The samples of Antarctic krill were provided by Dalian Marine Fisheries Group Cop.of Liaoning province (Dalian, Liaoning, China), which were transported to the laboratory at −20°C and stored at −50°C until use.
The standard mixtures of 37 fatty acid methyl esters (FAMEs), retinol and α‐, β‐, γ‐, and δ‐tocopherols (purity > 95%) were purchased from Shanghai ANPEL Science Instrument Co., Ltd. (Shanghai, China). Compound proteinase and acidic protease were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China). Alcalase and neutrase were purchased from JiangSu RuiYang BioTech (Jiangsu, China). Papain, pancreatin and anhydrous ethanol, petroleum ether (30~60°C) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
The standard mixtures of 37 fatty acid methyl esters (FAMEs), retinol and α‐, β‐, γ‐, and δ‐tocopherols (purity > 95%) were purchased from Shanghai ANPEL Science Instrument Co., Ltd. (Shanghai, China). Compound proteinase and acidic protease were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China). Alcalase and neutrase were purchased from JiangSu RuiYang BioTech (Jiangsu, China). Papain, pancreatin and anhydrous ethanol, petroleum ether (30~60°C) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).