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Vp series

Manufactured by Shimadzu
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Sourced in Japan

The VP series is a line of laboratory equipment from Shimadzu. The core function of the VP series is to provide reliable and precise measurements for various analytical applications in the laboratory setting.

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Lab products found in correlation

Lc 20ad pump, by Shimadzu (1 mentions) Cosmosil 5c18 ms 2 column, by Nacalai Tesque (1 mentions) Cto 20ac, by Shimadzu (1 mentions) Vp ods c18 column, by Shimadzu (1 mentions) Spd m20a, by Shimadzu (1 mentions) Cbm 20a, by Shimadzu (1 mentions) Phi 5600, by Physical Electronics (1 mentions) Waters symmetry c18 column, by Waters Corporation (1 mentions) Symmetry c18, by Waters Corporation (1 mentions) Lambda 25, by PerkinElmer (1 mentions) Symmetry c18 column, by Waters Corporation (1 mentions)

8 protocols using vp series

1

Quantitative HPLC Analysis of Carbamazepine

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CBZ concentration in the EN or plasma samples was determined by high-performance liquid chromatography (HPLC). One microgram of the internal standard (flunitrazepam) and 50 μL of acetonitrile were added to 50 µL of the samples. The samples were vortexed for 30 s and centrifuged at 11,200 ×g for 20 min at 4 °C. Thereafter, 50 µL of the supernatant was transferred to a clean tube, and 20 µL of the supernatant was injected into the HPLC system.
The HPLC system used was a Shimadzu VP-series consisting of an LC-20AD pump, DGU-20A deaeration unit, CTO-20Avp column oven, SPD-20ADvp UV detector, and SIL-20ADvp auto-injector, controlled by an SCL-20 Avp controller (Shimadzu Co., Kyoto, Japan). Separations were performed on a Cosmosil® 5C18-MS-II column (4.6 mm I.D. × 150 mm; Nacalai Tesque, Inc., Kyoto, Japan), preceded by a run through the Cosmosil® 5C18-MS-II guard column (4.6 mm I.D. × 10 mm). The mobile phase (15 mM phosphate buffer:acetonitrile (25:55 (v/v)) was flowed at 0.8 mL/min. The column temperature was maintained at 40 °C, and eluting peaks were monitored by UV absorbance at 215 nm.
Urashima Y., Kobayashi H., Yamamoto K., Matsushita K., Urashima K., Tsujikawa M., Suzuki K., Kurachi K., Nishihara M., Neo M., Myotoku M., Kobori T, & Obata T. (2022). Liquid Enteral Nutrients Alter the Pharmacokinetics of Orally Administered Carbamazepine in Rats. International Journal of Medical Sciences, 19(5), 789-795.
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2

Identification of Phenolic Compounds in Plant Extracts

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The identification of phenolic compounds was carried out, using a liquid chromatograph Shimadzu (VP series, Kyoto, Japan), a system controller (CBM-20A), a pump (LC-20AT vp) and a column oven (CTO-20A/C). The column used was a Shimadzu VP-ODS C18 column (250 L x4.6 mm), with an UV/vis detector (SPD-M20A) and a computer software (LC-solution). The reversed-phase HPLC analyses were adapted from Glória et al. (2017 (link)). The mobile phase was composed by 0.5 % acetic acid in purified water (solvent A), and acetonitrile (solvent B). The solvent gradient was programmed as: 0’: 100 % A; 5’: 85 % A; 10’: 80 % A; 15’: 70 % A; 20’: 60 % A; 25’: 50 % A; 30’: 70 % A; 40’: 85 % A; 41’: stop. A flow rate of 1.0 mL min−1 and a column temperature of 36 °C. STPE and STWFE extracts were dissolved in the mobile phase (5 mg mL−1) and filtered through a 0.45 μm nylon membrane, prior to HPLC injection. The injection volume was 20 μL, using a detection wavelength of 280 nm. The phenolic compounds present in the extracts were identified by comparing the retention times (Rt) of the standards, as well as by co-injection of the samples with standards. The standards were dissolved in the mobile phase to reach a concentration of 0.5 mg mL−1. The phenolic standards were analysed using the same conditions of the extract.
Oliveira M.B., Valentim I.B., Rocha T.S., Santos J.C., Pires K.S., Tanabe E.L., Borbely K.S., Borbely A.U, & Goulart M.O. (2020). Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant. Industrial Crops and Products, 152, 112503.
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3

HPLC Quantification of CBD in Nanoparticles

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To quantify CBD (within nano-sized particles), to about 25 μL aliquots from each particle sample that was carefully weighed, 975 μL MeOH was added and stirred. After at least 10 min, the samples were further diluted 1:10, and then the liquid was injected into an HPLC system (Shimadzu VP series, Shimadzu Corp., Tokyo, Japan), equipped with a prepacked column (ReproSil-Pur 300 ODS-3, 5 µm, 250 mm 4.6 mm, Dr. Maisch, Ammerbuch, Germany), which was constantly maintained at 30 °C. The samples were chromatographed using a mobile phase of acetonitrile-35 mM acetic acid (75:25) at a 1 mL/min flow rate. A calibration curve, peak area measured at 208 nm versus CBD concentration, was constructed by running standard drug solutions in MeOH for each series of chromatographed samples.
Zamansky M., Yariv D., Feinshtein V., Ben-Shabat S, & Sintov A.C. (2023). Cannabidiol-Loaded Lipid-Stabilized Nanoparticles Alleviate Psoriasis Severity in Mice: A New Approach for Improved Topical Drug Delivery. Molecules, 28(19), 6907.
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4

Lutein Determination in Egg Yolks

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On d 28, 10 eggs were randomly selected from each of the CON and ME groups, and the yolks were separated and stored at -20°C until lutein determination. A high-performance liquid chromatograph (VP series, Shimadzu, Kyoto, Japan) was used to determine the lutein content of egg yolk with reference to the national standard GB-5009.248-2016. In short, a certain amount of egg yolk was weighed, added to dibutylhydroxytoluene, ethanol and sodium hydroxide solution and then shaken and mixed. It was subsequently saponified at room temperature, protected from light for 30 min, extracted, washed, concentrated, and passed through a 0.45 μm filter membrane for measurement. Chromatographic separation was performed on a C30 column (5 μm, 250 mm × 4.6 mm) at 30°C with the mobile phase methanol : water = 88:12, gradient elution, a flow rate of 1 mL/min, an injection volume of 50 μL, and a detection wavelength of 445 nm.
Chen R., Jiang C., Li X., Shi X., Zhuang L., Zhou W., Zhou C., Xuan L., Xu G, & Zheng J. (2023). Research on Chinese consumers' shell egg consumption preferences and the egg quality of functional eggs. Poultry Science, 102(10), 103007.
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5

Characterization of g-C3N4 Photocatalyst

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The concentrations of free chlorine were determined by a DPD colorimetric method. 39 Concentrations of CBZ, NB, BA and DMOB were determined by a reverse phase UPLC (VP series, Shimadzu) equipped with a Waters symmetry C18 column (4.6 mm × 150 mm, 5 μM particle size; Waters) and a UV detector. The flow rate was 1.0 mL/min and the mobile phase was a mixture of water (pH 3, adjusted using phosphoric acid) and methanol (55/45, v/v %). 40 The detection limits of CBZ, NB and BA are 0.06, 0.10, 0.04 and 0.13 μM, respectively.
Before and after reactions the g-C3N4 was subjected to material characterization. The surface morphology of the g-C3N4 samples were characterized by SEM. The elemental composition of the g-C3N4 were obtained by XPS (PHI 5600, Physical Electronics Inc., Al Kα radiation (1486.8 eV), USA). The optical properties of the g-C3N4 were characterized by UV-Vis diffuse reflection spectroscopy (UV-vis DRS, PerlomElmer Lambda 950, USA).
Cheng Z., Ling L., Wu Z., Fang J., Westerhoff P, & Shang C. (2020). Novel Visible Light-Driven Photocatalytic Chlorine Activation Process for Carbamazepine Degradation in Drinking Water. Environmental science & technology, 54(18).
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6

Quantification of Organohalogen Compounds in Water

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The concentrations of NB, BA, DMOB, and benzaldehyde were determined using an ultra-performance liquid chromatograph (UPLC) (VP series, Shimadzu) equipped with a Waters symmetry C18 column and a UV-Vis detector. Eluents of water (pH 2.0, adjusted using phosphoric acid) and methanol (50:50, v/v %) were used to separate NB, BA, and DMOB at a flow rate of 1.0 mL/min. Eluents of water (0.1% phosphoric acid) and acetonitrile (70:30, v/v %)
were used to separate benzaldehyde at a flow rate of 1.0 mL/min. The concentrations of ClO2 -, ClO3 -, and ClO4 -were determined using ion chromatography (IC). The absorption spectra of chlorine and ClO2 -were measured using a UV-Vis spectrophotometer (Lambda 25, Perkin Elmer Inc., USA) with a quartz cuvette providing a light path of 1.0 cm. Details about DBP analysis was described in Text S3.
Zhao J., Shang C., Zhang X., Yang X, & Yin R. (2021). The multiple roles of chlorite on the concentrations of radicals and ozone and formation of chlorate during UV photolysis of free chlorine. Water research, 190.
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7

HPLC Quantification of CBD in Nanoparticles

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To quantify CBD (within nano-sized particles), to about 25 μL aliquots from each particle sample that was carefully weighed, 975 μL MeOH was added and stirred. After at least 10 min, the samples were further diluted 1:10, and then the liquid was injected into an HPLC system (Shimadzu VP series, Shimadzu Corp., Tokyo, Japan), equipped with a prepacked column (ReproSil-Pur 300 ODS-3, 5 µm, 250 mm 4.6 mm, Dr. Maisch, Ammerbuch, Germany), which was constantly maintained at 30 °C. The samples were chromatographed using a mobile phase of acetonitrile-35 mM acetic acid (75:25) at a 1 mL/min flow rate. A calibration curve, peak area measured at 208 nm versus CBD concentration, was constructed by running standard drug solutions in MeOH for each series of chromatographed samples.
Zamansky M., Yariv D., Feinshtein V., Ben-Shabat S, & Sintov A.C. (2023). Cannabidiol-Loaded Lipid-Stabilized Nanoparticles Alleviate Psoriasis Severity in Mice: A New Approach for Improved Topical Drug Delivery. Molecules, 28(19), 6907.
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8

HPLC and GC Analysis of Organic Compounds

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BBMP, NB and phenol concentrations were analyzed by using a high-performance liquid chromatography (HPLC) system (VP series, Shimadzu) with a symmetry C18 column (4.6 mm × 150 mm, 5 μm in particle size; Waters) and a UV-Vis detector. The reference wavelengths were set at 210, 262 and 275 nm for BBMP, NB and phenol respectively. The mobile phases were water/acetonitrile (70:30, v/v %), water/acetonitrile (50:50, v/v %) and water/methanol (55:45, v/v%), respectively, at a flow rate of 1.0 mL/min. The DBP concentrations were measured according to the USEPA Method 551.1. The DBPs in the samples were extracted using the following procedure: 3 mL of methyl-tert-butyl-ether and 8 g of muffled sodium sulfate were added sequentially into the quenched solution and then the vial was sealed with a Teflon-lined cap. After 4-min mixing by vortex shaking followed by 20min stabilization, the separated upper methyl-tert-butyl-ether layer was transferred to a vial for subsequent analysis by gas chromatography. The detailed procedures for the gas chromatography setup are described in a previous report (Yin et al., 2018b) (link). The concentration of the released Cu 2+ in solutions was determined using inductively coupled plasma optical emission spectrometry (ICP-OES).
Yin R., Ling L., Lu S., Li H., Li C, & Shang C. (2020). Degradation of aliphatic halogenated contaminants in water by UVA/Cu-TiO2 and UVA/TiO2 photocatalytic processes: Structure-activity relationship and role of reactive species. Chemosphere, 260.
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