HL, HB, HQ and ZZ were purchased from Nanning Wanyaotang Pharmaceutical Co., Ltd. (Nanning, China) and authenticated by Professor Songji Wei (College of Pharmacy, Guangxi University of Chinese Medicine, China). The voucher specimens, deposited at the Guangxi University of Chinese Medicine, were HL-2017-0401, HQ-2017-0402, HB-2017-0403, and ZZ-2017-0104 for HL, HQ, HB, and ZZ, respectively.
Briefly, 300 g of HL, 200 g of HB, 200 g of HQ, and 300 g of ZZ were extracted twice, for 2 h each time, by refluxing in water according to the weight ratio of 15:1 of water to herbal. Then, the aqueous solution was combined, then filtered and concentrated in a rotary evaporator to a fluid extract with a relative density of about 1.05–1.20 (measured at 50–60 ℃), and then was stored in a refrigerator at 4 ℃. The content of berberine hydrochloride, a kind of active compound, in HJD extract was 20.39 mg/g, which was analyzed by high-performance liquid chromatography (Alliance 2695, Waters, USA) on a Inertsil ODS-2 C18 analytical column (4.6 mm × 250 mm, 5 μm) with elution by acetonitrile-0.05 moL/L phosphoric acid aqueous solution (50:50), a flow rate at 1.0 mL/min and the detection wavelength of 345 nm. The column temperature was 30 ℃, and the injection volume was 10 μL.
Briefly, 300 g of HL, 200 g of HB, 200 g of HQ, and 300 g of ZZ were extracted twice, for 2 h each time, by refluxing in water according to the weight ratio of 15:1 of water to herbal. Then, the aqueous solution was combined, then filtered and concentrated in a rotary evaporator to a fluid extract with a relative density of about 1.05–1.20 (measured at 50–60 ℃), and then was stored in a refrigerator at 4 ℃. The content of berberine hydrochloride, a kind of active compound, in HJD extract was 20.39 mg/g, which was analyzed by high-performance liquid chromatography (Alliance 2695, Waters, USA) on a Inertsil ODS-2 C18 analytical column (4.6 mm × 250 mm, 5 μm) with elution by acetonitrile-0.05 moL/L phosphoric acid aqueous solution (50:50), a flow rate at 1.0 mL/min and the detection wavelength of 345 nm. The column temperature was 30 ℃, and the injection volume was 10 μL.