Axioskope 2 microscope

Manufactured by Zeiss

Sourced in Germany

The Axioskope 2 is a light microscope designed for routine observation and analysis. It features a high-quality optical system that provides clear, high-resolution images. The microscope is equipped with a range of objectives and eyepieces to accommodate various magnification requirements. The Axioskope 2 is a versatile instrument suitable for a variety of applications in research, education, and industrial settings.

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Lab products found in correlation

Image pro plus software 7, by Media Cybernetics (2 mentions) Zen 2009 light edition software, by Zeiss (2 mentions) Lsm700, by Nikon (2 mentions) Nis element 5, by Nikon (2 mentions) Axiocammr3 camera, by Zeiss (2 mentions) A1 confocal microscope, by Nikon (2 mentions) Array scan xti platform, by Thermo Fisher Scientific (1 mentions) Nestin, by Proteintech (1 mentions) Stro 1, by R&D Systems (1 mentions) Collagen 1 coated glass slides, by BD (1 mentions) Cd146, by Merck Group (1 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Triton x 100, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

6 protocols using axioskope 2 microscope

Fluorescence Imaging for Single-Cell Analyses

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A video imaging setup consisting of an Axioskope 2 microscope (Zeiss, Oberkochen, Germany) and a Polychrome IV (Till Photonics, GmbH, Martinsried, Germany) light source was used for single-cell experiments. Fluorescence images were collected by a cooled CCD videocamera (PCO Computer Optics GmbH, Kelheim, Germany). The “Vision” software (Till Photonics) was used to control the acquisition protocol and to perform data analysis (Vangelista et al., 2005 (link); Codazzi et al., 2006 (link)). This instrument was used for both fura-2-based calcium analyses and mBCl-based GSH measurements.
The automated ArrayScan XTI platform (Thermo Fisher Scientific) was used for fluo-8-based calcium measurements, reactive oxygen species (ROS), and TMRM-mitochondrial membrane potential analyses.

Rosato C., Bettegazzi B., Intagliata P., Balbontin Arenas M., Zacchetti D., Lanati A., Zerbini G., Bandello F., Grohovaz F, & Codazzi F. (2022). Redox and Calcium Alterations of a Müller Cell Line Exposed to Diabetic Retinopathy-Like Environment. Frontiers in Cellular Neuroscience, 16, 862325.

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Multiparametric Imaging of Atherosclerotic Lesions

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Movat, IL1R1, MMP3, Ter119 and Sudan IV staining of brachiocephalic arteries were imaged using a Zeiss Axioskope2 microscope equipped with an AxioCamMR3 camera. Image acquisition was performed with AxioVision40 version 4.6.3.0 software (Carl Zeiss Imaging Solution). Digitized images were analyzed with Image Pro Plus Software 7.0 (Media Cybernetics). Immunofluorescent staining was imaged using either a Zeiss LSM700 or a Nikon A1 confocal microscope to acquire a series of eight z-stack images at 1-μm intervals. Zen 2009 Light Edition Software (Zeiss) or NIS-Element 5.02 Software (Nikon) were used for analysis of each z-stack image and single-cell counting was performed for phenotyping and quantifying the cell population comprised within the 30μm thick layer proximal to the lumen (i.e., fibrous cap area). Assessment of YFP⁺, ACTA2⁺, and LGALS3⁺ areas (normalized to lesion or fibrous cap area) was performed using maximal intensity projection images and the analysis was done using Image Pro Plus Software 7.0 (Media Cybernetics). Maximal intensity projection of representative images were used to generate the representative images included in the figures and Adobe Photoshop was used to process and format images.

Gomez D., Baylis R.A., Durgin B.G., Newman A.A., Alencar G.F., Mahan S., St. Hilaire C., Muller W., Waisman A., Francis S.E., Pinteaux E., Randolph G.J., Gram H, & Owens G.K. (2018). Interleukin-1β promotes atheroprotective changes of advanced atherosclerotic lesions in mice. Nature medicine, 24(9), 1418-1429.

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Quantifying Vacuolization in Fly Brains

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Flies were obtained and aged as described for the phototaxis experiments. Paraffin sections for light microscopy were prepared and analyzed for vacuole formation as described in Botella et al. (2003) (link) and Sunderhaus and Kretzschmar (2016) (link). Briefly, whole flies were fixed in Carnoy’s solution and dehydrated in an ethanol series followed by incubation in methyl benzoate before embedding in paraffin. Sections were cut at 7 μm and analyzed with a Zeiss Axioskope 2 microscope using the auto-fluorescence caused by the dispersed eye pigment. To quantify the vacuolization, we photographed sections at the level of the great commissure and numbered the pictures for a double-blind analysis. The area of the vacuoles in the deutocerebral neuropil was then calculated in ImageJ as total pixel number, converted into μm², and the genotype determined. Statistical analysis was done using GraphPad Prism and one-way ANOVA with Dunnett’s post hoc tests.

Sunderhaus E.R., Law A.D, & Kretzschmar D. (2019). Disease-Associated PNPLA6 Mutations Maintain Partial Functions When Analyzed in Drosophila. Frontiers in Neuroscience, 13, 1207.

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Multiparametric Imaging of Atherosclerotic Lesions

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Characterization of Stem Cell Markers

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TSPC at passage 6 were plated and cultured on 20 μg/ml collagen 1-coated glass slides (BD Bioscience, USA) for 48 h. Then, the cells were fixed with 4% paraformaldehyde (Merck, Germany), permeabilized with Triton X100 (Sigma-Aldrich) and blocked with 3% BSA (PAA, USA). Primary antibodies against CD146 (Millipore, USA), Nestin (Proteintech, USA) and STRO-1 (R&D Systems, USA) were applied overnight at 4°C. Next, secondary Alexa Flour 488-conjugated antibodies and DAPI were used (all Life technologies, USA). As negative control were used cell-seeded slides which were incubated only with secondary Alexa Flour 488-conjugated antibodies and DAPI. Photomicrographs were taken with Axiocam MRm camera on Axioskope 2 microscope (Carl Zeiss, Germany). Staining procedures were reproduced at least twice.

Popov C., Burggraf M., Kreja L., Ignatius A., Schieker M, & Docheva D. (2015). Mechanical stimulation of human tendon stem/progenitor cells results in upregulation of matrix proteins, integrins and MMPs, and activation of p38 and ERK1/2 kinases. BMC Molecular Biology, 16, 6.

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Quantifying Collagen Birefringence in Tendons

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Organized collagen type I fibers have strong birefringence property when exposed to polarized light; hence, they can be visualized in orange to yellow colors using microscopy. All 10 animals per group were prescreened and four representative animals/group were selected for the best quality of the H&E slides allowing for optimal and identical setting of the polarized microscopy imaging. Each animal was represented with one slide consisting of three sections. Microscopy was performed with 10× objective supplemented with a polarized filer mounted on Axioskope 2 microscope (Carl Zeiss). For optimal imaging the transmission axis of the analyzer was with an angle of 54° to the axis of the polarizer in the analyzes of all samples. In order to cover the entire tendon territory approximately 50 consecutive images/section were taken manually in a mosaic manner and digitally stitched with Adobe Photoshop CS5 software (Adobe System, CA, USA). Next, total Achilles tendon area (denominator) was measured in pixel with 'magic wand' tool and by using 'color selecting' tool the yellow-to-orange-positive pixels (numerator), corresponding to highly organized collagen areas, were automatically quantified. Final data were expressed as a percentage of collagen birefringence and for each study group mean values and standard deviations were calculated.

Hsieh C.F., Alberton P., Loffredo-Verde E., Volkmer E., Pietschmann M., Müller P., Schieker M, & Docheva D. (2016). Scaffold-free Scleraxis-programmed tendon progenitors aid in significantly enhanced repair of full-size Achilles tendon rupture. Nanomedicine (London, England), 11(9).

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