Anandamide aea

The serine hydrolase activity-based protein profiling probe (ABPP probe), FP-TAMRA, was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Small interfering RNAs (FlexiTube SI02736706 for fatty acid amide hydrolase (FAAH) gene knockdown and Allstars Negative Control) were purchased from Qiagen (Tokyo, Japan), and transfection reagent Lipofectamine RNAiMAX was purchased from Thermo Fisher Scientific. All inhibitors, antagonists, and reagents, including PF3845, URB597, SR141716, SR144528, GW9662, GW6471, O1918, anandamide (AEA), and arachidonic acid (AA), were purchased from Cayman Chemical (Ann Arbor, MI, USA).
Radioactive anandamide [ethanolamide-1,2-¹⁴C] was purchased from American Radiolabeled Chemicals, Inc (Saint Louis, MO, USA). Other chemicals including lipopolysaccharide were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Anandamide (AEA) (>98.0% purity) was purchased from Cayman Chemical (Ann Arbor, MI, USA) and dissolved in absolute ethanol at a concentration of 10 mg/mL. Respective dilutions of ethanol (0.0156–0.5%) as well as untreated bacteria were used as controls in this study.

All chemicals and reagents were purchased as follows: N-arachidonoyl ethanolamine or Anandamide (AEA) from Cayman Chemicals (Ann Arbor, Michigan, United States); Staphylococcus Enterotoxin B (SEB): from Toxin Technologies (Sarasota, FL); RPMI 1640 supported with L-glutamine: from Corning (New York, NY, United States); Fetal Bovine Serum, Penicillin and Streptomycin: from Invitrogen Life Technologies (CA, United States). True Nuclear Transcription Factor Buffer Set was purchased from Biolegend (San Diego, CA) while Fc Block reagent was purchased from BD Pharmingen (San Diego, CA). EasySep mouse MDSCs (CD11bGr1) selection kit was purchased from STEMCELL technologies (Seattle, WA) for purification of MDSCs. miScript SYBR green PCR kit, miScript primer assays kit, RNA easy, and miRNA easy kit were purchased from Qiagen (Valencia, CA). Taq DNA polymerase kit was purchased from Invitrogen Life Technologies (Carlsbad, CA).