Ab137638

Manufactured by Abcam

Sourced in United Kingdom

Ab137638 is a laboratory product available from Abcam. It is a laboratory instrument or equipment, but further details about its core function are not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

Ab64256, by Abcam (2 mentions) Ab16502, by Abcam (2 mentions)

2 protocols using ab137638

TRIM27 and NF-kB Expression Analysis

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Tissues were embedded in paraffin. Slices (5 μm) were treated in H₂O₂ (3%) dissolved in methanol and 5% normal horse serum before staining. Sections were incubated with the following primary antibodies overnight at 4 °C: anti-TRIM27 antibody (ab137638; Abcam, UK) and anti-NF-KB antibody (ab16502; Abcam, UK). The sections were then incubated with secondary goat anti-rabbit IgG (1:100; ab64256; Abcam) for 30 min at room temperature. The sections were visualized with diaminobenzidine and hematoxylin. Cells with > 25% positive staining were defined as having “high expression.”

Xiao C., Zhang W., Hua M., Chen H., Yang B., Wang Y, & Yang Q. (2021). TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway. BMC Cancer, 21, 841.

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Immunohistochemical Analysis of TRIM27 and NF-κB

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Tissues were embedded in para n. Slices (5 μm) were treated in H 2 O 2 (3%) dissolved in methanol and 5% normal horse serum before staining. Sections were incubated with the following primary antibodies overnight at 4°C: anti-TRIM27 antibody (ab137638; Abcam, UK) and anti-NF-KB antibody (ab16502; Abcam, UK). The sections were then incubated with secondary goat anti-rabbit IgG (1:100; ab64256; Abcam) for 30 min at room temperature. The sections were visualized with diaminobenzidine and hematoxylin. Cells with >25% positive staining were de ned as having "high expression."

Xiao C., Zhang W., Hua M., Chen H., Yang B., Wang S., & Yang Q. (2021). TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway.

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