Flies were lysed in radioimmunoprecipitation assay buffer (Thermo Fisher Scientific) and proteins were then separated by SDS‐PAGE using standard procedures (Wang et al., 2005 (link)). The antibodies used were mouse anti‐AMPK (Abcam), rabbit anti‐phospho‐AMPK (Cell Signaling), rabbit anti‐phospho‐Drosophila p70 S6 Kinase (Cell Signaling), and mouse anti‐α Tubulin (GeneTex). Protein signals were detected with horseradish peroxidase‐conjugated secondary antibodies and ECL reagent (Thermo Fisher Scientific). Immunoblots were quantified using Image J software.
Mouse anti ampk
Manufactured by Abcam
Sourced in Japan
Mouse anti‐AMPK is a primary antibody that recognizes the AMP-activated protein kinase (AMPK) in mouse samples. AMPK is a cellular energy sensor that plays a crucial role in the regulation of metabolism. This antibody can be used to detect and study AMPK expression and activation in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti phospho ampk, by Cell Signaling Technology (1 mentions)
Ecl reagent, by Thermo Fisher Scientific (1 mentions)
Radioimmunoprecipitation assay buffer, by Thermo Fisher Scientific (1 mentions)
Mouse anti α tubulin, by GeneTex (1 mentions)
Rabbit anti ulk1, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho ampkα t172, by Cell Signaling Technology (1 mentions)
Mouse anti mfn1, by Abcam (1 mentions)
Mouse anti tim23, by BD (1 mentions)
Mouse anti vdac1, by Abcam (1 mentions)
Rabbit anti tom20, by Cell Signaling Technology (1 mentions)
Rabbit anti cox 4, by Cell Signaling Technology (1 mentions)
Peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Rabbit anti ha, by Cell Signaling Technology (1 mentions)
Mouse anti ndufs3, by Abcam (1 mentions)
Rabbit anti flag, by Cell Signaling Technology (1 mentions)
Mouse anti tubulin, by Developmental Studies Hybridoma Bank (1 mentions)
2 protocols using mouse anti ampk
1
AMPK and S6 Kinase Immunoblotting
2
Comprehensive Immunoblot Analysis of Mitochondrial Proteins
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For immunoblot analysis, the following antibodies were used: mouse anti-MFN1 (Abcam), rabbit anti-TOM20 (Cell Signaling Technology), mouse anti-TIM23 (BD Biosciences), rabbit anti-COXIV (Cell Signaling Technology), rabbit anti-UCHL1 (Cell Signaling Technology), mouse anti-tubulin (Developmental Studies Hybridoma Bank), rabbit anti–phospho-AMPKα (T172, Cell Signaling Technology), rabbit anti–phoshpo-ULK1 (S555, Cell Signaling Technology), mouse anti-AMPK (Abcam), rabbit anti-ULK1 (Cell Signaling Technology), rabbit anti-FUNDC1 (Novus Biologicals), mouse anti-VDAC1 (Abcam), mouse anti-NDUFS3 (Abcam), rabbit anti-Flag (Cell Signaling Technology), rabbit anti-HA (Cell Signaling Technology), mouse anti-Myc (MBL, Japan), mouse anti-TRIM63 (Santa Cruz Biotechnology), and rabbit anti-PKM antibody (Cell Signaling Technology). Peroxidase-conjugated secondary antibodies were purchased from the Jackson laboratory.
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