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12 protocols using cobalt chloride hexahydrate

1

In Vitro Digestion of Hericium erinaceus

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Hericium erinaceus was purchased from the Yongding Ecological Farming Family Farm, Ningqiang County, Hanzhong City, Shaanxi Province.
Saliva α amylase (2000 U/g) was obtained from Omega, Cheyenne, WY, USA. Pepsin (250 U/g), pancreatic enzymes (10 U/g), fructose oligosaccharide, salt acid, concentrated sulfuric acid, sodium hydroxide, sodium chloride, ferrous sulfate heptahydrate, manganese sulfate, zinc sulfate heptahydrate, cobalt chloride hexahydrate, nickel chloride, copper sulfate pentahydrate disodium phosphate, potassium chloride, urea, magnesium chloride hexahydrate, calcium chloride dihydrate, metaphosphate, sodium sulfate, acetic acid standard, propionic acid standard, and butyric acid standard were obtained from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China. All other chemical reagents were of analytical grade and procured from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China).
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2

Synthesis of Transition Metal Nanostructures

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All chemicals were of analytical grade and used directly without any pre-treatment. Nickel chloride hexahydrate (NiCl2·6H2O), cobalt chloride hexahydrate (CoCl2·6H2O), ethylene glycol (EG), sodium hydroxide (NaOH), hydrazine hydrate (N2H4·H2O, 85%), ammonium hydroxide solution (28 wt%), tetraethyl orthosilicate (TEOS), tetrabutyl orthotitanate (TBOT), acetonitrile and ethanol were all purchased from Sinopharm Chemical Reagent Company.
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3

Synthesis of Transition Metal Nanostructures

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Iron chloride hexahydrate (FeCl3·6H2O), nickel chloride hexahydrate (NiCl2·6H2O), cobalt chloride hexahydrate (CoCl2·6H2O), chromium chloride hexahydrate (CrCl3·6H2O), sodium borohydride (NaBH4) and ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd. Potassium hydroxide (KOH, electronic grade, 99.999% metals basis, except sodium) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Ruthenium dioxide (99.9% trace metals basis) and Nafion®117 solution (5%) were purchased from Sigma-Aldrich. Carbon black (ECP600JD, Ketjenblack®) for electrochemical measurements was obtained from Suzhou Sinero Technology Co., Ltd. and graphite powder (XF009 7782-42-5) for XAS characterization was obtained from XFNANO Materials Tech Co., Ltd. All chemicals can be used without further purification.
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4

Synthesis and Characterization of Pt-Based Catalysts

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Carbon black (Black Pearls 2000, BP2000) was produced by America Cabot Corporation. Commercial Pt/C (TEC10E20E) was purchased from TANAKA. All others chemicals were commercially available from Sinopharm Chemical Reagent Co. Ltd., China, including chloroplatinic acid (H2PtCl6·6H2O), cobalt chloride hexahydrate (CoCl2·6H2O), copper chloride dehydrate (CuCl2·2H2O), cickel chloride hexahydrate (NiCl2·6H2O), ethanol, and isopropanol. All the chemicals were used as received without further purification. DI water (18.2 MΩ/cm) used in all experiments was prepared by passing through an ultra-pure purification system.
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5

Synthesis of Metal Chloride Catalysts

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Magnesium chloride hexahydrate (MgCl2·6H2O, 98%), calcium chloride (CaCl2, 96%), manganese chloride tetrahydrate (MnCl2·4H2O, 99%), zinc chloride (ZnCl2, 98%), cobalt chloride hexahydrate (CoCl2·6H2O, 99%), nickel chloride hexahydrate (NiCl2·6H2O, 98%), cadmium chloride (CdCl2, 99%), copper chloride dihydrate (CuCl2·2H2O, 98%), stannic chloride pentahydrate (SnCl4·5H2O, 98%), 2-aminoethanol (HO(CH2)2NH2, 99.9%), potassium hydroxide (KOH, 85%), and ethanol (C2H5OH, 99.7%) were obtained from Sinopharm Chemical Reagent Co. Ltd. Hydrogen hexachloroiridate (IV) hexahydrate (H2IrCl6·H2O, 99%) was obtained from Alfa Aesar. Ferrous chloride tetrahydrate (FeCl2·4H2O) was obtained from Strem. Nafion solution (~5 wt.% in a mixture of lower aliphatic alcohols and water) was obtained from Sigma–Aldrich. The carbon powder (XC72R) was purchased from Vulcan. The deionized water (18 MΩ cm−1) used in all experiments was prepared by passing water through an ultrapure purification system.
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6

Synthesis of Cobalt-based Nanoparticles

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Cobalt chloride hexahydrate (CoCl2 · 6H2O), sodium borohydride (NaBH4), sodium citrate dehydrate, and anhydrous ethanol were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd., China. TEOS and 3-aminopropyltriethoxysilane (APS) were purchased from Sigma-Aldrich, St. Louis, MO, USA. All reagents were used as received. Deionized water was distilled by a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA).
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7

Synthesis of Transition Metal-Doped TiO2 Photocatalysts

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EDA, acetonitrile,
EG, concentrated ammonium hydroxide (NH3·H2O, 28 wt %), ethanol, hydrogen peroxide (H2O2, 30 wt %), manganese chloride tetrahydrate (MnCl2·4H2O), cobalt chloride hexahydrate (CoCl2·6H2O), nickel chloride hexahydrate (NiCl2·6H2O), copper chloride dehydrate (CuCl2·2H2O), and MB were all purchased from Sinopharm Chemical Reagent
Co., Ltd. (Shanghai, China). Titanium isopropoxide (98%) was obtained
from by J&K Scientific Ltd. (Beijing).
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8

Synthesis of Graphite Oxide-Based Nanocomposites

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Graphite oxide (GO) was supplied by Shenzhen Matterene Tech Co., Ltd (Shenzhen, China). Ferric chloride hexahydrate (FeCl3·6H2O), cobalt chloride hexahydrate (CoCl2·6H2O), urea, ethylene glycol, diglycol, polyvinylpyrrolidone (PVP, K30), ammonium hydroxide and ethanol were obtained from Sinopharm Chemical Reagent, China. And p-phenylenediamine was bought from Aladdin Bio-Chem Technology Co., Ltd, Shanghai, China. Deionized water was used throughout the experiments.
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9

Reagents and Materials for Bioactive Compound Analysis

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DL-malic acid and levofloxacin were purchased from Aladdin Biochemical Technology Co., Ltd., (Shanghai, China). All the inorganic reagents, including sodium chloride, potassium chloride, aluminum nitrate nonahydrate, manganese chloride tetrahydrate, ferric chloride, magnesium chloride hexahydrate, nickel chloride hexahydrate, cobalt chloride hexahydrate, calcium chloride, stannous chloride, lead (II) chloride, and barium chloride dehydrate, were of analytical grade and obtained from Sinopharm Chemical Reagent Co., Ltd., (Beijing, China). All the organic compounds, such as D-asparagine (D-Asn), L-glutamic acid (LGA), L-valine (L-Val), L-methionine (L-Met), L-asparagine (L-Asn), L-leucine (L-Leu), S-mandelic acid (S-MA), L-glutamine (L-Glu), and L-ascorbic acid (AA) were supplied by Aladdin Industrial Corporation. The ultrapure water used was directly obtained from the lab with a Milli-Q direct water purification system.
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10

Synthesis of Dopamine-Functionalized Metal Nanoparticles

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All of the
reagents were adopted without further purification as received. Tin
chloride pentahydrate (SnCl4·5H2O), cobalt
chloride hexahydrate (CoCl2·6H2O), trisodium
citrate dihydrate (C6H5Na3O7), sodium hydroxide (NaOH), potassium permanganate (KMnO4), and tris (hydroxymethyl) aminomethane (C4H11NO3) were obtained from Sinopharm Chemical Reagent Co.,
Ltd. (China). All of the dopamine hydrochloride (C8H11NO2·HCl) was purchased from Aladdin Industrial
Inc.
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