (1 mentions)
Penicillin streptomycin, by Fujifilm (1 mentions)
Panc 1, by RIKEN BioResource Center (1 mentions)
Hgc 27, by RIKEN BioResource Center (1 mentions)
Katoiii, by RIKEN BioResource Center (1 mentions)
P brca1, by Santa Cruz Biotechnology (1 mentions)
Hl 60, by American Type Culture Collection (1 mentions)
I bet151, by Selleck Chemicals (1 mentions)
5 azacytidine, by Fujifilm (1 mentions)
5 protocols using hl 60
1
Cell Culture and Neutrophil Differentiation
2
Cell Line Culture Protocols for Gastric and Lung Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human GC cell lines HGC27, GSU, MKN1, and KATOIII, a human immortalized fetal lung fibroblast cell line L23immo, and a human pancreatic carcinoma cell line Panc-1 were obtained from RIKEN BioResource Research Center (BRC) (Japan). A human GC cell line NUGC-3, a human monocytic leukemia cell line THP-1, a human promyelocytic leukemia cell line HL60, and a human lung adenocarcinoma cell line A549 were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Japan). Cells were cultured in RPMI1640 medium (#189-02025, FUJIFILM Wako Pure Chemical Corporation, Japan) supplemented with 10% fetal bovine serum (FBS) (#172012, Sigma-Aldrich) (with exceptions of KATOIII and HL60 that were cultured with 20% FBS), 2 mM L-glutamine (#073-05391, FUJIFILM Wako Pure Chemical Corporation), Penicillin/Streptomycin (#168-23191, FUJIFILM Wako Pure Chemical Corporation), and 1 mM sodium pyruvate (#11360-070, Thermo Fisher Scientific). Negativity for Mycoplasma infection has been regularly confirmed using TaKaRa PCR Mycoplasma Detection Set (TaKaRa Bio, Japan).
3
Establishing AZA-Resistant Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
U937 and HL-60 cells were purchased from ATCC (Manassas, VA, United States). AZA-resistant cell lines (R-U937 and R-HL-60) were originally created in our laboratory from U937 and HL-60 cells, respectively (Imanishi et al., 2014 (link)).
5-Azacytidine was purchased from Wako Pure Chemical Industries (Osaka, Japan) and I-BET151 from Selleck Chemicals (Houston, TX, United States). The antibodies specific for HP1α, HP1β, HP1γ, and β-actin were purchased from Abcam (Cambridge, United Kingdom). The antibodies specific for BRCA1 phosphorylated at Ser 1423 (p-BRCA1), total BRCA1 and total ATM were from Santa Cruz Biotechnology (Dallas, TX, United States) and anti ATM phosphorylated at Ser 1981 (p-ATM) antibody was from R&D Systems (Minneapolis, MN, United States). Anti PARP1 antibody was from Cell Signaling Technologies (Danvers, MA, United States). The secondary antibodies, namely horseradish peroxidase–labeled anti-mouse IgG antibody and horseradish peroxidase–labeled anti-rabbit IgG antibody, were purchased from GE Healthcare (Buckinghamshire, United Kingdom).
5-Azacytidine was purchased from Wako Pure Chemical Industries (Osaka, Japan) and I-BET151 from Selleck Chemicals (Houston, TX, United States). The antibodies specific for HP1α, HP1β, HP1γ, and β-actin were purchased from Abcam (Cambridge, United Kingdom). The antibodies specific for BRCA1 phosphorylated at Ser 1423 (p-BRCA1), total BRCA1 and total ATM were from Santa Cruz Biotechnology (Dallas, TX, United States) and anti ATM phosphorylated at Ser 1981 (p-ATM) antibody was from R&D Systems (Minneapolis, MN, United States). Anti PARP1 antibody was from Cell Signaling Technologies (Danvers, MA, United States). The secondary antibodies, namely horseradish peroxidase–labeled anti-mouse IgG antibody and horseradish peroxidase–labeled anti-rabbit IgG antibody, were purchased from GE Healthcare (Buckinghamshire, United Kingdom).
4
Differentiation and C5a Stimulation of HL-60 Leukemia Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human monocytic leukemia cell line (THP‐1) was obtained from RIKEN BioResource Research Center Cell Bank (Tsukuba, Japan) and was maintained in RPMI‐1640 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) culture medium containing 10% fetal bovine serum (FBS), 100 IU/ml penicillin, and 0.17 mmol/L streptomycin (Gibco, Life Technologies Japan, Tokyo, Japan). The human leukemia cell line HL‐60 (ATCC) was cultured in RPMI‐1640 containing 10% FBS and penicillin/streptomycin. To differentiate HL‐60 cells, 1.3% dimethyl sulfoxide (DMSO; FUJIFILM Wako Pure Chemical Corporation) was added to the culture medium, and they were cultured for 5 days in an incubator maintained at 37°C with 5% CO2 [3 , 9 ].
Differentiated HL‐60 (dHL‐60, 1 × 106 cells) cells were stimulated by 3 nm recombinant human C5a (PeproTech Inc. NJ, USA) followed by each experiment.
Differentiated HL‐60 (dHL‐60, 1 × 106 cells) cells were stimulated by 3 n
5
Aclarubicin's Impact on Leukemia Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials. Aclarubicin hydrochloride was purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Superoxide dismutase (SOD; 3,000 U/mg from bovine erythrocytes), catalase (45,000 U/mg from bovine liver) and cytochrome c (from equine heart) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Plasmid DNA (pBR322) and DNA gel loading dye (6×) were from Toyobo Co. (Osaka, Japan). Copper chloride (CuCl 2 •2H 2 O) Cell culture and treatment with ACR. Human leukemia HL-60 and HP100 cells were obtained from Riken BioResource Research Center (Tsukuba, Japan). HP100 cells have approximately 340-fold higher resistance to H 2 O 2 (14) (link) and 18 times higher catalase activity (15) than HL-60 cells. By use of HP100 cells, effects of intracellular catalase can be evaluated (16) (link). HL-60 and HP100 cells were grown in RPMI 1640 (FUJIFILM Wako Pure Chemical Co.) supplemented with 6% fetal bovine serum (Biowest Co., Nuaillé, France) at 37˚C under 5% CO 2 in a humidified atmosphere. The cells (0.5×10 6 or 1×10 6 cells/ml) were then treated with 0.05, 0.1, 0.2 and 0.5 μM of ACR.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!