Acquity uplc 1 class ftn system

Tryptophan, kynurenine, KYNA, 3-hydroxykynurenine (3-HK), QUIN, and picolinic acid were analyzed with a Xevo TQ-XS triple quadrupole mass spectrometer (Waters, Manchester, UK) equipped with a Z-spray electrospray interface and a Waters Acquity UPLC I-Class FTN system (Waters, Milford, MA) using a validated method previously described [51 (link)].

LC separation
was performed on an ACQUITY UPLC I-Class FTN system, with a Binary
Solvent Manager with column selection valves (Waters Corporation).
Ten microliters of the enriched sample was injected onto an ACQUITY
Premier Peptide BEH C18 column (2.1 mm × 30 (or 50) mm, 1.7 μm,
300 Å) column (Waters Corporation). Peptide separation was performed
using a gradient elution of mobile phase A containing LC–MS-grade
deionized water with 0.1% (v/v) formic acid and mobile phase B containing
LC–MS-grade acetonitrile with 0.1% (v/v) formic acid. A Xevo
TQ-XS tandem MS (Waters Corporation, Wilmslow, UK), operating in positive
electrospray ionization, was used for the detection and quantification
of the peptides. Details on the LC gradients and MS parameters can
be retrieved from Supplementary Methods and Table S1.
Skyline (version 21.1) was used to process the raw
LC–MS data using a template file containing the six target
peptides. Peak integration boundaries were automatically set on the
heavy standard and manually reviewed before exporting a report containing
the peptide-modified sequence, transition, area, and height among
others.
The mass spectrometry MRM and DIA-MS proteomics data
have been
deposited to the ProteomeXchange Consortium via the Panorama Public
partner repository with the dataset identifier PXD031401.^{21 (link),22 (link)}

LC–MS/MS analyses were performed on a Waters AcquityUPLC I-class FTN system coupled to a Xevo TQ-S Triple Quadrupole mass Spectrometer (Waters, Milford, MA, USA) operating in positive electrospray ionization mode with the electrospray-ionization mode(ESI) source. The analytical column was an Acquity UPLC^® HSS T3 column (100 mm × 2.1 mm I.D., 1.8 µm particle size). The column oven was set at 40 °C. The flow rate of the mobile phase was 0.4 mL/min and the injection volume was 10 µL. Eluent A was water and eluent B was methanol, both containing 0.5% acetic acid and 1mM ammonium acetate. For mycotoxin elution, the proportion of eluent B was kept constant at 2% for 2 min, then linearly increased to 50% in 4 min, then to increase to 80% over the next 2 min. Finally, it was raised to 98% and kept constant for 3 min. The column was re-equilibrated with 2% eluent B for 3.5 min. The parameters used for data acquisition in selected reaction monitoring (SRM) are reported in Table S1 (Supplementary Materials). Examples of selected ion chromatograms of spiked and naturally contaminated dust samples are shown in Figures S1 and S2 (Supplementary Materials). MasslynxTM version 4.1 and Quanlynx^® version 4.1 software (Waters, Milford, MA, USA) were used for data acquisition and processing.